Protective effect of diosmetin on in vitro cell membrane damage and oxidative stress in cultured rat hepatocytes

Villa, Pia; Cova, D.; Francesco, Laura De; Guaitani, A.; Palladini, G.; Perego, Rita

doi:10.1016/0300-483x(92)90101-j

Cited by 37 publications

(18 citation statements)

References 24 publications

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“…Similar results were obtained for diosmentin, the main metabolite of the flavonoid diosmin (Villa et al 1992). Diosmentin inhibited the release of the enzymes lactate dehydrogenase and aspartate-aminotransferase from primary hepatocyte cell cultures induced by non-oxidant (erythromycin estolate) and pro-oxidant (tert-butylhydroperoxide) aggressors (Villa et al 1992). A direct protective action of flavonoids in vivo is supported by the observation that erythrocytes obtained from rats given a flavanoid-rich meal (cocoa extract) showed a reduced susceptibility to free radical-mediated hemolysis (Zhu et al 2002).…”

Section: Polyphenols Can Interact and Reduce The Access Of Oxidants Asupporting

confidence: 73%

“…In agreement with results shown in Table I, while kaempferol and morin were strong inhibitors, naringenin and taxifolin had minor effects against osmotic stress. Similar results were obtained for diosmentin, the main metabolite of the flavonoid diosmin (Villa et al 1992). Diosmentin inhibited the release of the enzymes lactate dehydrogenase and aspartate-aminotransferase from primary hepatocyte cell cultures induced by non-oxidant (erythromycin estolate) and pro-oxidant (tert-butylhydroperoxide) aggressors (Villa et al 1992).…”

Section: Polyphenols Can Interact and Reduce The Access Of Oxidants Asupporting

confidence: 70%

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Flavonoid‐membrane Interactions: A Protective Role of Flavonoids at the Membrane Surface?

Oteiza

Erlejman

Verstraeten

et al. 2005

Journal of Immunology Research

328

186

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Flavonoids can exert beneficial health effects through multiple mechanisms. In this paper, we address the important, although not fully understood, capacity of flavonoids to interact with cell membranes. The interactions of polyphenols with bilayers include: (a) the partition of the more non-polar compounds in the hydrophobic interior of the membrane, and (b) the formation of hydrogen bonds between the polar head groups of lipids and the more hydrophilic flavonoids at the membrane interface. The consequences of these interactions are discussed. The induction of changes in membrane physical properties can affect the rates of membrane lipid and protein oxidation. The partition of certain flavonoids in the hydrophobic core can result in a chain breaking antioxidant activity. We suggest that interactions of polyphenols at the surface of bilayers through hydrogen bonding, can act to reduce the access of deleterious molecules (i.e. oxidants), thus protecting the structure and function of membranes.

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Section: Polyphenols Can Interact and Reduce The Access Of Oxidants Asupporting

confidence: 73%

Section: Polyphenols Can Interact and Reduce The Access Of Oxidants Asupporting

confidence: 70%

Flavonoid‐membrane Interactions: A Protective Role of Flavonoids at the Membrane Surface?

Oteiza

Erlejman

Verstraeten

et al. 2005

Journal of Immunology Research

328

186

View full text Add to dashboard Cite

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“…In addition, DIOS caused significant but not dramatic decrease of ROS/protein ratios in co-treated (both OTA and DIOS) cells compared to the simply OTA-exposed cells. In vitro antioxidant effect of DIOS was reported in previous studies [47,48]. On the other hand, DIOS has much lower radical scavenging activity in cell cultures compared to other flavonoids (e.g.…”

Section: Diosmetin Versus Atp Depleting Effect Of Ochratoxin Amentioning

confidence: 57%

Flavonoid diosmetin increases ATP levels in kidney cells and relieves ATP depleting effect of ochratoxin A

Poór

Veres

Jakus

et al. 2014

Journal of Photochemistry and Photobiology B: Biology

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a b s t r a c tDiosmetin (DIOS) is a flavone aglycone commonly occurring in citrus species and olive leaves, in addition it is one of the active ingredients of some medications. Based on both in vitro and in vivo studies several beneficial effects are attributed to DIOS but the biochemical background of its action seems to be complex and it has not been completely explored yet. Previous investigations suggest that most of the flavonoid aglycones have negative effect on ATP synthesis in a dose dependent manner. In our study 17 flavonoids were tested and interestingly DIOS caused a significant elevation of intracellular ATP levels after 6-and 12-h incubation in MDCK kidney cells. In order to understand the mechanism of action, intracellular ATP and protein levels, ATP/ADP ratio, cell viability and ROS levels were determined after DIOS treatment. In addition, impacts of different enzyme inhibitors and effect of DIOS on isolated rat liver mitochondria were also tested. Finally, the influence of DIOS on the ATP depleting effect of the mycotoxin, ochratoxin A was also investigated. Our major conclusions are the followings: DIOS increases intracellular ATP levels both in kidney and in liver cells. Inhibition of glycolysis or citric acid cycle does not decrease the observed effect. DIOS-induced elevation of ATP levels is completely abolished by the inhibition of ATP synthase. DIOS is able to completely reverse the ATP-depleting effect of the mycotoxin, ochratoxin A. Most probably the DIOS-induced impact on ATP system does not originate from the antioxidant property of DIOS. Based on our findings DIOS may be promising agent to positively influence ATP depletion caused by some metabolic poisons.

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“…It might be a non -specific effect, since BPE also protect the hepatocyte in CCl 4 liver damage 2 , but it might also have a specific effect , protecting against the detergent action of retained bile salts and /or againt the cholestasis induced by mono hydroxylated bile acids. From the many known components of B. pilosa the flavonoids could be the active principle, since some of them as silymarin 42 and diosmin protect against the membrane damage induced by the cholestatic agent, erythromycin estolate 43 . Further research is needed to identify the active substance and its mode of action.…”

Section: Also Called C Forskohlii)mentioning

confidence: 99%

An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

et al. 2009

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Purpose:To test the hepatoprotective effect of water extract from Bidens Pilosa L. (BPE) in cholestatic liver disease induced by ligature and resection of the common bile ducts (LRBD) in young rats. Methods: We studied four groups of ten 21 days old (P21) Wistar rats, Group SW: sham operation and water; Group SD: sham operation and BPE (160 mg of fresh leaves/100 g of body weight/day); Group LW: LRBD and water and Group LD: LRBD and BPE daily. Pentobarbital sleeping time (PST) and serum activities of aspartate aminotransferase (AST) and of alanine aminotransferase (ALT) were determined after the sacrifice (P70). A Ruwart's score for hepatic fibrosis (RS) was given to each animal. Were employed two way ANOVA and the test of Tukey or a non-parametric test for multiple comparisons. Results: There were statistically significant differences between LW and LD in the measurements of the PST ((means LW=390; LD=173), AST (means LW=8, LD=5), ALT (medians LW=2; LD=1) e RS (medians LW=2; LD=1). Conclusion: BPE could be used in the phytotherapy of the hepatic damage induced by chronic obstructive cholestasis, because protects liver function, decreases the rate of necrosis and liver fibrosis in cholestatic liver disease. Key words: Bidens. Phytotherapy. Cholestasis, Extrahepatic. Liver Cirrhosis, Biliary. Rats. RESUMO Objetivo:Testar o efeito hepatoprotetor do extrato aquoso de Bidens pilosa L. (EBP) na doença hepática induzida pela ligadura e ressecção do ducto biliar comum (LRDBC) em ratos jovens. Métodos: Estudamos ratos Wistar com 21º. dia de vida (P21) divididos em quatro grupos de 10 animais, Grupo SA: operação simulada e água; Grupo SD: operação simulada e EBP (160mg de folhas frescas/100g de peso corporal/dia); Grupo LA: LRDBC e água e Grupo LD: LRDBC e EBP diariamente. O tempo de sono por pentobarbital (TSP), aspartato (AST) e alanina (ALT) aminotransferase foram determinadas após o sacrifício (P70). O Score de Ruwart (SR) para fibrose hepática foi atribuído para cada animal. Foi realizada análise de variância com dois fatores e pelo teste de Tukey para comparações múltiplas ou por teste não paramétrico. Resultados: Houve diferença estatisticamente significativa entre LA e LD nas variáveis: TSP (médias LA=390; LD=173), AST (médias LA=8, LD=5), ALT (medianas LA=2; LD=1) e SR (medianas LA=2; LD=1). Conclusão: EBP poderá ser empregado na fitoterapia da doença hepática induzida pela colestase obstrutiva crônica, pois protege a função hepática, diminui a velocidade de necrose e a intensidade da fibrose hepática na obstrução biliar extra-hepática.

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Protective effect of diosmetin on in vitro cell membrane damage and oxidative stress in cultured rat hepatocytes

Cited by 37 publications

References 24 publications

Flavonoid‐membrane Interactions: A Protective Role of Flavonoids at the Membrane Surface?

Flavonoid‐membrane Interactions: A Protective Role of Flavonoids at the Membrane Surface?

Flavonoid diosmetin increases ATP levels in kidney cells and relieves ATP depleting effect of ochratoxin A

An acqueous extract of Bidens pilosa L. protects liver from cholestatic disease: experimental study in young rats

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