Protective effect of dipeptidyl peptidase-4 inhibitors in testicular torsion/detorsion in rats: a possible role of HIF-1α and nitric oxide

Abdelzaher, Walaa Yehia; Rofaeil, Remon Roshdy; Ali, Doaa M.; Attya, Mina Ezzat

doi:10.1007/s00210-019-01765-5

Cited by 30 publications

(32 citation statements)

References 61 publications

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“…Serum testosterone levels in the torsion/detorsion groups were found to be significantly decreased when compared to the control group. The decrease in testosterone has been similarly reported in previous testicular ischemia studies (Abdelzaher et al, 2020;Shakoohi et al, 2018;Kheirollahi et al, 2018). The decrease in testosterone may be associated with damage to the Leydig cells (Benny et al, 2019).…”

Section: Discussionsupporting

confidence: 71%

“…However, excessive free oxygen radicals can impair sperm function (Saalu, 2010). In the literature, it has been reported that testicular ischemia/reperfusion events cause a decrease in SOD value (Abdelzaher et al, 2020;Dejban et al, 2019;Ganjani et al, 2020;Shakoohi et al, 2018;Un et al, 2015). In our study, the SOD value decreased significantly in the torsion/detorsion groups compared to the control group.…”

Section: Discussionsupporting

confidence: 51%

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Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

Belhan¹,

Yıldırım²,

Kayikci³

et al. 2021

Turk J Zool

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The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats (10-12 weeks of age weighing 280-300 grams) were randomly divided into five groups.Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin (250 mg/kg) group; and torsion 3 h / detorsion 24 h + silymarin (250 mg/kg) group. In the study, 720 ° torsion was applied to the left testicle. At the end of the study, blood was collected from the rats and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha (Tnf-α) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities decreased, while malondialdehyde (MDA) levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 51%

Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

Belhan¹,

Yıldırım²,

Kayikci³

et al. 2021

Turk J Zool

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“…Endothelial cell migration and tube formation are also impaired, an effect that is accompanied with a decrease in the expression of VEGF, VE-cadherin, and MMP2 [98,99], suggesting that oxidative stress affects the angiogenesis and regenerative capacity of micro-vessels in reperfused myocardium. Mechanistically, mtROS overproduction seems to be associated with a decrease in the levels of total antioxidant capacity and SOD [100]. Of note, the increase in mtROS in endothelial cells under I/R injury may also result from excessive inflammation response because myeloperoxidase (MPO) expression, a neutrophil infiltration marker, is upregulated in reperfused heart [101,102].…”

Section: Mitochondrial Oxidative Stressmentioning

confidence: 99%

Pathological Roles of Mitochondrial Oxidative Stress and Mitochondrial Dynamics in Cardiac Microvascular Ischemia/Reperfusion Injury

Zhou

Toan

2020

Biomolecules

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Mitochondria are key regulators of cell fate through controlling ATP generation and releasing pro-apoptotic factors. Cardiac ischemia/reperfusion (I/R) injury to the coronary microcirculation has manifestations ranging in severity from reversible edema to interstitial hemorrhage. A number of mechanisms have been proposed to explain the cardiac microvascular I/R injury including edema, impaired vasomotion, coronary microembolization, and capillary destruction. In contrast to their role in cell types with higher energy demands, mitochondria in endothelial cells primarily function in signaling cellular responses to environmental cues. It is clear that abnormal mitochondrial signatures, including mitochondrial oxidative stress, mitochondrial fission, mitochondrial fusion, and mitophagy, play a substantial role in endothelial cell function. While the pathogenic role of each of these mitochondrial alterations in the endothelial cells I/R injury remains complex, profiling of mitochondrial oxidative stress and mitochondrial dynamics in endothelial cell dysfunction may offer promising potential targets in the search for novel diagnostics and therapeutics in cardiac microvascular I/R injury. The objective of this review is to discuss the role of mitochondrial oxidative stress on cardiac microvascular endothelial cells dysfunction. Mitochondrial dynamics, including mitochondrial fission and fusion, are critically discussed to understand their roles in endothelial cell survival. Finally, mitophagy, as a degradative mechanism for damaged mitochondria, is summarized to figure out its contribution to the progression of microvascular I/R injury.

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“…The incidence of testicular torsion is up to 158, which mostly occurs in teenagers under 24 years old [2][3][4][5][6]. Clinically, because it is di cult to distinguish the disease from scrotal in ammation in the early stage [1,2,7,8], domestic data show that the misdiagnosis rate of testicular torsion can be as high as 67.6%. TT is very easy to cause misdiagnosis and torsion testicular ischemia time is too long, tissue necrosis [9], and nally can only carry out surgery to remove necrotic testicles, resulting in testicular loss in patients; this disease is also one of the most common causes of testicular loss in adults, so it should be diagnosed and treated as soon as possible.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

Tian

Zhang

Yang

et al. 2021

Preprint

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Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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Protective effect of dipeptidyl peptidase-4 inhibitors in testicular torsion/detorsion in rats: a possible role of HIF-1α and nitric oxide

Cited by 30 publications

References 61 publications

Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

Investigation of the effect of silymarin on oxidative DNA damage and inflammatory markers in ischemia/reperfusion injury following experimental testicular torsion/detorsion in rats

Pathological Roles of Mitochondrial Oxidative Stress and Mitochondrial Dynamics in Cardiac Microvascular Ischemia/Reperfusion Injury

Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

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