Protective Effect of Encapsulated Nanocurcumin- PEGOA against Oxidative Damage on Human Mesenchymal Stem Cells Exposed to Hydroquinone as a Risk Factor for Leukemia

Abstract

Introduction: Benzene a well-known environmental pollutant is a human carcinogen which is involved in the manifestation of a number of malignancies. Activation of benzene and its reactive metabolites such as hydroquinone (HQ) leads to continuous production of reactive oxygen species (ROS), causing oxidative Stress. Curcumin, the yellow pigment of curcuma longa, has been shown to possess antioxidant activity in vitro and in vivo. However, poor bioavailability is the major drawback about of this drug. By using dendrosome, a nontoxic nanoparticle, we tried to deal with this problem. In the present study, we have investigated the protective effects of encapsulated nanocurcumin-PEGOA (ENC) against the HQ-induced oxidative damage in human mesenchymal bone marrow stem cells (hMSCs). Methods: hMSCs were pre-treated with ENC and then exposed to HQ. Cell viability, intracellular ROS and lipid peroxidation extent were assessed by MTT assay, DCFH-DA fluorescent dye and thiobarbituric acid reactive substances (TBARS), respectively. Moreover, mRNA levels of antioxidant enzymes, catalase and hemeoxygenase-1 were evaluated by qRT-PCR. Results: The results showed that treatment of MSCs with ENC (10 µM) for 12 hours prior to HQ exposure, significantly attenuated the cell viability loss, suppressed the elevation of ROS and reduced the extent of lipid peroxidation caused by HQ compared with the control. Moreover, a significant increase in mRNA expression of antioxidant genes of catalase and hemeoxygenase-1 was observed after treatment with ENC. Conclusions: These results suggest that 10 µM ENC could protect hMSCs against toxic and oxidative effects of HQ. Therefore, ENC may have cancer protective effect.

Cytoprotective effects of antioxidant supplementation on mesenchymal stem cell therapy

“…Nazem et al, who examined the effect of hMSCs pretreatment with the polyethylene glycol octaacrylate encapsulated nanocurcumin, before the introduction of the oxidative stress situation. They well described that this pretreatment was accompanied by a reduced cell loss, reduced lipid peroxidation, and an elevated expression level for the antioxidant enzymes (mainly CAT and heme oxygenase-1;Nazem, Masoumi, Amirizadeh, Zolghadr, & Sadeghizadeh, 2017). In another study, Yousefi et al described the antioxidant potency of the nanocurcuminoids (a curcumin-based dietary complex composed of curcumin, desmethoxycurcumin, and methoxycurcumin) on the AD-MSCs which lead to the AD-MSCs' increased proliferation rate and decreased apoptosis.…”

Influence of Curcumin Nanocrystals on the Early Osteogenic Differentiation and Proliferation of Dental Pulp Stem Cells

“…The results exhibited that hMSC treatment with PEGOA nanocurcumin (10 μM) for 12 hours significantly reduced lipid peroxidation and increased the expression of antioxidant genes involving catalase and homoxygenase-1. These results indicate that PEGOA nanocurcumin can protect hMSC [41]. These results are intended for our study as a nontoxic effect of nanocurcumin and to protect and induce the growth of DPSCs.…”

Pomegranate seed extract enhances the inhibitory effect of adipose-derived mesenchymal stem cells on breast cancer cell line in co-culture conditions

“…Vitamin E, for example, greatly increases viability, inhibits inflammation, and reduces apoptosis in stem cells ( 22 ). Nanocurcumin increases the expression level of antioxidant genes and superoxide dismutase activity in bone marrow stem cells ( 23 24 ). Consequently, antioxidants appear to be able to improve stem cell efficacy.…”