2008
DOI: 10.1111/j.1745-7254.2008.00878.x
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Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species

Abstract: Aim: To investigate the anti-oxidant properties of esculetin (6,7-dihydroxycoumarin) against H 2 O 2 -induced Chinese hamster lung fibroblast (V79-4) damage. Methods: The radical scavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a p… Show more

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Cited by 103 publications
(62 citation statements)
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“…Esculetin is recognized as an inhibitor not only of the lipoxygenase and cyclooxygenase enzymatic systems but also for the neutrophil-dependent superoxide anion generation system (Fylaktakidou et al 2004). It has been shown to exhibit 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities (Kim et al 2008). The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H 2 O 2 .…”
Section: Resultsmentioning
confidence: 99%
“…Esculetin is recognized as an inhibitor not only of the lipoxygenase and cyclooxygenase enzymatic systems but also for the neutrophil-dependent superoxide anion generation system (Fylaktakidou et al 2004). It has been shown to exhibit 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities (Kim et al 2008). The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H 2 O 2 .…”
Section: Resultsmentioning
confidence: 99%
“…Lipid peroxidation was assayed by the measurement of related substances that react with thiobarbituric acid (TBARS) [43]. The V79-4 cells were seeded in a culture dish at a concentration of 1 × 105 cells/mL and treated with esculetin at 10 g/mL after 16 h plating and then hydrogen peroxide (H 2 O 2 ) 1 mmol/L was added to the plate after 1 h, which was incubated for a further 24 h. The cells were then washed with cold PBS, scraped, and homogenized in ice-cold 1.15% potassium chloride (KCl).…”
Section: Lipid Peroxidation Detectionmentioning
confidence: 99%
“…Assessment of intracellular ROS production ROS production was quantified by the DCFH-DA method [20] , based on the ROS-dependent oxidation of DCFH-DA to DCF. VSMCs grown in 96-well plates were treated with different concentrations of LA for 2 h prior to LPS (100 ng/mL) or FBS (5%) stimulation for 30 min at 37 °C.…”
Section: Introductionmentioning
confidence: 99%