Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model

Kim, Eun-A; Lee, Seung‐Hong; Ko, Chang-Ik; Cha, Seon-Heui; Kang, Min‐Cheol; Kang, Sung-Myung; Ko, Seok‐Chun; Lee, Wonwoo; Ko, Ju-Young; Lee, Ji-Hyeok; Kang, Ning; Oh, Jae-Young; Ahn, Ginnae; Jee, Young Heun; Jeon, You‐Jin

doi:10.1016/j.carbpol.2013.11.022

Cited by 101 publications

(67 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, the antioxidant effects of HPH‐III upon AAPH‐induced oxidative stress in the zebrafish embryo model, using DCF‐DA and acridine orange fluorescence dyes, were investigated (Figure ). 2′,7′‐dichlorofluorescein (DCF) is associated with ROS production (Kim et al, ). HPH‐III significantly reduced the total ROS level whose decrease was similar to the control by 42% decrease in the fluorescence intensity at 400 μg/ml (Figure a).…”

Section: Resultsmentioning

confidence: 99%

“…HPH‐III significantly reduced the total ROS level whose decrease was similar to the control by 42% decrease in the fluorescence intensity at 400 μg/ml (Figure a). Acridine orange is a fluorescent cationic dye useful for detecting apoptotic cells and especially stains necrotic and late apoptotic cells (Kim et al, ). A significant dose‐dependent cell death was also observed in groups treated with HPH‐III (Figure b).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Free radical scavenging activity of the peptide from the Alcalase hydrolysate of the edible aquacultural seahorse ( Hippocampus abdominalis )

Kim

Fernando

et al. 2019

J Food Biochem

View full text Add to dashboard Cite

Seahorses, Hippocampus abdominalis, have a long history in traditional Chinese medicine as an important healthy ingredient in foods. This study evaluated the antioxidant activity of an enzymatic hydrolysate prepared from a seahorse bred in Jeju, South Korea. Experiments were performed in vitro using electron spin resonance spectrometry (ESR) to determine the free radical scavenging activity and in vivo using a zebrafish model to determine the protective effects against 2,2‐azobis hydrochloride (AAPH)‐induced oxidative damage. H. abdominalis protein hydrolysate (HPH) exhibited peroxyl radical scavenging activity (IC50 = 0.58 mg/ml) generated by the water‐soluble AAPH (azo initiator of peroxyl radicals). HPH reduced dose‐dependently both intracellular reactive oxygen species (ROS) levels in AAPH‐induced cells and cell death in AAPH‐induced zebrafish embryos. The antioxidant peptide purified from HPH was identified as a tripeptide (alanine–glycine–aspartic acid) using Q‐TOF ESI mass spectroscopy. Thus, this study demonstrated that HPH contains antioxidant peptides that exhibit a strong antioxidant activity. Practical applications Hippocampus abdominalis is one of the largest seahorse species and cultivated in many countries. Because of its large body size compared to other seahorse species, H. abdominalis has acquired considerable consumer attraction in the global market. Owing to its biologically useful properties, it recently gained attention as the natural products obtained from H. abdominalis have varied applications in the field of medicine, health care products, and functional foods. Thus, commercial products of this particular seahorse species are popular among customers, especially in China. The purpose of this study was to evaluate the antioxidant property of H. abdominalism, cultured in a commercial seahorse farm in Jeju Island. Owing to its prominent antioxidant activity, it could be used as an ingredient in medicinal preparations, nutraceuticals, and functional foods.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Free radical scavenging activity of the peptide from the Alcalase hydrolysate of the edible aquacultural seahorse ( Hippocampus abdominalis )

Kim

Fernando

et al. 2019

J Food Biochem

View full text Add to dashboard Cite

show abstract

“…Embryos were incubated for 6 h, the embryo media were changed and the embryos developed up to 2 dpf. Intracellular ROS generation, lipid peroxidation inhibitory activity and cell death in zebrafish embryos were estimated according to the method described by Kang et al, 2013a;Kang et al, 2014;Kim et al, 2014. After incubation, the embryos were rinsed with fresh embryo media and anesthetized before observed under microscope.…”

Section: Estimation Of Intracellular Ros Generation and Lipid Peroximentioning

confidence: 99%

Protective effect of fucoxanthin isolated from Ishige okamurae against high-glucose induced oxidative stress in human umbilical vein endothelial cells and zebrafish model

Kang

Lee

et al. 2014

Journal of Functional Foods

Self Cite

View full text Add to dashboard Cite

Available online A B S T R A C THigh glucose induced oxidative stress is implicated in intracellular toxicity in tissue and blood vessel such as oxidative stress, lipid peroxidation, and cell death. In this study, we attempted to investigate protective effects of fucoxanthin isolated from Ishige okamurae against high glucose induced oxidative stress in human umbilical vein endothelial cells (HUVEC) and zebrafish model. The ROS generation, lipid peroxidation and cell death were significantly reduced in both the fucoxanthin treated HUVEC and zebrafish in vivo model, compared to those of both negative controls. This study indicates that fucoxanthin could protect cells and organ injury against oxidative stress induced by high glucose in vitro HUVEC and in vivo zebrafish model.

show abstract

“…Production of intracellular ROS in zebrafish embryos were detected using an oxidation-sensitive fluorescent probe dye, 2,7-dichlorofluorescein diacetate (DCF-DA) and generation of NO in inflammatory zebrafish model was analyzed using a fluorescent probe dye, diaminofluorophore 4-amino-5-methylamino-2′7′-difluorofluorescein diacetate (DAF-FM DA).Cell death was detected in live embryos using acridine orange staining, a nucleic acid selective metachromatic dye that interacts with DNA and RNA by intercalation or electrostatic attractions. Acridine orange staining was performed by using the method described by Kim et al (2014) with modification. At 7-9 hpf, the embryos were treated with 25, 50, and 100 μg mL -1 PRF and 1 h later, 10 μg mL -1 LPS was added to the plate.…”

Section: Estimation Of Lps-stimulated Intracellular Ros and No Producmentioning

confidence: 99%

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Kim¹,

Kim²,

Kang³

et al. 2014

ALGAE

Self Cite

View full text Add to dashboard Cite

Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. Polyphenols from E. cava have strong anti-inflammatory activity. However, a large number of the by-products from E. cava processing are discarded. In the present study, to utilize these by-products, we assessed the anti-inflammatory activity of the polyphenol-rich fraction (PRF) from E. cava processing by-product (EPB) in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells. Four compounds, namely eckol, eckstolonol, dieckol, and phlorofucofuroeckol-A, were isolated and identified from PRF. We found that PRF suppressed the production of nitric oxide (NO), inducible nitric oxide synthase, and cyclooxygenase-2 in the LPS-induced cells. Furthermore, the protective effect of PRF was investigated in vivo in LPS-stimulated inflammation zebrafish model. PRF had a protective effect against LPS-stimulated toxicity in zebrafish embryos. In addition, PRF inhibited LPS-stimulated reactive oxygen species and NO generation. According to the results, PRF isolated from EPB could be used as a beneficial anti-inflammatory agent, instead of discard.Key Words: anti-inflammation; by-product; Ecklonia cava; polyphenol; seaweeds INTRODUCTIONSeaweeds are rich in vitamins, minerals, proteins, polyphenols, and polysaccharides. Therefore, several species of seaweeds are used in cosmetics, food, and pharmaceuticals. Many studies have shown that seaweeds have diverse bioactivities such as antioxidant, anticoagulant, anti-inflammation, antibacterial, and anticancer activities (Heo et al. 2003, Shin et al. 2006, Kim et al. 2007, Erbert et al. 2012, Ko et al. 2012. In particular, Ecklonia cava is a common edible brown algae that is plentiful in Jeju Island of Republic of Korea. E. cava has been identified to contain various natural substances such as carotenoids, fucoidans, and phlorotannins, which have beneficial biological activities including antioxidant, anti-inflammation, anticoagulant, antiviral, and antidiabetic effects (Kang et al. 2010, 2012b, 2013a. Dried forms of E. cava are consumed, but steam heated E. cava products sometimes are manufactured. When steaming the alga, a large amount of by-product is produced and discarded without utilization. Some studies have suggested that fermenting Received April 25, 2014, Accepted June 3, 2014 *Corresponding Author E-mail: youjinj@jejunu.ac.kr Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Preparation of PRF from EPBEPB was kindly provided by Taerim Co., Ltd. (Jeju, Korea) and then was freeze-dried. The dried EPB powder (5 g) was suspended in 500 mL of 80% ethanol and mechanically stirred for 24 h at room temperature. The solution was filtered and the filtrate was concentrated under reduced pressure to give the oily extract. Aft...

show abstract

Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model

Cited by 101 publications

References 32 publications

Free radical scavenging activity of the peptide from the Alcalase hydrolysate of the edible aquacultural seahorse ( Hippocampus abdominalis )

Free radical scavenging activity of the peptide from the Alcalase hydrolysate of the edible aquacultural seahorse ( Hippocampus abdominalis )

Protective effect of fucoxanthin isolated from Ishige okamurae against high-glucose induced oxidative stress in human umbilical vein endothelial cells and zebrafish model

Polyphenol-rich fraction from Ecklonia cava (a brown alga) processing by-product reduces LPS-induced inflammation in vitro and in vivo in a zebrafish model

Contact Info

Product

Resources

About