Protective Effect of Galantamine Against Oxidative Damage Using Human Lymphocytes: A Novel In Vitro Model

Triana-Vidal, Luz Elena; Carvajal-Varona, Silvio

doi:10.1016/j.arcmed.2013.01.001

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…The aim of this screening is to investigate if the extract is capable of protecting neuronal cells against the oxidative stress cellular damage elicited by an oxidative stress inducer, H 2 O 2 . Even though with short half-life, H 2 O 2 is highly soluble in water and dissociates to hydroxyl and superoxide ions, which leads to oxidative damage to important macromolecules and ultimately cell death (Triana-Vidal and Carvajal-Varona, 2013 ). Thus, this molecule has been used widely in oxidative studies (Whittemore et al, 1995 ; Suematsu et al, 2011 ; Wong et al, 2012 ; Triana-Vidal and Carvajal-Varona, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

“…Even though with short half-life, H 2 O 2 is highly soluble in water and dissociates to hydroxyl and superoxide ions, which leads to oxidative damage to important macromolecules and ultimately cell death (Triana-Vidal and Carvajal-Varona, 2013 ). Thus, this molecule has been used widely in oxidative studies (Whittemore et al, 1995 ; Suematsu et al, 2011 ; Wong et al, 2012 ; Triana-Vidal and Carvajal-Varona, 2013 ). Initially, the viability of SH-SY5Y cells was examined following exposure to the extract up to 400 μg/mL and no significant toxic effect was observed in SH-SY5Y (i.e., cell viability remains >98%).…”

Section: Resultsmentioning

confidence: 99%

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Streptomyces antioxidans sp. nov., a Novel Mangrove Soil Actinobacterium with Antioxidative and Neuroprotective Potentials

et al. 2016

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A novel strain, Streptomyces antioxidans MUSC 164T was recovered from mangrove forest soil located at Tanjung Lumpur, Malaysia. The Gram-positive bacterium forms yellowish-white aerial and brilliant greenish yellow substrate mycelium on ISP 2 agar. A polyphasic approach was used to determine the taxonomy status of strain MUSC 164T. The strain showed a spectrum of phylogenetic and chemotaxonomic properties consistent with those of the members of the genus Streptomyces. The cell wall peptidoglycan was determined to contain LL-diaminopimelic acid. The predominant menaquinones were identified as MK-9(H6) and MK-9(H8), while the identified polar lipids consisted of aminolipid, diphosphatidylglycerol, glycolipid, hydroxyphosphatidylethanolamine, phospholipid, phosphatidylinositol, phosphatidylethanolamine, phosphatidylglycerol and lipid. The cell wall sugars consist of galactose, glucose and ribose. The predominant cellular fatty acids (>10.0%) were identified as iso-C15:0 (34.8%) and anteiso-C15:0(14.0%). Phylogenetic analysis identified that closely related strains for MUSC 164T as Streptomyces javensis NBRC 100777T (99.6% sequence similarity), Streptomyces yogyakartensis NBRC 100779T (99.6%) and Streptomyces violaceusniger NBRC 13459T (99.6%). The DNA–DNA relatedness values between MUSC 164T and closely related type strains ranged from 23.8 ± 0.3% to 53.1 ± 4.3%. BOX-PCR fingerprints comparison showed that MUSC 164T exhibits a unique DNA profile, with DNA G + C content determined to be 71.6 mol%. Based on the polyphasic study of MUSC 164T, it is concluded that this strain represents a novel species, for which the name Streptomyces antioxidans sp. nov. is proposed. The type strain is MUSC 164T (=DSM 101523T = MCCC 1K01590T). The extract of MUSC 164T showed potent antioxidative and neuroprotective activities against hydrogen peroxide. The chemical analysis of the extract revealed that the strain produces pyrazines and phenolic-related compounds that could explain for the observed bioactivities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Streptomyces antioxidans sp. nov., a Novel Mangrove Soil Actinobacterium with Antioxidative and Neuroprotective Potentials

et al. 2016

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“…Direct insults of H 2 O 2 have been described for oxidative stress studies [22] and neurodegenerative diseases in in vitro models, such as in the Parkinson disease model [23]. Cortical neurons were incubated for 12 h with H 2 O 2 at 10, 50, 100, 200 and 500 µM and cell viability was determined by MTT assay.…”

Section: Resultsmentioning

confidence: 99%

Spongionella Secondary Metabolites Protect Mitochondrial Function in Cortical Neurons against Oxidative Stress

et al. 2014

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The marine habitat provides a large number of structurally-diverse bioactive compounds for drug development. Marine sponges have been studied over many years and are found to be a rich source of these bioactive chemicals. This study is focused on the evaluation of the activity of six diterpene derivatives isolated from Spongionella sp. on mitochondrial function using an oxidative in vitro stress model. The test compounds include the Gracilins (A, H, K, J and L) and tetrahydroaplysulphurin-1. Compounds were co-incubated with hydrogen peroxide for 12 hours to determine their protective capacities and their effect on markers of apoptosis and Nrf2/ARE pathways was evaluated. Results conclude that Gracilins preserve neurons against oxidative damage, and that in particular, tetrahydroaplysulphurin-1 shows a complete neuroprotective activity. Oxidative stress is linked to mitochondrial dysfunction and consequently to neurodegenerative disorders like Parkinson and Alzheimer diseases, Friedreich ataxia or Amyotrophic lateral sclerosis. This neuroprotection against oxidation conditions suggest that these metabolites could be interesting lead candidates in drug development for neurodegenerative diseases.

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“…Galantamine also reduces significantly the production and release of Tumor Necrosis Factor‐α in rats with polysaccharide‐induced inflammation (Liu et al ) and can suppress the induction of iNOS, resulting in lowered NO production during hypoxia (Egea et al ). Galantamine is an efficient anti‐oxidant agent, preventing cell damage caused by hydrogen peroxide (Triana‐Vidal and Carvajal‐Varona ) or amyloid‐β (Melo et al ), this possibly being one of the mechanisms by which the drug protects against neuronal damage by β‐amyloid (Matharu et al, ; Li et al ; Rao et al ) and the toxic compounds kainic acid and caffeic acid (Kumar et al ). There is, therefor, a plethora of actions which, independently or combined, provide galantamine with a complex pharmacology which make it even more inappropriate as a tool to define the pharmacological specificity of a compound such as kynurenic acid.…”

Section: Galantamine As a Pharmacological Toolmentioning

confidence: 99%

Does kynurenic acid act on nicotinic receptors? An assessment of the evidence

Stone

2019

Journal of Neurochemistry

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As a major metabolite of kynurenine in the oxidative metabolism of tryptophan, kynurenic acid is of considerable biological and clinical importance as an endogenous antagonist of glutamate in the central nervous system. It is most active as an antagonist at receptors sensitive to N‐methyl‐D‐aspartate (NMDA) which regulate neuronal excitability and plasticity, brain development and behaviour. It is also thought to play a causative role in hypo‐glutamatergic conditions such as schizophrenia, and a protective role in several neurodegenerative disorders, notably Huntington’s disease. An additional hypothesis, that kynurenic acid could block nicotinic receptors for acetylcholine in the central nervous system has been proposed as an alternative mechanism of action of kynurenate. However, the evidence for this alternative mechanism is highly controversial, partly because at least eight earlier studies concluded that kynurenic acid blocked NMDA receptors but not nicotinic receptors and five subsequent, independent studies designed to repeat the results have failed to do so. Many studies considered to support the alternative ‘nicotinic’ hypothesis have been based on the use of analogs of kynurenate such as 7‐chloro‐kynurenic acid, or putatively nicotinic modulators such as galantamine, but a detailed analysis of the pharmacology of these compounds suggests that the results have often been misinterpreted, especially since the pharmacology of galantamine itself has been disputed. This review examines the evidence in detail, with the conclusion that there is no confirmed, reliable evidence for an antagonist activity of kynurenic acid at nicotinic receptors. Therefore, since there is overwhelming evidence for kynurenate acting at ionotropic glutamate receptors, especially NMDAR glutamate and glycine sites, with some activity at GPR35 sites and Aryl Hydrocarbon Receptors, results with kynurenic acid should be interpreted only in terms of these confirmed sites of action.

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Protective Effect of Galantamine Against Oxidative Damage Using Human Lymphocytes: A Novel In Vitro Model

Cited by 12 publications

References 23 publications

Streptomyces antioxidans sp. nov., a Novel Mangrove Soil Actinobacterium with Antioxidative and Neuroprotective Potentials

Streptomyces antioxidans sp. nov., a Novel Mangrove Soil Actinobacterium with Antioxidative and Neuroprotective Potentials

Spongionella Secondary Metabolites Protect Mitochondrial Function in Cortical Neurons against Oxidative Stress

Does kynurenic acid act on nicotinic receptors? An assessment of the evidence

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