Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-κB and p38 MAPK

Wang, Jiansheng; Su, Baishan; Zhu, Huajian; Chen, Chao; Zhang, Gang

doi:10.3892/etm.2016.3850

Cited by 71 publications

(39 citation statements)

References 31 publications

(37 reference statements)

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“…This includes their ability to reduce the levels of or the formation of ROS and RNS, concomitantly upregulating vitagenes, such as members of the heat shock protein (Hsp) family, heme oxygenase-1, and Hsp70 [8,26]. GE has been reported as an effective antioxidant and antiinflammatory agent in different experimental models from our lab and others [16][17][18][19]21,29]. Recently, the antioxidant activity of GE along with other actives has been published with respect to DPPH free radical scavenging activity and superoxide dismutase activity [30].…”

Section: Discussionmentioning

confidence: 93%

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Analysis of the Antioxidant Activity of Geraniol Employing Various in-Vitro Models: Relevance to Neurodegeneration in Diabetic Neuropathy

Prasad

Muralidhara

2017

Asian J Pharm Clin Res

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Objective: The aim of this study was to analyze antioxidant effect of geraniol (GE) in different in vitro models.Methods: Initially, the antioxidant activity of GE was assessed by diphenyl picrylhydrazyl radical (DPPH) assay. The modulatory effect of GE against 2,2’-azobis(2-amidinopropane) dihydrochloride induced lipid peroxidation in rat brain regions (cortex and cerebellum) and sciatic nerve (SN) homogenates was determined. Further, the effect of GE was assessed against hyperglycemia-induced oxidative stress (OS) in SHSY5Y, a human neuroblastoma cell line.Results: GE proved to be a good scavenger of DPPH free radical (inhibitory concentration 50% [IC50] value = 663 nmol) and could lower the lipid peroxidation levels in rat brain tissue and SN homogenates (25-40%). Further, it rescue the SHSY5Y cells from hyperglycemia-induced death. Co-exposure of GE with the IC50 level of glucose (100 mM) lowered the levels of reactive oxygen species, hydrogen peroxides and 3-nitrotyrosine levels with concomitant elevation in the glutathione levels (about two folds).Conclusion: Collectively from these findings and other studies previously conducted (from our lab and others) emphasize the potential benefit of GE against OS, a progressive pathological feature of neurodegenerative disorders.

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Section: Discussionmentioning

confidence: 93%

“…It is also found in a wide range of cleansing products and cosmetics for its appealing odor [13,14]. In the recent past, several studies have recognized the pharmacological potential of GE in different models [15][16][17]. Its pharmacological property is by virtue of its antioxidant and anti-inflammatory properties [18,19].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Antioxidant Activity of Geraniol Employing Various in-Vitro Models: Relevance to Neurodegeneration in Diabetic Neuropathy

Prasad

Muralidhara

2017

Asian J Pharm Clin Res

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“…GOH, a special type of acyclic monoterpene alcohol, has been widely used to treat many diseases related to inflammation, including dextran sulfate sodium (DSS)-induced colitis in mice [ 28 ]. Moreover, GOH has been shown to inhibit cell apoptosis in traumatic spinal cord injury (SCI) [ 29 ]. In our study, a mouse model of ALI was successfully established by the intranasal administration of LPS, and we investigated the anti-inflammatory and anti-apoptotic effects of GOH on LPS-induced ALI in mice.…”

Section: Discussionmentioning

confidence: 99%

Geraniol alleviates LPS-induced acute lung injury in mice via inhibiting inflammation and apoptosis

et al. 2017

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Geraniol (GOH), a special type of acyclic monoterpene alcohol, has been widely used to treat many diseases associated with inflammation and apoptosis. Acute lung injury (ALI) is a common clinical disease in humans characterized by pulmonary inflammation and apoptosis. In the present study, we investigated the protective effects of GOH in a mouse model of ALI induced by the intranasal administration of lipopolysaccharide (LPS) and elucidated the underlying molecular mechanisms in RAW 264.7 cells. In vivo, GOH treatment markedly ameliorated pathological injury and pulmonary cell apoptosis and reduced the wet/dry (W/D) weight ratio of lungs, myeloperoxidase (MPO) activity and the production of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). In vitro, the levels of pro-inflammatory cytokines, iNOS and COX-2 were significantly increased in LPS-stimulated RAW 264.7 cells, an effect that was decreased by GOH treatment. Moreover, GOH treatment dramatically reduced the expression of Toll-like receptor 4 (TLR4) and then prevented the nuclear factor-κB (NF-κB) activation. GOH treatment also promoted anti-apoptotic Bcl-2 expression and inhibited pro-apoptotic Bax and Caspase-3 expression. Furthermore, knockdown of TLR4 expression exerted a similar effect and inhibited the phosphorylation of p65, as well as the Bax and Caspase-3 expression. Taken together, these results suggest that GOH treatment alleviates LPS-induced ALI via inhibiting pulmonary inflammation and apoptosis, a finding that might be associated with the inhibition of TLR4-mediated NF-κB and Bcl-2/Bax signalling pathways.

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“…The Fas/FasL and Bcl-2 genes are two of the key factors influencing apoptosis and regulating the intrinsic apoptosis pathway in renal tubular epithelial cells ( 18 , 19 ). In addition, the intrinsic apoptosis pathway is activated by oxidative stress, which is mediated by increased mitochondrial membrane permeability ( 20 ). Previous studies have suggested that oxidative stress is involved in the apoptotic mechanism mediated by the Fas/FasL signaling pathway ( 21 – 23 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of apigenin on the expression levels of B‑cell lymphoma‑2, Fas and Fas ligand in renal ischemia‑reperfusion injury in rats

Liu

Wang

et al. 2017

Exp Ther Med

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The aim of the present study was to investigate the effect and possible mechanism of apigenin on renal ischemia-reperfusion (I/R) injury in rats, as well as in in vitro experiments. In total, 36 rats were subjected to 45 min of renal ischemia, with or without treatment prior to ischemia with different concentrations of apigenin (2, 10 and 50 mg/kg) administered intravenously. All rats were sacrificed at 24 h after I/R injury. The serum creatinine (Cr) and blood urea nitrogen (BUN) levels were analyzed, and histological examination was conducted. In addition, the expression levels of B-cell lymphoma 2 (Bcl-2) and Fas/Fas ligand (FasL) were detected by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blot analysis. For in vitro experiments, the NRK-52E cell line was employed. The viability, apoptosis and expression levels of Fas, FasL and Bcl-2 were examined in the culture of NRK-52E cells following the I/R. The results indicated that apigenin significantly decreased the levels of serum Cr and BUN induced by renal I/R, demonstrating an improvement in renal function. The histological evidence of renal damage associated with I/R was also mitigated by apigenin in vivo. Furthermore, apigenin increased the cell viability and decreased cell apoptosis in the culture of NRK52E after I/R in vitro. Compared with the I/R group, the expression of Bcl-2 was upregulated and the expression levels of Fas and FasL were downregulated by apigenin at the mRNA and protein levels in vivo and in vitro. In conclusion, apigenin appeared to increase the expression of Bcl-2 and reduce Fas/FasL expression in renal I/R injury, providing evident protection against renal I/R injury in rats.

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Protective effect of geraniol inhibits inflammatory response, oxidative stress and apoptosis in traumatic injury of the spinal cord through modulation of NF-κB and p38 MAPK

Cited by 71 publications

References 31 publications

Analysis of the Antioxidant Activity of Geraniol Employing Various in-Vitro Models: Relevance to Neurodegeneration in Diabetic Neuropathy

Analysis of the Antioxidant Activity of Geraniol Employing Various in-Vitro Models: Relevance to Neurodegeneration in Diabetic Neuropathy

Geraniol alleviates LPS-induced acute lung injury in mice via inhibiting inflammation and apoptosis

Effects of apigenin on the expression levels of B‑cell lymphoma‑2, Fas and Fas ligand in renal ischemia‑reperfusion injury in rats

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