Protective effect of Gnetum africanum methanol leaf extract on pancreatic islet cells in rats

Udeh, Nkeiruka E.; Anaga, Aruh O.; Asuzu, Isaac Uzoma

doi:10.1007/s00580-018-2644-2

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…Further investigations reported some preliminary data on the favorable effects of epicatechin on glycaemic homeostasis, lipid profile and systemic inflammation [30]and that consumption of epicatechin-rich green tea could lead to reduced glucose and oral testing insulin values, as well as reduced glucose and fasting insulin concentration. Studies on Gnetum africanum showed that the crude extract caused significant reductions in blood glucose levels of diabetic rats [10] and reversed alloxan-induced destruction of the pancreatic islet cells [11,31]. Its safety has also been established in Wistar rats following acute and sub-chronic studies (12).…”

Section: Discussionmentioning

confidence: 99%

“…It is relevant in ethnomedicine in Nigeria for the treatment of diabetes, piles, high blood pressure, sore throats and enlarged spleen [8,9]. Recent studies have established antidiabetic and antidyslipidaemic as well as protective effects of G. africanum on rat pancreatic islets [10][11][12]. However, no active principle has hitherto been isolated from the plant with these bioactivities.…”

Section: Introductionmentioning

confidence: 99%

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Bioactivity-guided fractionation of a methanol leaf extract from Gnetum africanum with potential anti-diabetic activity: (-)-Epicatechin as the active principle

Udeh¹,

Nnadi²,

A³

et al. 2021

jrp

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Dietary constituents of plants such as flavonoids are very important in ameliorating the challenges of metabolic disorders such as diabetes mellitus. The study evaluated the antidiabetic activities of fractions and a known flavonoid isolated from Gnetum africanum (Welw) of fasting blood sugar (FBS) in alloxan-induced diabetic albino rats. Antidiabetic activity-guided isolation by column chromatographic (CC) separation of methanol extract and purification of the most active CC fractions by semi-preparative high performance liquid chromatograpgy (HPLC) yielded a known flavonoid (GAF7.4) and four other uncharacterized fractions. The structure of GAF7.4 was elucidated based on the 1D and 2D NMR and HREIMS spectroscopic analyses. Antidiabetic activity was conducted by alloxan-induced FBS in diabetic rats model using glibenclamide as standard. The flavonoid (GAF7.4) was identified as (-)-epicatechin. The CC fraction 7 (50 mg/kg) elicited significant (p < 0.05) reduction in FBS of 42.3 % after 6 h. The isolated flavonoid (GAF7.4), 10 mg/kg dose caused a significantly higher reduction in FBS of 71.4 % in alloxan induced diabetic rats compared with 41.2 % reduction in glibenclamide (2 mg/kg) control. This represented the first report of epicatechin in G. africanum and our findings have contributed new knowledge to antidiabetic constitutents of the plants.

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