Protective Effect of Hydroalcoholic Extract of Stachys pilifera on Oxidant-Antioxidant Status in Renal Ischemia/Reperfusion Injuries in Male Rats

Abstract

Background. Renal ischemia-reperfusion (I/R) has a pivotal role in the progression of acute renal failure. Reactive oxygen species are considered the major constituents involved in the biochemical and pathophysiological changes that were shown during kidney I/R. The purpose of this study was to examine the renoprotective effects of Stachys pilifera ethanolic extract on oxidant-antioxidant status in renal I/R-injuries in male rats. Material and methods. Twenty-one male Wistar rats were arbitrarily distributed into 3 groups: sham control (SC), I/R, and I/R + Stachys pilifera ethanolic extract (500 mg/kg). The artery and vein of the right kidney were completely blocked, and the right kidney was completely removed in all groups. Then, the left kidney artery was blocked with suture thread for 30 minutes in only I/R and I/R + SP extract groups. Kidney function indices, oxidative stress markers, and hematoxylin and eosin staining were investigated in the plasma and kidney tissues. Results. It was shown that the urine Na and K, fractional excretion of Na and K, and protein carbonyl content markedly increased in the merely I/R group as compared to SC rats, while the administration of SP extract markedly reduced these indices ( P < 0.05 ). Also, glomerular filtration rate and total thiol meaningfully reduced in the I/R rats in contrast to the SC group, while the treatment with SP extract markedly augmented these indices ( P < 0.05 ). However, in agreement with renal function tests, SP extract had no significant effects on histopathological examinations. Conclusion. It seems that SP extract employs renoprotective effects on renal damage induced by I/R, possibly by improving of oxidant-antioxidant status in favor of the antioxidant system.

Glyphosate Caused Detrimental Changes in Enzymatic Antioxidants in Rats

“…Extreme evaluation of ROS gives on to construction of cytotoxic metabolites which can induce irreversible disorders like damage of DNA, Peroxidation of lipid. These changes were in agreement with the worsening of kidney function, signifying that renal I/R( Ischaemia\Reperfusion)induced kidney ROS formation, reduced the capacity of cells to get rid of ROS, augmented endogenous antioxidant depletion, and deteriorated renal injury [7]. Glyphosate (N-phosphonomethyl [glycine], is an organophosphorus compound with herbicide properties discovered in 1970.…”

Stachys pilifera Benth. Ameliorates Bleomycin-Induced Pulmonary Fibrosis in Rats through the Antioxidant Pathways

“…Oxidative stress reduces -SH reserves by oxidizing the -SH groups into disulfide or sulfonic acid. References [ 43 , 44 ]. Furthermore, the relationship between a decrease in GSH and an increase in ROS in the BLM-induced pulmonary fibrosis animal model has been confirmed [ 1 ].…”

Stachys pilifera Benth: A Review of Its Botany, Phytochemistry, Therapeutic Potential, and Toxicology

“…As reviewed herein, anti-inflammatory [ 39 ], nephroprotective [ 26 , 47 ], antimicrobial [ 32 , 33 ], antioxidant [ 26 , 32 , 33 ], hepatoprotective [ 44 , 45 ], and anticancer [ 25 ] activities of the S. pilifera were confirmed in some studies. However, there are no biological studies on the ethnomedicinal use of S. pilifera with antitussive and antiasthmatic and analgesic activities, which needs more experimental data.…”

“…Renoprotective effects of the ethanolic extract of S. pilifera against renal ischemia/reperfusion damage in rats were described by Moslemi et al, and they found that the extract (500 mg/kg) significantly reduced the renal injury in the ischemia/reperfusion model, possibly by improving some renal function indicators, restoring thiol group storage, and preventing protein oxidation. It appears that the S. pilifera extract exerts renoprotective effects against renal ischemia/reperfusion, possibly by improving the oxidant-antioxidant status [ 47 ].…”