Protective effect of hydroalcoholic extracts of Trifolium pratense L. on pancreatic β cell line (RIN-5F) against cytotoxicty of streptozotocin

Khazaei, Mozafar; Pazhouhi, Mona

doi:10.4103/1735-5362.235159

Cited by 20 publications

(9 citation statements)

References 22 publications

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“…For this reason, many studies have been conducted to evaluate the potential lipid-lowering activity of synthetic and natural compounds ( 6 ). It has been suggested that herbal remedies are among the best existing alternative therapies and have been used since ancient times ( 7 ) and they have contributed to the development of important drugs used currently in modern medicine ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

Abbas

Oriquat

Al-Najjar

et al. 2020

MJMS

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Background: Dyslipidaemias are common in patients with diabetes mellitus. A high prevalence of type 2 diabetes in hyperlipidaemic patients also exists. The aim of this study was to find a treatment that lowers both blood glucose and lipid levels simultaneously. Methods: The hypolipidaemic effect of (R)-(−)-carvone was investigated in a tyloxapol- induced hyperlipidaemia mice model. Furthermore, its effect on insulin secretion and proliferation of 1.1E7 human pancreatic β-cells was studied. In addition, using molecular docking, the binding affinity of (R)-(−)-carvone against 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG- CoA) reductase was estimated. Results: (R)-(−)-carvone (100 mg/kg) decreased plasma triglyceride, total cholesterol, low-density lipoprotein cholesterol (LDL-C) levels and atherogenic index by 90.6%, 49.3%, 56.6% and 70.3%, respectively, but it had no effect on high-density lipoprotein cholesterol (HDL-C). Furthermore, it increased hepatic triglyceride level and catalase activity by 79.6% and 59.6%, respectively. In-vitro, 500 μM (R)-(−)-carvone increased insulin secretion by 454.4% and proliferation of 1.1E7 cells with no cytotoxic effects up to a concentration of 100 μM. Molecular docking simulation demonstrated a good binding affinity with –5.03 Kcal/mol of (R)-(−)-carvone to HMG-CoA reductase. Conclusion: The hypolipidaemic effect of (R)-(−)-carvone is comparable to that of fenofibrate. (R)-(−)-carvone has the advantage over fenofibrate of not producing hypoglycaemia in animals. Furthermore, (R)-(−)-carvone increased proliferation and insulin secretion of human pancreatic β-cells.

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Section: Introductionmentioning

confidence: 99%

Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

Abbas

Oriquat

Al-Najjar

et al. 2020

MJMS

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“…The work was done in the dark, and after three times of rinsing cells with PBS, they were observed through a fluorescence microscope. 29 The apoptotic index of the cells was calculated using the following formula Apoptotic index = number of apoptotic cells/total number of cells 100×…”

Section: Tunel Stainingmentioning

confidence: 99%

Resveratrol Effect on Adipose-Derived Stem Cells Differentiation to Chondrocyte in Three-Dimensional Culture

et al. 2019

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Purpose: Adipose stem cells (ASCs) are pluripotent cells with the ability of self-renewal and differentiation into different types of mesenchymal cells. As cartilage repair is difficult due to lack of blood capillary, resveratrol (Res) is a polyphenolic compound with diverse biological properties to be possibly used in this case. The aim of the present study was to investigate the effect of Res on differentiation of ASCs into chondrocyte in a three-dimensional (3D) culture model. Methods: Subcutaneous adipose tissues were prepared and digested enzymatically, and passed through cell strainer. ASCs were harvested in the fourth passage, and divided into five groups. The control group received chondrogenic differentiation medium (CDM) while the experimental groups received CDM plus different doses of Res (1, 10, 20, and 50 µM) for 21 days. Expression of cartilage specific genes and Sirtuin1 (SIRT 1), cell viability, apoptosis and ferric reducing antioxidant power (FRAP) were detected using reverse transcription polymerase chain reaction (RT-PCR), MTT assay, TUNEL and acridine orange/ethidium bromide (AO/EB) staining. One-way ANOVA and non-parametric Mann-Whitney U test were used for data analyses. Results: ASCs were differentiated to chondrocyte by CDM in a three-dimensional culture. 10 and 20 µM doses of Res showed the most proliferating effect on ADSCs. The SIRT 1 genes expression and FRAP level also increased significantly compared to the control group (P<0.05). Also, OD of cell increased whereas apoptosis decreased. Conclusion: 3D culture was a suitable condition for ASCs differentiation to chondrocyte, and lower doses of Res exert proliferation effect on ASCs.

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“…This disease is the most common chronic disorder in the whole world. It is estimated that by 2030, the number of people suffering from the disease will reach more than 370 million ( 3 ). Increased generation of reactive oxygen species and the occurrence of oxidative stress led to the destruction of insulin-secreting beta cells of the islets of Langerhans.…”

Section: Introductionmentioning

confidence: 99%

Hypoglycemic, hepatoprotective, and hypolipidemic effects of hydroalcoholic extract of Eryngium billardieri root on nicotinamide/streptozotocin-induced type II diabetic rats

Khani

Abdollahi

Asadi

et al. 2021

Research in Pharmaceutical Sciences

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Background and purpose: Erynginum billardieri has been used to control diabetes in traditional medicine. This research was performed to study the antidiabetic, hepatoprotective, and hypolipidemic effects of E. billardieri root extract (EBRE) on streptozotocin/nicotinamide-induced type 2 diabetic male rats. Experimental approach: Type two diabetic animals were treated by three different doses of EBRE (100, 200, and 400 mg/kg), orally administered for 4 weeks. Ultimately, after anesthesia, the glucose, insulin, lipid profiles, hepatic enzyme levels in the blood and liver, and pancreas tissues of the animals were analyzed. Findings/Results: Induction of diabetes caused a diminution in insulin level, high-density lipoprotein (HDL), and significantly enhanced the level of other lipid profiles, glucose, and liver enzymes ( P < 0.05). Administration of the EBRE to diabetic-male rats significantly reduced glucose level, lipid profiles, and liver enzymes, and increased the level of HDL to near normal. Conclusion and implications: The results of the present study showed that E. billardieri had a positive effect on diminishing the lipid profiles, liver enzymes, and controlling diabetes. The most effective dose was found to be 100 mg/kg.

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Protective effect of hydroalcoholic extracts of Trifolium pratense L. on pancreatic β cell line (RIN-5F) against cytotoxicty of streptozotocin

Cited by 20 publications

References 22 publications

Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone

Resveratrol Effect on Adipose-Derived Stem Cells Differentiation to Chondrocyte in Three-Dimensional Culture

Hypoglycemic, hepatoprotective, and hypolipidemic effects of hydroalcoholic extract of Eryngium billardieri root on nicotinamide/streptozotocin-induced type II diabetic rats

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