Protective Effect ofAmphipterygium adstringensExtract on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice

Abstract

Amphipterygium adstringens is an endemic species in Mexico commonly known as “cuachalalate.” Healers to treat gastritis, gastric ulcers, and gastrointestinal cancer have traditionally used the bark. We investigated the effects of alcoholic extract of A. adstringens (AaEE) in DSS-induced colitis in mice. The protective effect of AaEE was determined at 200 mg/kg by oral gavage for 10 days. We determine the effect of AaEE on clinical features (disease activity index), antioxidants, anti-inflammatory, and immunomodulatory activities in relation to the activity of SOD, CAT, and GPx, levels of proinflammatory cytokines, and changes both macroscopic and microscopic of the colonic mucosa. AaEE significantly reduced the inflammation of colon and significantly increased SOD and GPx activities. AaEE also significantly decreased TNF-α, IFN-γ, and IL-1β cytokine levels compared to DSS-treated mice and reduced both infiltration of inflammatory cells and the mucosal damage in colon. The results suggested the protective potential of AaEE in DSS-induced colitis and this might be attributed to its phytochemicals compounds that have been found to induce a wide spectrum of activities such as reduction in oxidative stress, suppression of inflammation, modulating numerous signal transduction pathways, and induction of apoptosis. The findings of this study suggest that AaEE has substantial potential for the treatment of inflammatory colitis.

Anti-Inflammatory and Antioxidant Activities of the Methanolic Extract of Cyrtocarpa procera Bark Reduces the Severity of Ulcerative Colitis in a Chemically Induced Colitis Model

“…Because A. adstringens and C. procera are members of the same family, Anacardiaceae, and due to their commercial presentation, these, like many other plants sold in markets, are liable to adulteration [14]. In this work, we demonstrated that the biomedical and therapeutic properties that the C. procera methanolic extract exerted in this DSS-induced colitis model are very similar to those of A. adstringens, which was studied previously by our work team [4]. Both C. procera and A. adstringens extracts had remarkable antioxidant and anti-inflammatory effects and greatly diminished UC severity.…”

“…The electrondonating capacity of the extract was calculated from the bleaching of the purple-colored DPPH solution dissolved in methanol. Ninety-six-well ELISA plates were filled with extract concentrations ranging from 1 to 100 μg/mL and 100 μM DPPH solution [4]. Quercetin was used as a control, and the same concentrations of the extract were used.…”

“…Ulcerative colitis (UC) is an inflammatory disease limited to the colonic mucosa [1,2] that is characterized by a variety of symptoms, including abdominal pain and cramping, bloody diarrhea, rectal bleeding, weight loss, fever, and fatigue, which may begin gradually or start all at once [3,4]. Currently, the pathogenesis of UC remains unknown, but it has been related to multifactorial mechanisms involving interactions between genetic and immunological and environmental factors [5].…”

“…A. adstringens is also used in Mexican traditional medicine to treat different gastrointestinal diseases, such as gastritis, gastric ulcers, and gastrointestinal cancer [4,13]. Moreover, our recent work demonstrated that the methanolic extract of the bark of A. adstringens decreases UC severity by reducing both inflammation and oxidative stress [4], two of the most important factors in UC establishment and progression [16].…”

Berberrubine attenuates mucosal lesions and inflammation in dextran sodium sulfate-induced colitis in mice

“…Inflammation responses are one of the most crucial factors causing UC [ 3 ]. The increased pro-inflammatory cytokines such as TNF-α, IFN-γ, and IL-1β, extend the inflammatory cascade and eventually lead to intestinal/colonic tissue damage in UC induced by DSS [ 4 ].…”