Protective Effect of Coptis chinensis Polysaccharide Against Renal Injury by Suppressing Oxidative Stress and Inflammation in Diabetic Rats

Abstract

Our previous studies confirmed that Coptis chinensis polysaccharide (CCPW) had a good antidiabetic activity and could improve insulin resistance. However, whether CCPW has a protective effect against the renal injury caused by diabetes has not been reported. In this study, the protective effect of CCPW against the renal injury of diabetic rats and its underlying mechanisms were investigated. The results showed that in CCPW-treated groups, the body mass of rats increased significantly, while the ratio of kidney weight to body weight decreased significantly; the 24-hour urine protein and the serum BUN and serum creatinine (Scr) levels decreased significantly, and pathological changes of the renal tissue were improved; superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities in the renal tissue were significantly higher, and malondialdehyde (MDA) contents in the renal tissue, and tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and hypersensitive c-reactive protein (hs-CRP) levels in the serum were significantly lower; the expressions of phosphorylated tyrosine kinase 2 (p-JAK2) and activators of transcription 3 (p-STAT3) decreased significantly. The results indicate that CCPW should have a protective effect against the renal injury in diabetic rats, which may be associated with its inhibition on oxidative stress and inflammation.

Nephro‐protective effect of Daphnetin in hyperoxaluria‐induced rat renal injury via alterations of the gut microbiota

“…The inflammatory reaction plays a crucial role in the initiation and progression of diabetic nephropathy (Jiang et al, 2019). Previous research report that the expansion of the hyperoxaluria condition through secretion of various types of inflammatory cytokines (Ozturk et al, 2019).…”

“…The secretion of different inflammatory cytokines by activated macrophages contributes to the progression of diabetic nephropathy (Cawthorn & Sethi, 1998; Chen et al, 2017). IL‐6 is a cytokine produced by vascular endothelial cells, activated monocytes and fibroblasts that has a wide range of biological effects and helps to facilitate biological processes such as tissue injury, tissue repair, host defense, and inflammatory response by affecting humoral and cellular immunity functions (Jiang et al, 2019). IL‐6 is a key inflammatory factor in the production of acute inflammation and the manufacture of CRP, as well as a key inflammatory component in the relationship between local vascular injury and the systemic immune response (Arik et al, 2013).…”

“…Daphnetin effectively reduced the proliferation of reactive oxygen scavengers in renal tubular cells while preventing LPO levels. The inflammatory reaction plays a crucial role in the initiation and progression of diabetic nephropathy (Jiang et al, 2019).…”

“…IL‐6 can increase the activation of B and T cells, as well as mesangial proliferation and CRP production, when hyperglycemia is present. Continuous CRP production can increase the monocyte adherence to endothelial cells, inducing the direct injury to vascular endothelium cells and increasing the vascular injury (Jiang et al, 2019). The Protective effect of pentoxifylline on oxidative renal cell injury linked with the renal crystal formation in a hyperoxaluric rats which showed the higher levels of IL‐1β, MCP‐1, IL‐6, and TNF‐α, suggesting a link between the renal dysfunction and inflammation.…”

A dataset of branched fatty acid esters of hydroxy fatty acids diversity in foods

“…We profiled the FAHFAs from 12 food samples, including 3 algae (kelp, Spirulina, and Nostoc commune Vauch), 1 fungus (Lentinus edodes), 6 plant foods (tomato, apple, peanut, black sesame, wheat grains, and rice grains), and 2 animal foods (egg and fish). Additionally, 4 medicinal food samples (lotus plumule, Chinese yam, Lycium chinense, and Coptis chinensis) were also selected for FAHFA profiling because they have similar properties (anti-type 2 diabetes or anti-inflammatory) to FAHFA and are commonly consumed in daily life [33][34][35][36] . By using the CIL-LC-MRM MS method, a total of 1207 regioisomers belonging to 298 FAHFA families were detected from the above 12 food samples and 4 medicinal food samples (Table S4).…”