Protective Effect ofOenanthe javanicaon the Hepatic Lipid Peroxidation in Bromobenzene-Treated Rats and its Bioactive Component

Abstract

To investigate the detoxification of bromobenzene-induced hepatic lipid peroxidation by Oenanthe javanica DC, the hepatic lipid peroxide level and the activities of enzymes responsible for production and removal of epoxide were studied. The level of lipid peroxide elevated by bromobenzene was significantly reduced by the methanol extract (250 mg/kg) and persicarin (5 mg/kg). The methanol extract and persicarin administered daily over 4 weeks before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase, and glutathione S-transferase. Epoxide hydrolase activity was decreased significantly by bromobenzene, which was restored to the control level by pretreatment with persicarin. However, the identical pretreatment with isorhamnetin and hyperoside did not change the enzyme activity or lipid peroxide level. The results suggest that the reduction of bromobenzene-induced hepatic lipid peroxidation by O. javanica under our experimental conditions is effected through enhancing the activity of epoxide hydrolase, an enzyme removing bromobenzene epoxide. In addition, the bioactive component of this plant responsible for the detoxification of bromobenzene, at least in part, is thought to be persicarin.

In vitro and in vivo anti-inflammatory activities of Polygonum hydropiper methanol extract

“…Several components of this plant have been reported to display various biological activities. For example, anti-fungal (polygodial and warburganal), anti-inflammatory (polygonolide), and anti-oxidative (hydropiperoside, rhamnazin, and persicarin) compounds were reported previously (Ley, 1990;Park et al, 1996;Takasaki et al, 2001;Peng et al, 2003;Kiem et al, 2008).…”

Effects of Bergenin, the Major Constituent of <i>Mallotus japonicus</i> against <i>D</i>-Galactosamine-Induced Hepatotoxicity in Rats

“…The rats were anesthetized with CO 2 gas, and blood was collected from the abdominal aorta [10,11]. It was centrifuged at 3,000 rpm for 15 min to separate the serum and kept at 4°C to assess the serum enzymes activities.…”

Persicarin isolated from Oenanthe javanica protects against diabetes-induced oxidative stress and inflammation in the liver of streptozotocin-induced type 1 diabetic mice

“…O. javanica , which contains high levels of vitamins and minerals, is consumed as a vegetable, and has also been used as a medicinal agent. It has a long history of use for the treatment of inflammatory conditions, including hepatitis (8,9). Hyperoside, persicarin and isorhamnetin are the three major substances with pharmacological activities that have been identified in O. javanica .…”