Protective Effect of <i>Paulownia tomentosa</i> Fruits in an Experimental Animal Model of Acute Lung Injury

Kim, Seong‐Man; Ryu, Hyung Won; Kwon, Ok‐Kyoung; Min, Jeong Ki; Park, Jin-Mi; Kim, Doo-Young; Oh, Sei‐Ryang; Lee, Seung Jin; Ahn, Ki Hoon; Lee, Jae Won

doi:10.48022/mbl.2112.12007

Cited by 4 publications

(4 citation statements)

References 28 publications

(35 reference statements)

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“…The upregulation of various molecules, including cytokines, chemokines, and adhesion molecules, has been reported in the pathogenesis of asthma [ 36 , 51 , 52 ] and in experimental models of inflammatory lung diseases, including AA [ 53 , 54 , 55 ]. Accumulated evidence has indicated that the activation of NF-κB plays a pivotal role in the expression of inflammatory molecules in lung epithelial cells and macrophages [ 5 , 13 , 15 , 56 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma

Park

Choi

Lee

et al. 2022

IJMS

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Methyl p-coumarate (methyl p-hydroxycinnamate) (MH) is a natural compound found in a variety of plants. In the present study, we evaluated the ameliorative effects of MH on airway inflammation in an experimental model of allergic asthma (AA). In this in vitro study, MH was found to exert anti-inflammatory activity on PMA-stimulated A549 airway epithelial cells by suppressing the secretion of IL-6, IL-8, MCP-1, and ICAM-1. In addition, MH exerted an inhibitory effect not only on NF-κB (p-NF-κB and p-IκB) and AP-1 (p-c-Fos and p-c-Jun) activation but also on A549 cell and EOL-1 cell (eosinophil cell lines) adhesion. In LPS-stimulated RAW264.7 macrophages, MH had an inhibitory effect on TNF-α, IL-1β, IL-6, and MCP-1. The results from in vivo study revealed that the increases in eosinophils/Th2 cytokines/MCP-1 in the bronchoalveolar lavage fluid (BALF) and IgE in the serum of OVA-induced mice with AA were effectively inhibited by MH administration. MH also exerted a reductive effect on the immune cell influx, mucus secretion, and iNOS/COX-2 expression in the lungs of mice with AA. The effects of MH were accompanied by the inactivation of NF-κB. Collectively, the findings of the present study indicated that MH attenuates airway inflammation in mice with AA, suggesting its potential as an adjuvant in asthma therapy.

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Section: Discussionmentioning

confidence: 99%

Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma

Park

Choi

Lee

et al. 2022

IJMS

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“…Considering that the MAPK/NF-κB signaling pathways have been admitted as an important target in ALI therapy [26,27], the present study selected these signaling pathways to examine the underlying mechanisms. It was confirmed that FM could suppress the LPS-induced activation of JNK/p38/ERK in in vitro experiments.…”

Section: Discussionmentioning

confidence: 99%

Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages

Park

Eum

et al. 2022

Microbiol. Biotechnol. Lett.

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Ficus vasculosa Wall. ex Miq. (FV) has been used as a herbal medicine in Southeast Asia and its antioxidant activity has been shown in previous studies. However, it has not yet been elucidated whether FV exerts anti-inflammatory effects on activated-macrophages. Thus, we aimed to evaluate the ameliorative property of FV methanol extract (FM) on lipopolysaccharide (LPS)-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 macrophages. The experimental results indicated that FM decreased the production of inflammatory mediators (NO/PGE2) and the mRNA/protein expression of iNOS and COX-2 in LPS-stimulated RAW264.7 cells. FM also reduced the secretion of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 in LPS-stimulated RAW264.7 cells. Results also demonstrated that FM improved inflammatory response in LPS-stimulated A549 airway epithelial cells by inhibiting the production of cytokines, such as IL-1β, IL-6 and TNF-α. In addition, FM suppressed MAPK activation and NF-κB nuclear translocation induced by LPS. FM also upregulated the mRNA/protein expression levels of heme oxygenase-1 and the nuclear translocation of nuclear factor erythroid 2-related factor 2 in RAW264.7 cells. In an experimental animal model of LPSinduced acute lung injury, the increased levels of molecules in bronchoalveolar lavage (BAL) fluid were suppressed by FM administration. Collectively, it was founded that FM has anti-inflammatory properties on activated-macrophages by suppressing inflammatory molecules and regulating the activation of MAPK/ NF-κB signaling.

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“…Briefly, mice were administered a mixture of OVA and alum (day 0: 30 µg OVA and 3 mg alum; day 7: 60 µg OVA and 3 mg alum). On days 11-13, mice were administered OVA through inhalation daily for 1 h. On days 9-13, mice were orally administered BGE or dexamethasone (DEX); treatment with 1 mg/kg of DEX is widely used as a positive control for asthma treatment [53][54][55].…”

Section: Animal Experimentsmentioning

confidence: 99%

“…; Bayer Korea, Ltd., Seoul, Republic of Korea) by intraperitoneal injection. On day 15, BALF and serum were collected using a previously reported method [53]. The levels of Th2 cytokines in BALF (R&D systems, Minneapolis, MN, USA) and IgE in serum (Biolegend, San Diego, CA, USA, # 439807) were measured by enzymelinked immunosorbent assay (ELISA) kits, following the manufacturer's instructions.…”

Section: Analysis Of Bronchoalveolar Lavage Fluid (Balf) Of An Animal...mentioning

confidence: 99%

Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway

Song

Lee

Ryu

et al. 2023

IJMS

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Asthma is a chronic inflammatory lung disease that causes respiratory difficulties. Black ginseng extract (BGE) has preventative effects on respiratory inflammatory diseases such as asthma. However, the pharmacological mechanisms behind the anti-asthmatic activity of BGE remain unknown. To investigate the anti-asthmatic mechanism of BGE, phorbol 12-myristate 13-acetate plus ionomycin (PMA/Iono)-stimulated mouse EL4 cells and ovalbumin (OVA)-induced mice with allergic airway inflammation were used. Immune cells (eosinophils/macrophages), interleukin (IL)-4, -5, -13, and serum immunoglobulin E (IgE) levels were measured using an enzyme-linked immunosorbent assay. Inflammatory cell recruitment and mucus secretion in the lung tissue were estimated. Protein expression was analyzed via Western blotting, including that of inducible nitric oxide synthase (iNOS) and the activation of protein kinase C theta (PKCθ) and its downstream signaling molecules. BGE decreased T helper (Th)2 cytokines, serum IgE, mucus secretion, and iNOS expression in mice with allergic airway inflammation, thereby providing a protective effect. Moreover, BGE and its major ginsenosides inhibited the production of Th2 cytokines in PMA/Iono-stimulated EL4 cells. In EL4 cells, these outcomes were accompanied by the inactivation of PKCθ and its downstream transcription factors, such as nuclear factor of activated T cells (NFAT), nuclear factor kappa B (NF-κB), activator of transcription 6 (STAT6), and GATA binding protein 3 (GATA3), which are involved in allergic airway inflammation. BGE also inhibited the activation of PKCθ and the abovementioned transcriptional factors in the lung tissue of mice with allergic airway inflammation. These results highlight the potential of BGE as a useful therapeutic and preventative agent for allergic airway inflammatory diseases such as allergic asthma.

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Protective Effect of Paulownia tomentosa Fruits in an Experimental Animal Model of Acute Lung Injury

Cited by 4 publications

References 28 publications

Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma

Methyl P-Coumarate Ameliorates the Inflammatory Response in Activated-Airway Epithelial Cells and Mice with Allergic Asthma

Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages

Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway

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