Protective Effect ofThunbergia laurifolia(Linn.) on Lead Induced Acetylcholinesterase Dysfunction and Cognitive Impairment in Mice

Abstract

Thunbergia laurifolia (linn., TL), a natural phenolic compound, has been reported to have many benefits and medicinal properties. The current study ascertains the total phenolic content present in TL aqueous leaf extract and also examines the antioxidant ability of the extract in preserving acetylcholinesterase (AChE) activity of mice exposed to lead in vivo and in vitro model. Mice were given lead acetate (Pb) in drinking water (1 g/L) together with TL 100 and 200 mg/kg/day. The result showed that Pb induced AChE dysfunction in both in vitro and in vivo studies. TL significantly prevented Pb induced neurotoxicity in a dose-dependent manner which was indicated by comparatively better performance of TL treated mice in Morris Water Maze Swimming Test and increased AChE activity in the tissue sample collected from the brains of these mice. TL also exhibited the greatest amount of phenolic content, which has a significant positive correlation with its antioxidant capacity (P < 0.05). Taken together, these data suggested that the total phenolic compounds in TL could exhibit antioxidant and in part neuroprotective properties. It may play a potential treatment strategy for Pb contamination.

Long-term changes in brain cholinergic system and behavior in rats following gestational exposure to lead: protective effect of calcium supplement

“…Another study by Reddy et al, ( 2007 ) reported sensitivity of the cholinergic system to Pb, supported by in vitro studies of 35 PND rat brains using different concentrations of Pb, which showed dose dependent inhibitory actions on the cholinergic system. However, previous reports found that Pb exposure had a direct action on cholinergic neurons causing decrease of AChE coupled with increased ACh (Mansouri et al, 2013 ; Phyu & Tangpong, 2013 ). Pb has a high affinity to free SH groups of AChE and its binding can alter their function, reflecting a possible increase in ACh levels in brain tissues, which is consistent with earlier reports (Reddy et al, 2007 ; Mansouri et al, 2013 ; Basha et al, 2014 ).…”

Neuropharmacological Potential and Delivery Prospects of Thymoquinone for Neurological Disorders

“…Lead (Pb) is known as a highly neurotoxic agent that causes functional and structural abnormalities in the brains [ 131 ]. In addition, Pb induces neurotoxicity in the cerebrum, cerebellum, and medulla oblongata of rat brain.…”

Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract

“…1 It has been widely used as detoxification and antipyretic drug in Thai traditional medicine for centuries 2 and also included in "Thailand National List of Essential Medicines". 3 Modern pharmacological experiments revealed that the TL possessed antioxidant, 4 antiinflammatory, 5 hepatoprotective, 6,7 antidote, 8,9 antitumor 10 and anti-hyperglycemic activities. 11 Previous studies of TL reported that rosmarinic acid (RA) is the major anti-oxidative constituent 12 together with its derivatives caffeic acid (CA).…”