Protective effect of indigo naturalis extract against oxidative stress in cultured human keratinocytes

Lin, Yin-Ku; Chen, Hsiao‐Wen; Yang, Sien-Hung; Leu, Yann‐Lii; Huang, Yu-Hsiang; Yen, Hsiu-Chuan

doi:10.1016/j.jep.2011.12.037

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…Indigo naturalis has been found to exert anti-inflammatory effect by reducing the oxidative stress of epidermal keratinocytes and neutrophils [30,31]. Indigo naturalis has also been shown to exert anti-inflammatory effect by inhibiting the adhesion of lymphocytes and reducing the c-jun activation and VCAM-1 gene expression of human vascular endothelial cells [30]. These results might contribute significantly to the reduced infiltration of lymphocytes in psoriatic lesions after the clinical treatment with indigo naturalis [32].…”

Section: Discussionmentioning

confidence: 99%

“…It is possible that indigo naturalis might exert additional effects that prevent the unwanted inflammation-related consequences. Indigo naturalis has been found to exert anti-inflammatory effect by reducing the oxidative stress of epidermal keratinocytes and neutrophils [30,31]. Indigo naturalis has also been shown to exert anti-inflammatory effect by inhibiting the adhesion of lymphocytes and reducing the c-jun activation and VCAM-1 gene expression of human vascular endothelial cells [30].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes

Hsieh¹,

Lin²,

Tsai³

et al. 2012

Journal of Dermatological Science

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes

Hsieh¹,

Lin²,

Tsai³

et al. 2012

Journal of Dermatological Science

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“…Decoction is the main form of traditional Chinese medicine [28]. Yin-Ku Lin et al found that the abstracts of Indigo naturalis in oil reduced the Nail Psoriasis Severity Index in patient, and the abstracts of Indigo naturalis in dimethyl sulfoxide inhibited suppressed the increase of protein carbonyl groups in human keratinocytes [29,30]. However, as the main therapeutic dosage form of traditional Chinese medicine, rare research regarding the decoction of indigo naturalis has been seen on the acute lung injury induced by in uenza A virus.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effect of Indigo Naturalis on Acute Lung Injury Induced by Influenza a Virus

Tian

et al. 2020

Preprint

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Background: Infections induced by influenza viruses, as well as COVID-19 pandemic induced by SARS-CoV-2 led to Acute lung injury (ALI) and multiorgan failure, during which traditional Chinese medicine played an important role in treatment of the pandemic. The study aimed to investigate the effect of indigo naturalis on ALI induced by influenza A virus (IAV) in mice.Method: The anti-influenza and anti-inflammatory properties of aqueous extracts of indigo naturalis (INAE) were evaluated in vitro. BALB/c mice inoculated intranasally with IAV (H1N1) were treated intragastrically with INAE (40, 80 and 160 mg kg-1/d) 2 h later for 4 or 7 days. Animal mortality and lifespan were recorded. Expression of high mobility group box-1 protein (HMGB-1) and toll-like receptor-4 (TLR4) were evaluated through immunohistological staining. Inflammatory cytokines were also monitored by ELISA.Result: INAE inhibited virus growth on Madin-Darby canine kidney (MDCK) cells and decreased nitric oxide (NO) production from lipopolysaccharide (LPS)-stimulated peritoneal macrophage in vitro. The results showed that oral administration of 160 mg/kg of INAE significantly improved the lifespan (P < 0.01) and survival rate of IAV infected mice, improved lung injury and lowered viral replication in lung tissue (P < 0.01). Treatment with INAE (40, 80 and 160 mg/kg) also significantly increased liver weight and liver index (P < 0.05), as well as spleen and thymus weight and organ index at 160 mg/kg (P < 0.05). The expression of HMGB-1 and TLR4 in lung tissue were also suppressed. Treatment with INAE reduced the high levels of interferon α (IFN-α), interferon β (IFN-β), interferon γ (IFN-γ), monocyte chemoattractant protein-1 (MCP-1), regulated upon activation normal T cell expressed and secreted factor (RANTES), interferon induced protein-10 (IP-10), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) (P < 0.05), with increased production of interleukin-10 (IL-10) (P < 0.05). The increased myeloperoxidase (MPO) activity and methylene dioxyamphetamine (MDA) level in lung tissues were inhibited by INAE treatment (P < 0.05).Conclusion: The results showed that INae alleviated IAV induced ALI in mice. The effect of INAE might be related with its anti-virus, anti-inflammatory and anti-oxidation properties, which give a hint that indigo naturalis might be effective on respiratory viruses infected acute lung injury or SAR-CoV-2 caused COVID-19.

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“…It has previously been shown that indirubin is a potent inhibitor of wide range of kinases, but, in particular, it strongly suppresses the activation of cyclin-dependent kinases (16). Herbal extracts containing indigo or its derivatives also have been shown to have potent antibacterial (17), antitumor (18), anti-inflammatory (19) and antioxidant (20) activities. Accordingly, PR is a promising candidate for the treatment of periodontal diseases.…”

Section: Inhibitory Effects Of Persicariae Rhizoma Aqueous Extracts Omentioning

confidence: 99%

Inhibitory effects of Persicariae Rhizoma aqueous extracts on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats

Kang

Lee

Han

et al. 2016

Experimental and Therapeutic Medicine

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Persicariae Rhizoma (PR) is the dried stem parts of H. Gross (Polygonaceae), and has been traditionally used as anti-inflammatory and detoxifying agent. In the present study, the effects of PR aqueous extracts on ligation-induced experimental periodontitis (EPD) and associated alveolar bone loss in rats were examined. Following the induction of EPD in rats, PR extracts were orally administered once a day for 10 days, and the changes and gains in body weight, alveolar bone loss and total aerobic bacterial counts of buccal gingiva were observed with histopathological analysis. In addition, anti-inflammatory effects were evaluated by monitoring myeloperoxidase (MPO) activities, and interleukin (IL)-1β and tumor necrosis factor (TNF)-α contents, and anti-oxidant effects were investigated by measuring inducible nitric oxide synthase (iNOS) activities and malondialdehyde (MDA) levels. Bacterial proliferation, periodontitis and associated alveolar bone loss induced by ligature placement were significantly and dose-dependently inhibited by the treatment with PR extracts. The inhibitory effects of 200 mg/kg PR were similar to those of 5 mg/kg indomethacin on ligation-induced periodontitis and associated alveolar bone losses in this study. The results suggest that PR effectively inhibits ligature placement-induced periodontitis and alveolar bone loss in rats via antibacterial, antioxidative and anti-inflammatory activities.

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Protective effect of indigo naturalis extract against oxidative stress in cultured human keratinocytes

Cited by 17 publications

References 14 publications

Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes

Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes

Beneficial Effect of Indigo Naturalis on Acute Lung Injury Induced by Influenza a Virus

Inhibitory effects of Persicariae Rhizoma aqueous extracts on experimental periodontitis and alveolar bone loss in Sprague-Dawley rats

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