Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

Taşdemir, Cemal; Tasdemir, Seda; Vardı, Nigar; Ateş, Burhan; Parlakpınar, Hakan; Kati, Bülent; Karaaslan, Merve Gökşin; Acet, Ahmet

doi:10.3109/0886022x.2012.717490

Cited by 37 publications

(32 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, a comprehensive understanding of this process is mandatory to improve intervention strategies [33]. After transplantation, IRI is clinically important because it can cause acute organ failure and high patient mortality [34,35]. ROS and proinflammatory cytokines which occur during BD as well as CIT play a key role in the pathophysiology of IRI [35].…”

Section: Discussionmentioning

confidence: 99%

“…After transplantation, IRI is clinically important because it can cause acute organ failure and high patient mortality [34,35]. ROS and proinflammatory cytokines which occur during BD as well as CIT play a key role in the pathophysiology of IRI [35]. In order to successfully target ROS, it is necessary to consider the specific ROS involved, the sources generating ROS in what particular location, at what time in the pathogenesis, and how much oxidative stress is generated.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

et al. 2013

View full text Add to dashboard Cite

BackgroundAs organ shortage is increasing, the acceptance of marginal donors increases, which might result in poor organ function and patient survival. Mostly, organ damage is caused during brain death (BD), cold ischemic time (CIT) or after reperfusion due to oxidative stress or the induction of apoptosis. The aim of this study was to study a panel of genes involved in oxidative stress and apoptosis and compare these findings with immunohistochemistry from a BD and living donation (LD) pig model and after cold ischemia time (CIT).MethodsBD was induced in pigs; after 12 h organ retrieval was performed; heart, liver and kidney tissue specimens were collected in the BD (n = 6) and in a LD model (n = 6). PCR analysis for NFKB1, GSS, SOD2, PPAR-alpha, OXSR1, BAX, BCL2L1, and HSP 70.2 was performed and immunohistochemistry used to show apoptosis and nitrosative stress induced cell damage.ResultsIn heart tissue of BD BAX, BCL2L1 and HSP 70.2 increased significantly after CIT. Only SOD2 was over-expressed after CIT in BD liver tissue. In kidney tissue, BCL2L1, NFKB, OXSR1, SOD2 and HSP 70.2 expression was significantly elevated in LD. Immunohistochemistry showed a significant increase in activated Caspase 3 and nitrotyrosine positive cells after CIT in BD in liver and in kidney tissue but not in heart tissue.ConclusionThe up-regulation of protective and apoptotic genes seems to be divergent in the different organs in the BD and LD setting; however, immunohistochemistry revealed more apoptotic and nitrotyrosine positive cells in the BD setting in liver and kidney tissue whereas in heart tissue both BD and LD showed an increase.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Both right nephrectomy model and duration of renal I/R were selected according to our previous renal I/R studies in rat. 20,21 Sham-operated (first group) and sitagliptin-administrated group (third group) animals only underwent right nephrectomy. I/R groups (second and fourth groups) both underwent right nephrectomy and I/R procedure.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Protective effect of sitagliptin against renal ischemia reperfusion injury in rats

Nuransoy¹,

Beytur²,

Polat

et al. 2015

Renal Failure

Self Cite

View full text Add to dashboard Cite

This study was designed to investigate the protective effects of sitagliptin on renal damage induced by renal ischemia reperfusion (I/R) in rats. For this, rats were randomly divided into four groups (n ¼ 8): (1) sham group, in which the rats only underwent right nephrectomy; (2) right nephrectomy and left kidney ischemia (1 h) and reperfusion (24 h) group (I/R); (3) 5 mg/kg sitagliptin administrated group, per-oral once a day for two weeks; (4) 5 mg/kg sitagliptin administrated group, per-oral once a day for two weeks before left kidney I/R (n ¼ 8). Sitagliptin-treated rats that underwent renal I/R demonstrated significant decrease in the serum urea nitrogen and creatinine and also, lipid peroxidation, total oxidant status and malondialdehyde level in the renal tissue when compared to the renal I/R group. Additionally, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase and total antioxidative capacity were significantly increased after renal I/R in sitagliptin-treated rats. Our histopathological findings were in accordance with these biochemical results. In sum, in the current study all of our results indicated that sitagliptin treatment ameliorated renal damage induced by renal I/R in rats.

show abstract

“…High TNF- α increases ROS, causing increased apoptosis [41]. It has been demonstrated by some studies that a prophylactic anti-TNF- α treatment, such as infliximab, may be an effective therapeutic strategy for preventing I/R-induced injury [18, 42, 43]. ADA is a potent antibody against TNF- α , which can neutralize all forms (extracellular, transmembrane, and receptor-bound) of TNF- α .…”

Section: Discussionmentioning

confidence: 99%

Adalimumab Ameliorates Abdominal Aorta Cross Clamping Which Induced Liver Injury in Rats

Cüre

Tümkaya

Kalkan

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

The aim of this study was to investigate the possible protective effects of adalimumab (ADA) on cell damage in rat liver tissue during ischemia/reperfusion (I/R) injury of infrarenal abdominal aorta. Thirty male Wistar-albino rats were divided into three groups: control, I/R, and I/R+ADA, each group containing 10 animals. Laparotomy without I/R injury was performed in the control group animals. Laparotomy in the I/R group was followed by two hours of infrarenal abdominal aortic cross ligation and then two hours of reperfusion. ADA (50 mg/kg) was administered intraperitoneally as a single dose, to the I/R+ADA group, five days before I/R. The tumor necrosis factor-alpha (TNF-α) (pg/mg protein) and nitric oxide (NO) (µmol/g protein) levels in the I/R group (430.8 ± 70.1, 8.0 ± 1.1, resp.) were significantly higher than those in the I/R+ADA group (338.0 ± 71.6, P = 0.006; 6.3 ± 1.2, P = 0.008) and the control group (345.5 ± 53.3, P = 0.008; 6.5 ± 1.5, P = 0.010, resp.). I/R causes severe histopathological injury to the liver tissue, but ADA leads to much less histopathological changes. ADA treatment significantly decreased the severity of liver I/R injury. ADA pretreatment may have protective effects on experimental liver injury.

show abstract

Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney

Cited by 37 publications

References 33 publications

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

Oxidative stress and apoptosis in a pig model of brain death (BD) and living donation (LD)

Protective effect of sitagliptin against renal ischemia reperfusion injury in rats

Adalimumab Ameliorates Abdominal Aorta Cross Clamping Which Induced Liver Injury in Rats

Contact Info

Product

Resources

About