Protective effect of isoflavone enriched soy β ‐conglycinin on osteoporosis in ovariectomized rats

Abstract

Research shows that the consumption of soybean foods can reduce the incidence rate of bone fractures in women after menopause. The aim of this study was to investigate the effects of different complex of soy β‐conglycinin (7S) and isoflavones (7S‐ISO) on osteoporosis in ovariectomized rats. All treatments were administrated intragastrically to the groups every afternoon for 3 months. The treatments were administrated at 1 mL·(100 g)−1, the animals were given 50 mg·kg−1·d−1 ISO, and the concentration of protein was about 2 wt. %. The bone mineral density (BMD) and the bone biomechanics results of left tibia' maximum load in the 7S‐ISO group is significantly higher than in the ovariectomized group and the 7S group (p < .05). Otherwise, the serum tartrate‐resistant acid phosphatase (s‐TRACP), serum osteocalcin (s‐BGP), and serum estradiol (s‐E2) levels in 7S‐ISO were all significantly different from the OVX, OVX + casein, and the OVX + 7S group (p < .05). The serum calcium (s‐Ca) level was not significantly different among all the groups. 7S‐ISO may exhibit moderate estrogenic activities and as compared to 7S and ISO in osteoporosis (OP) of ovariectomized rats. Practical applications The effects of soy proteins on the health of females have always been a concern. It has been extensively reported soy 7S globulin (7S) as a type of trimer glycoprotein can depress blood fats. The aim of this study was to investigate the effects of different complex of soy β‐conglycinin and isoflavones (ISO), the main storage proteins and polyphenols in soy, on osteoporosis in ovariectomized rats.

Onion (Allium cepa L.) Flavonoid Extract Ameliorates Osteoporosis in Rats Facilitating Osteoblast Proliferation and Differentiation in MG-63 Cells and Inhibiting RANKL-Induced Osteoclastogenesis in RAW 264.7 Cells

“…Blood sample was collected from the retro-orbital vein and centrifuged at 1000× g for 15 min at 4 • C to separate serum, and all serum samples were kept at −80 • C. The femurs were isolated as quickly as possible, and tissue was rinsed with phosphate buffer solution (PBS) and fixed in 4% neutral formaldehyde (pH 7.0) for further experiments. Based on previous studies, 50 mg/kg OFE was selected as the medium dose [45][46][47][48], with a half-dose of the medium dose (25 mg/kg) and a 1.5-fold increase in the high dose (75 mg/kg) [49]. And, based on the human dose of alendronate of 10 mg/day, a dose of 1 mg/kg/day was selected for rats.…”

Comparison of three different lactic acid bacteria-fermented proteins on RAW 264.7 osteoclast and MC3T3-E1 osteoblast differentiation

“…SPI, which act as transporters of bioactive ingredients, are well recognized worldwide for its nutritional and functional value 14 . Several studies have suggested that soy protein consumption can lower blood lipid levels, decrease type 2 diabetes occurrence, and increase bone density 15 . Moreover, soy proteins inhibit density reduction in the femur bones of an osteoporotic rat model 16 .…”

“…Moreover, soy proteins inhibit density reduction in the femur bones of an osteoporotic rat model 16 . SPI is composed almost exclusively of two major proteins, namely β-conglycinin and glycinin 17 , which have been shown to have anti-osteoporotic effects in an ovariectomized animal model 15 . Emerging research has demonstrated better immune-boosting effects of fermented soy protein isolates (F-SPI) by functional bacteria than those of SPI alone 18 .…”