Protective effect of Jasonia montana against ethinylestradiol-induced cholestasis in rats

Abstract

Estrogens, and particularly glucuronides such as ethinylestradiol (EE), have been shown to cause cholestasis in animal studies, by reducing bile acid uptake by hepatocytes. The aim of the present article is to investigate anticholestatic activity of the ethanolic extract of the aerial parts of Jasonia montana against liver cholestasis induced by EE in adult female rats in an attempt to understand its mechanism of action, which may pave the way for possible therapeutic applications. Subcutaneous administration of 100 μg/kg b.w. ethinylestradiol to rats induced hepatocellular cholestasis with a significant decrease in serum cholesterol, bile acids and bilirubin levels as well as in hepatic superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) activities and hepatic total, protein-bound and non-protein sulfhydryl groups. Also, treatment with EE produced significant increase in serum Pi-glutathione-s-transferase (Pi-GST), gamma glutamyl transpeptidase (γ-GT) and alpha-glutathione-s-transferase (α-GST) activities as well as serum nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) level and hepatic malondialdehyde (MDA) level as compare to control group. Oral administration of the aerial parts of ethanolic extract at a concentration of 150 mg/kg b.w. daily to rats treated with EE for 15 days showed a significant protection against-induced decrease in serum cholesterol, bile acids and bilirubin levels. The treatment also resulted in a significant increase in hepatic SOD, GPx and GR activities as well as hepatic total, protein-bound and non-protein sulfhydryl groups. In addition, the extract could inhibit serum Pi-GST, γ-GT and α-GST activities as well as reduce serum TNF-α, NO and hepatic MDA as compare to ethinylestradiol treated rats. High content of flavonoids and phenolic compounds was found in ethanolic extract, which may be responsible for free radical activity. The results clearly suggest that the aerial parts of J. montana extract may effectively normalize the impaired antioxidant status in ethinylestradiol (EE)-cholestatic model. Thus the extract may have a therapeutic value in drug-induced biliary cholestasis as well as in hormonal therapy.

POTENTIAL ROLE OF Jasonia montanta AND Jasonia candicans AGAINST ALZHEIMER'S DISEASE: ASSESSMENT OF OXIDATIVE STRESS AND GENE EXPRESSION CHANGES IN AD-INDUCED RATS

“…Hussein and Abdel-Gawad (2010) study greatly supported our results in this concern as they stated that BNO level is significantly depleted after J. montana treatment in rats-induced cholestasis. The high content of flavonoids and phenolic compounds in J. montana extract (Hussein and Abdel-Gawad, 2010) and J. Candicans (Hammerschmidt et al, 1993) may contribut in this effect.…”

“…Moreover, Jasonia species could increase the activities of glutathione peroxidase (Gpx) and glutathione-s-transferase (GST) in the various tissues of rats (Hussein, 2008). Furthermore, Hussein and Abdel-Gawad, (2010) reported that Jasonia species extract effectively normalize the impaired antioxidant status in rats. The phenolic constituents sharing flavonoids in enhancing the total antioxidant capacity in Alzheimer's disease-treated rats.…”

POSSIBLE THERAPEUTIC ROLE OF <I>JASONIA CANDICANS</I> AND <I>JASONIA MONTANA</I> EXTRACTS IN THE REGRESSION OF ALZHEIMER’S DISEASE IN EXPERIMENTAL MODEL

“…These compounds showed an inhibitory effect on serum TNF-α level in iron overload treated rats (Hussein and Farghaly, 2010). Also, Hussein and Abdel-Gawad (2010) reported that the flavonoids (or bioflavonoids) have the ability to suppress many types of transcription factors including TNF-α and H 2 O 2 as these natural compounds possess anti-inflammatory and antioxidant activities (Carlo et al, 1991;Rathee et al, 2006).…”

Anti-inflammatory effect of natural heterocycle glucoside vicine obtained from Vicia faba L. its aglucone (divicine) their effect on some oxidative stress biomarkers in Albino rats

“…[16] Diclofenac sodium has also been reported to suppress inflammation in experimental animal models. [17] As an extension of my interested research program in the extraction and therapeutic evaluation of medicinal plants, [18][19][20][21] I report herein, a facile route to calculate the median lethal doses (LD 50 ) and describe the anti-inflammatory effect of vicine and divicine isolated from fava beans (Vicia faba) which may pave the way for possible therapeutic application.…”