Protective effect of kava constituents in an in vitro model of oral mucositis

Yiannis, Callisthenis; Huang, Kexuan; Tran, An Nhien; Zeng, Cathy; Dao, Emily; Baselyous, Oliver; Mithwani, Muaaz Adil; Paolini, Rita; Cirillo, Nicola; Yap, Tami; McCullough, Michael; Celentano, Antonio

doi:10.1007/s00432-020-03253-3

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…this tendency, the culture with the different eluates of ThLC led to a significantly higher CM-H 2 DCFDA-positive hDPSCs than the control group (p<0.001); while none of the dilutions of BD nor 1:2 and 1:4 ThPT exhibited significant differences. CM-H 2 DCFDA is a chloromethyl derivative of H 2 DCFDA which is used as an indicator for reactive oxygen species (ROS) in cells(33). Upon bacterial infection, hypoxia, or low nutrient supply, dental stem cells produce excessive levels of intracellular ROS which result in oxidative stress.…”

mentioning

confidence: 99%

Comparative Biological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp Stem Cells

Sanz

Soler-Doria

López‐García

et al. 2021

Journal of Endodontics

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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confidence: 99%

Comparative Biological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp Stem Cells

Sanz

Soler-Doria

López‐García

et al. 2021

Journal of Endodontics

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“…dium, commonly containing 0.09 mM calcium supplemented with 30 µg/ml pituitary bovine extract, 0.2 ng/ml EGF, 10% FBS, and ampicillin/streptomycin, has been the primary medium for culturing OKF6/TERT-2 cells in current literature. [5,7,[14][15][16]. Dickson et al [1] described a protocol in which this cell line was cultured in KSFM, which has since been widely cited and replicated [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

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Background: Understanding in vitro expansion of OKF6/TERT-2 oral epithelial cells is important for studying molecular biology of disease and pathology affecting the oral cavity. The media used for any cell culture is paramount in terms of efficient output. Therefore, this study aims to compare two different media for OKF6/TERT-2 cultures: Keratinocyte Serum-Free Medium (KSFM) and a composite medium comprised of DMEM/F-12 mixed with KSFM (referred to as DFK). For application purposes, this investigation also compares the toxicological effects of flavored electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cultures grown in both media. Methods: Cells were grown in KSFM and DFK media and cellular growth, morphology, gene expression of mucins and tight junctions, as well as wound-healing were determined. Additionally, cellular viability and cytotoxicity were indexed after E-liquid exposures. Results: While overall cellular morphologies remained unaltered, cells grown in DFK reached confluency faster. Except for claudin-1, there is no appreciable difference in expression of the other genes tested. Additionally, cultures in DFK appear more sensitive to E-liquids ± flavors. Conclusions: DFK is an alternative medium for cultivation of OKF6/TERT-2 cells to study molecular biology of disease and pathology, such as their responses to E-liquids ± flavors.

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“…MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions, or products referred to in the content. dium, commonly containing 0.09 mM calcium chloride supplemented with 30 µg/mL pituitary bovine extract, 0.2 ng/mL epithelial growth factor (EGF), and 100 U/mL ampicillin/streptomycin, has been the primary medium for culturing OKF6/TERT-2 cells in current literature [5,7,[14][15][16]. Dickson et al [1] described a protocol in which this cell line was cultured in KSFM, which has since been widely cited and replicated [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Cuadra¹,

Shamim²,

Shah³

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Expansion of OKF6/TERT-2 oral epithelial cells in vitro is important for studying the molecular biology of disease and pathology affecting the oral cavity. Keratinocyte Serum-Free Medium (KSFM) is the medium of choice for this cell line. This study compares three media for OKF6/TERT-2 cultures: KSFM, Dulbecco’s Modified Eagle Medium/Nutrient Mixture of Hams F-12 (DMEM/F12) and a composite medium comprised of DMEM/F-12 and KSFM (1:1 v/v), referred as DFK. The toxicological effects of electronic cigarette liquids (E-liquids) on OKF6/TERT-2 cells cultured in these media were also compared. Methods: Cells were cultured in KSFM, DMEM/F12 or DFK and cellular morphology, growth, wound healing and gene expression of mucins and tight junctions were evaluated. Additionally, cytotoxicity was determined after E-liquid exposures. Results: Switching from KSFM to DMEM/F12 or DFK 24-hours post-seeding leads to typical cellular morphologies, and these cultures reach confluency faster than those in KSFM. Wound-healing recovery occurred fastest in DFK. Except for claudin-1, there is no difference in expression of the other genes tested. Additionally, E-liquid cytotoxicity appears to be amplified in DFK cultures. Conclusions: DMEM/F12 and DFK are alternative media for OKF6/TERT-2 cell culture to study molecular biology of disease and pathology, provided cells are initially seeded in KSFM.

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Protective effect of kava constituents in an in vitro model of oral mucositis

Cited by 10 publications

References 30 publications

Comparative Biological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp Stem Cells

Comparative Biological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp Stem Cells

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Comparison of Culture Media for <em>in-vitro</em> Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

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