Protective effect of L‐carnitine on apoptosis, DNA fragmentation, membrane integrity and Lipid peroxidation of spermatozoa in the asthenoteratospermic men

Noreini, Samira Naderi; Malmir, Mahdi; Ghafarizadeh, Aliasghar; Faraji, Tayebeh; Bayat, Razieh

doi:10.1111/and.13932

Cited by 9 publications

(5 citation statements)

References 32 publications

(72 reference statements)

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“…We observed an incremental trend in sperm chromatin decondensation over 24 h of incubation which was consistent with previous studies (Nabi et al, 2014; Naderi Noreini et al, 2021). Indeed, the main product of oxidative stress, 8‐hydroxy, 2'deoxyguanosine, is assumed to be responsible for most of the damage to the sperm DNA (John Aitken & Baker, 2020).…”

Section: Discussionsupporting

confidence: 93%

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Moradi

Hashemian

et al. 2022

Andrologia

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L-Proline is a natural anti-oxidative and osmoprotectant agent, playing a versatile role in cell metabolism and physiology. The present study aimed to explore the antioxidant effects of L-Proline on human sperm function during incubation. Thirty healthy, normozoospermic men (27-40 years) were enrolled. Sperm samples were incubated in an unsupplemented sperm medium (control group), or supplemented with L-Proline (1, 2 and 4 mmol/L) to evaluate its effect during 0, 1, 4 and 24 h of incubation. Sperm were assessed in terms of motility, viability, morphology, chromatin and DNA integrity. Moreover, the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined in the sperm medium. The results indicated that 2 mmol/L of L-Proline significantly improved the maintenance of sperm motility, viability, normal morphology, chromatin and DNA integrity, and TAC levels compared to the control group during 24 h of incubation (p < 0.05). However, 1 and 4 mmol/L of L-Proline could not significantly preserve sperm parameters, chromatin quality, and antioxidant status during different incubation times compared to the control group (p > 0.05). Collectively, the inclusion of L-Proline (2 mmol/L) in the human sperm medium maintains sperm parameters and chromatin quality probably by modulating the oxidative status.

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Section: Discussionsupporting

confidence: 93%

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Moradi

Hashemian

et al. 2022

Andrologia

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“…This function is of great importance since epididymal sperm employ fatty acid oxidation for their energy metabolism; on the contrary, the ejaculated sperm employ the glycolytic process. Besides, carnitines also possess both antioxidant as well as anti-apoptotic properties ( 43 , 44 ) that could counteract the accentuated burden of the seminal OS as well as apoptotic markers in infertile men associated with varicocele ( 8 , 45 , 46 ).…”

Section: Discussionmentioning

confidence: 99%

Seminal L-Carnitine In Infertile Oligoasthenoteratozoospermic Men with Varicocele

Mostafa¹,

Abougabal²,

Mintziori³

et al. 2022

JRI

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Background: Few studies have investigated the relationship of seminal L-Carnitine (LC) with male infertility associated with varicocele. The purpose of this prospective cross-sectional study was to assess seminal plasma LC levels in infertile oligoathenoteratozoospermic (OAT) men with varicocele. Methods: Overall, 86 men were investigated. They were divided into infertile OAT men with varicocele (n=45), infertile OAT men without varicocele (n=21), and fertile men (n=20) as a control group. According to WHO guidelines, these men were subjected to history taking, clinical examination, and semen analysis. Seminal LC levels were evaluated by the colorimetric method. Statistical comparisons were done using Kruskal-Wallis and Mann-Whitney U tests and correlations were verified by the Pearson test. P-value<0.05 was set to be statistically significant. Results: The mean seminal plasma LC levels were significantly lower in infertile OAT men with varicocele (216.3±57.1 ng/ml) compared to infertile OAT men without varicocele (252.9±62.9 ng/ml, p=0.01), or fertile men (382.8±63.6 ng/ml, p= 0.001). Besides, the mean seminal plasma LC level exhibited statistically significant decreases in infertile OAT men of varicocele grade III compared to varicocele grade II cases, and in infertile OAT men with bilateral varicocele compared with unilateral varicocele cases. Collectively, there was a statistically significant positive correlation between seminal LC levels with sperm concentration, motility, and normal morphology. Conclusion: Seminal LC levels are expressively reduced in infertile OAT men with varicocele and are influenced by an increase in varicocele grade and laterality.

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“…As semen was diluted with the treatment, the final concentrations for each pharmaceutical treatment were: BGP-15 10 µM, MitoQ 500 nM and L-Carnitine 3.7 mM. L-Carnitine concentrations were determined by previous literature [ 37 , 38 ], whilst BGP-15 and MitoQ concentrations were chosen because they improved sperm motility and vitality whilst reducing DNA damage in sperm from humans and mice, respectively (unpublished observations). Sterile water (Pfizer Inc., New York, NY, USA) was used as the vehicle to dissolve BGP-15 and L-Carnitine.…”

Section: Methodsmentioning

confidence: 99%

Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds

et al. 2022

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While critical for male fertility preservation, cryopreservation damage reduces sperm quality and fertilization potential. This study investigated whether the addition of mitochondrial-targeted, antioxidant compounds, also known as Mitochondrial activators, to the cryopreservation medium could protect sperm quality during cryopreservation. For this, semen samples from men undergoing IVF/ICSI treatment, which were donated for research, underwent cryopreservation in the absence or presence of BGP-15, MitoQ and L-carnitine. Fresh semen and thawed sperm samples from the same participant were analyzed for indicators of sperm quality: sperm viability, kinetics, mitochondrial reactive oxygen species (ROS) levels, Mitochondrial Membrane Potential (MMP) and DNA damage. Cryopreservation significantly reduced sperm viability and motility and predicted mucous penetration. BGP-15, MitoQ and L-carnitine improved sperm motility, whilst the addition of L-Carnitine prevented the loss of sperm viability during cryopreservation. Both BGP-15 and L-carnitine reduced sperm DNA oxidative damage, but only BGP-15 significantly reduced DNA fragmentation. More importantly, BGP-15 increased sperm predictive mucous penetration and MMP and reduced DNA oxidation. Our results show that the addition of BGP-15 or L-carnitine to the cryopreservation medium improves sperm quality post-thawing, highlighting the potential of mitochondrial antioxidants to improve long-term fertility preservation in males.

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Protective effect of L‐carnitine on apoptosis, DNA fragmentation, membrane integrity and Lipid peroxidation of spermatozoa in the asthenoteratospermic men

Cited by 9 publications

References 32 publications

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Beneficial effect of L‐ Proline supplementation on the quality of human spermatozoa

Seminal L-Carnitine In Infertile Oligoasthenoteratozoospermic Men with Varicocele

Restoring Sperm Quality Post-Cryopreservation Using Mitochondrial-Targeted Compounds

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