Protective effect of latex of Calotropis procera in Freund's Complete Adjuvant induced monoarthritis

Abstract

The protective effect of latex of Calotropis procera in Freund's Complete Adjuvant (FCA) induced monoarticular arthritis was evaluated in rats. Arthritis was induced by a single intra-articular injection of 0.1 mL of 0.1% FCA in the right ankle joint. The effect of dried latex (DL, 200 and 400 mg/ kg) and its methanol extract (MeDL, 50 and 500 mg/ kg) following oral administration was evaluated on joint inflammation, hyperalgesia, locomotor function and histology at the time of peak inflammation. The effects of DL and MeDL were compared with antiinflammatory drugs phenylbutazone (100 mg/ kg), prednisolone (20 mg/kg), rofecoxib (20 and 100 mg/ kg) and immuno-suppressant methotrexate (0.3 mg/ kg). Daily oral administration of DL and its methanol extract (MeDL) produced a significant reduction in joint inflammation (about 50% and 80% inhibition) and associated hyperalgesia. The antihyperalgesic effect of MeDL was comparable to that of rofecoxib. Both DL and MeDL produced a marked improvement in the motility and stair climbing ability of the rats. The histological analysis of the arthritic joint also revealed significant reduction in oedema and cellular infiltration by MeDL that was comparable to that of rofecoxib. Thus, our study suggests that the latex of C. procera has the potential to be used as an antiarthritic agent.

Proteins from latex of Calotropis procera prevent septic shock due to lethal infection by Salmonella enterica serovar Typhimurium

“…However, there is a prevalence of use on inflammatory-related problems. In this case, the ethnopharmacological use of Calotropis procera does not deserve confirmation, since Kumar and co-workers have largely confirmed this folk usage by different experimental approaches and inflammatory models investigated (Kumar and Roy, 2009;Bharti et al, 2010). It is therefore important to advance in understanding mechanisms underlying anti-inflammation; identifying molecules involved and establishing safe use of latex.…”

Ethnomedicinal Plants of the Bawm Tribal Community of Rowangchhari in Bandarban District of Bangladesh

“…The anti-inflammatory effect of stem barks of the first plant has been reported, along with protective effect of latex of the plant against Freund's Complete Adjuvant induced monoarthritis. 15,16 As such, it would be of interest to scientifically study the leaves of this plant as to their effectiveness in relieving pain arising from rheumatism. S. macrophylla is used in traditional medicines of India for treatment of rheumatism.…”

Plant latex: A natural source of pharmaceuticals and pesticides

“…Similarly, methanolic extract of Calotropis procera Latex provides protection against inflammation and oxidative stress in Freund's complete adjuvant-induced monoarthritis in rats. [127] Similarly, Tocotrienols, the unsaturated forms of vitamin E found in tobacco leaves function as efficient antioxidants and protect membrane lipids from peroxidation. [128] Synadenium grantii shows antioxidant/oxidative properties and inhibit production of reactive oxygen species (ROS) and show strong ferric ion reducing power (FRAP), and lipid peroxidation thiobarbituric acid reactive substance (TBARS) activity.…”

“…[131] Similarly, Calotropis procera (family: Apocynaceae) possesses potent anti-inflammatory and analgesic properties. [127] The protein fraction of latex of Calotropis procera shows relieve from inflammation and pain felt by arthritic patient. [127] It shows similar properties when used in animal models.…”

“…[127] The protein fraction of latex of Calotropis procera shows relieve from inflammation and pain felt by arthritic patient. [127] It shows similar properties when used in animal models. [132] Similarly, Himatanthus drasticus (Apocynaceae) latex contain lupeon a pentacyclic triperpene that show anti-inflammatory properties induce synthesis of few bioactive constituents such as pentacyclic triterpene lupeol and lupeol acetate (LA) shows pro-inflammatory cytokines.…”