Protective effect of linalool, myrcene and eucalyptol against t-butyl hydroperoxide induced genotoxicity in bacteria and cultured human cells

Mitić-Ćulafić, Dragana; Žegura, Bojana; Nikolić, Biljana; Vuković-Gačić, Branka; Knežević-Vukčević, Jelena; Filipič, Metka

doi:10.1016/j.fct.2008.11.015

Cited by 153 publications

(74 citation statements)

References 28 publications

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“…51 The ability of both Myrcene and β-Pinene to scavenge the DPPH free radical has been demonstrated 52 It was also reported that Myrcene protected against the genotoxic effect of oxygen reactive species both in bacterial and human cells. 53 In vivo peroxidation assay for testing the antioxidant effects of Myrcene in rats liver have confirmed the antioxidant potential of this monoterpene. 54 Hence, REO from (R-MR) attained second highest antioxidant activity relative to REO from other rosemary samples due to its highest content of these 2 monoterpene hydrocarbons β-Pinene (6.99 ± 0.05 %) and Myrcene (4.24 ± 0.03 %) among all other REO from Rosemary samples (Figure 3 B-D).…”

Section: Reomentioning

confidence: 99%

Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

Koleilat¹,

Raafat²,

El-Lakany³

et al. 2017

Phcog J

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Introduction:The world health organization recommends validating medicinal plants to ensure safety and efficacy. Lavandula is valued for the production of essential oil used in aromatherapy. Rosmarinus is known for its folk use as anti-oxidant and antidiabetic. This study aims to design monographs for Rosmarinus officinalis (Linn.) and Lavandula angustifolia (Linn.) cultivated, in conditions similar to their natural habitat, at Beirut Arab University botanical garden. Also screening the antioxidant activity of both plants, and assessing the antidiabetic effect of R. officinalis (Linn.). Methods: macroscopic and microscopic examination, hydrodistillation to obtain essential oils (EOs), thin layer chromatography, Gas chromatography-Mass spectrometry, and physiochemical properties of the EOs were determined. The antioxidant effect of the EOs is determined by DPPH assay. The antidiabetic effect of Rosemary EO is investigated using alloxan-induced diabetic mice. Results: the qualities of studied species are in agreement with the standards described in WHO monographs and in the British pharmacopeia. Composition of the EO produced from either Rosemary (REO) or Lavender (LEO) collected at different months showed differences. The EO of both showed significant antioxidant effects in DPPH assay (p<0.05). In vivo investigation of the antidiabetic effect of REO showed a significant antidiabetic effect in diabetic mouse model (p<0.05). Conclusion: both plant species and their EOs fulfilled most of standards specified by the British Pharmacopeia 2013. Rosemary collected in April (Spring) showed the best antioxidant activity. Lavender collected in September (Early Fall) showed the best antioxidant activity. REO revealed significant lowering in blood glucose level at the dose of 110 mg/kg.

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Section: Reomentioning

confidence: 99%

Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

Koleilat¹,

Raafat²,

El-Lakany³

et al. 2017

Phcog J

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“…Protective effect against oxidative DNA damage and mutagenesis was determined for Lin, Myr and Euc, since their antioxidative potential has been confirmed by TBA assay [87]. All tested monoterpenes slightly reduced t-BOOH-induced mutagenesis in strains SY252 and IC185 ( Figure 6).…”

Section: Desmutagenic Potential Of Linalool Myrcene and Eucalyptolmentioning

confidence: 99%

“…Euc also decreased spontaneous mutations in IC202, indicating additional mechanisms of antimutagenesis [88]. Protective capacity of Lin, Myr and Euc against oxidative DNA damage was determined in human hepatoma HepG2 and human B lymphoid NC-NC cell lines by alkaline comet assay [87]. Experiments were performed in two experimental protocols, (i) co-treatment of cells with genotoxic agent (t-BOOH) and monoterpene, which tested the ability of the monoterpenes to directly scavenge ROS and (ii) a 20h pre-treatment with monoterpene followed by co-treatment (pre+co-treatment) which, in addition to direct scavenging activity, allows accumulation of monoterpenes in the cell and induction of enzymatic and non-enzymatic cellular antioxidants and detoxifying (Phase II) enzymes.…”

Section: Desmutagenic Potential Of Linalool Myrcene and Eucalyptolmentioning

confidence: 99%

Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)

Nikolić¹,

Mitić-Ćulafić²,

Vuković-Gačić³

et al. 2012

Mutagenesis

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“…The chemopreventive agents exert their effect on DNA replication and repair processes, to modulate the cellular mutagenic processes (Mitic-Culafic et al 2009). DNA nicking assay is employed to check the ability of leaves extract/ fractions of P. aculeata, against the DNA damage caused by free radicals.…”

Section: Dna Nicking Assaymentioning

confidence: 99%

Evaluation of antimutagenic and protective effects of Parkinsonia aculeata L. leaves against H2O2 induced damage in pBR322 DNA

Sharma

Vig

2016

Physiol Mol Biol Plants

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The in vitro antimutagenic and DNA protecting potential of organic (methanol, hexane, n-butanol) and aqueous extract/fractions of Parkinsonia aculeata L. (Fabaceae) was investigated by employing Ames assay and DNA nicking assay. DNA damage by hydroxyl radicals was effectively inhibited by all the extract/fractions. A marked antimutagenic effect was observed against 4-Nitro-o-phenylenediamine and sodium azide (direct acting mutagens) and 2-Aminofluorene (indirect acting mutagen) in TA98 and TA100 strains of Salmonella typhimurium. In Ames assay, two different modes of experiments i.e. pre-incubation and co-incubation were performed and it was observed that all the extract/fractions showed better results in the pre-incubation as compared to co-incubation mode. Out of all the extract/fractions tested, n-butanol fraction was found to be the most effective in preventing DNA damage and inhibiting mutagenesis. UHPLC analysis of extract/fractions revealed presence of polyphenols such as gallic acid, catechin, chlorogenic acid, caffeic acid, umbelliferone, coumaric acid, rutin, and ellagic acid etc. DNA protecting and antimutagenic activity of this plant could be attributed to presence of these polyphenols. The results of this study indicate the presence of potent antioxidant factors in Parkinsonia aculeata L, which are being explored further for their mechanism of action.

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Protective effect of linalool, myrcene and eucalyptol against t-butyl hydroperoxide induced genotoxicity in bacteria and cultured human cells

Cited by 153 publications

References 28 publications

Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)

Evaluation of antimutagenic and protective effects of Parkinsonia aculeata L. leaves against H2O2 induced damage in pBR322 DNA

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