Protective Effect of Linum usitatissimum Aqueous Extract against Hepatotoxicity Induced by Monosodium Glutamate in Male Rats

Abstract

convulsant, antiulcer, analgesics, hepatoprotective, and anti-oxidants activities in vitro and in vivo. [11][12][13][14] As a powerful antioxidant, flaxseed has a role in protecting the liver and kidneys from strong oxidants, 15 and prevent oxidative stress-induced diseases such as renal failure, liver failure, hyperlipidemia, and diabetes. 16 Monosodium glutamate (MSG) was used in Japan as a flavour enhancer in many types of food, producing a flavour called umami meaning savory. 17,18 It is also used in disguised forms, such as in flavoured natural flavours, yeast extract, hydrogenated protein, and in soy protein. Each of these substances contains a percentage of free glutamate, a harmful component of monosodium glutamate. 19 Although it is widely used as a food flavour and catalyst for taste and appetite, studies have indicated that MSG is toxic to humans and laboratory animals especially at high doses. [20][21][22] Therefore, the present study is aimed at evaluating the hepatic effect of aqueous extract of flaxseed alone and in ameliorating MSG-induced hepatotoxicity in rats. Materials and Methods Plant seeds collection and extractionDry seeds of Linum usitatisimium were obtained from the Kerbala market, Iraq. The seeds were identified and authenticated by assists Prof. Nibal M. Tarrad at the Biology Department, University of Kerbala, Iraq. The seeds were cleaned and dried for 48 hours at room temperature. The dried seeds were milled to a fine powder using a mechanical grinder. A 100-gram portion of the powdered seeds sample was suspended in 500 mL of distilled water for 24 hours at room temperature and filtered. The filtrate was then poured into a stainless plate and the