Protective effect of low dose of melatonin against cholestatic oxidative stress after common bile duct ligation in rats

Eşrefoğlu, Mukaddes; Gül, Mehmet; Emre, Memet Hanifi; Polat, Alaaddin; Selimoğlu, Mukadder Ayşe

doi:10.3748/wjg.v11.i13.1951

Cited by 50 publications

(47 citation statements)

References 37 publications

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“…GSH is a key intracellular antioxidant that is capable of interacting with free radicals to repair membrane lipid peroxides. Our data showed BDL-induced cholestasis in developing rat is characterized by accumulation of ROS in the liver is associated with reduced GSH/GSSG ratios, which is in line with adult BDL-induced cholestatic rat (1,4,25,26,33). A relative decrease of GSH and an increase of GSSG in the liver, although both GSH and GSSG levels decrease in plasma, have been reported in adult BDL rats (33).…”

Section: Discussionsupporting

confidence: 61%

Melatonin Ameliorates Bile Duct Ligation-Induced Systemic Oxidative Stress and Spatial Memory Deficits in Developing Rats

et al. 2009

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Bile duct ligation (BDL) induces primary biliary cirrhosis characterized by cholestasis, impaired liver function, and cognition. Young male Sprague-Dawley rats were used: rats underwent laparotomy without BDL ͓sham-control (SC) group͔; rats had restricted diets supply ͓diet-control (DC) group͔; rats underwent BDL for 2 wk (BDL group); BDL rats with melatonin (500 g/kg/d) intraperitoneally for 2 wk ͓melatonin (500 g/kg/d) (M500) group͔; and BDL rats with melatonin (1000 g/kg/d/intraperitoneally) for 2 wk ͓melatonin (1000 g/kg/d) (M1000) group͔. All the surviving rats were assessed for spatial memory and blood was tested for biochemical study. Liver, brain cortex, and hippocampus were collected for determination of malondialdehyde (MDA) and glutathione (GSH)/ oxidized glutathione (GSSG) ratios. BDL group rats had significantly higher plasma direct/total bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), MDA values and higher liver MDA values and lower GSH/GSSG ratios when compared with SC group. In addition, BDL group rats had impaired spatial performance. After melatonin treatment, cholestatic rats' plasma MDA levels, liver MDA levels, and liver GSH/GSSG ratios approached to the values of SC group. Only high dose of melatonin improved spatial performance. Results of this study indicate cholestasis in the developing rats increase oxidative stress and cause spatial memory deficits, which are prevented by melatonin treatment.

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Section: Discussionsupporting

confidence: 61%

Melatonin Ameliorates Bile Duct Ligation-Induced Systemic Oxidative Stress and Spatial Memory Deficits in Developing Rats

et al. 2009

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“…Recent evidence has shown that melatonin has protective effects on liver and hepatic injury after extrahepatic bile duct ligation in rats (Shiesh et al, 2000;Esrefoglu et al, 2005;Ohta et al, 2005). In addition to liver and hepatic damage, free radical accumulation associated with bile duct ligation has been implicated in the genesis of gallstone (Eder et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of Melatonin on Gallbladder Neuromuscular Function in Acute Cholecystitis

Gomez‐Pinilla¹,

Camello²,

Pozo³

2007

J Pharmacol Exp Ther

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“…18 Also reported was a protective effect of Mel against cholestatic oxidative stress, induced by common bile duct ligation in Sprague-Dawley rats. 19 It is worth recalling that Mel can also exert other protective effects against pathological processes occurring as a result of AFB1 administration. Meki et al 20 showed that co-administration of AFB1 (by gastric galvage) and Mel (given either orally or in the form of microcapsules) reduced the apoptotic rate, which was increased by AFB1 alone, in liver tissue.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of melatonin and N‐acetylserotonin on aflatoxin B1‐induced lipid peroxidation in rats

Gesing

Karbownik-Lewińska

2007

Cell Biochemistry & Function

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Aflatoxin B1 (AFB1) is a potent hepatotoxic and hepatocarcinogenic mycotoxin. Reactive oxygen species are considered to participate in the main mechanism of aflatoxin toxicity. Melatonin (Mel) is a hormone which has antioxidative activities. N-acetylserotonin (NAc-5HT) is an immediate precursor of Mel. Melatonin is documented to be completely safe in humans and animals. The aim of our study was to examine the potential protective effects of Mel or NAc-5HT against lipid peroxidation (LPO), caused by AFB1 in male Wistar rats. Mel and NAc-5HT were intraperitoneally (i.p.) injected for 3 weeks in late afternoon (16:00-18:00) injections (20 mg kg(-1) BW/daily). AFB1 (50 microg kg(-1) BW/daily) was administered i.p. 6 h prior to indoleamine injections. Concentrations of malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA), as an index of LPO, were measured in liver, brain, lung, testis and kidney homogenates. The level of LPO in tissue homogenates was expressed as the amount of MDA + 4-HDA (nmol) per milligram of protein. AFB1 increased LPO in the liver, lung, brain and testis, but not the kidney. The increase of LPO caused by AFB1 injections was completely prevented by either Mel or NAc-5HT in all the tissues examined. Melatonin can be considered as a protective pharmacological agent in intoxication with AFB1 and the protective effect of NAc-5HT against aflatoxin-induced LPO broadens the knowledge about its antioxidative properties.

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Protective effect of low dose of melatonin against cholestatic oxidative stress after common bile duct ligation in rats

Abstract: AIM:To investigate the role of oxidative injury and the effect of exogenous melatonin administration on liver damage induced by bile duct ligation (BDL), and second, to evaluate the role of nitric oxide (NO), a free oxygen radical, in oxidative injury.

Cited by 50 publications

References 37 publications

Melatonin Ameliorates Bile Duct Ligation-Induced Systemic Oxidative Stress and Spatial Memory Deficits in Developing Rats

Melatonin Ameliorates Bile Duct Ligation-Induced Systemic Oxidative Stress and Spatial Memory Deficits in Developing Rats

Effects of Melatonin on Gallbladder Neuromuscular Function in Acute Cholecystitis

Protective effects of melatonin and N‐acetylserotonin on aflatoxin B1‐induced lipid peroxidation in rats

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