Protective Effect of Luteolin on an Oxidative-Stress Model Induced by Microinjection of Sodium Nitroprusside in Mice

Nazari, Qand Agha; Kume, Toshiaki; Takada‐Takatori, Yuki; Izumi, Yasukatsu; Akaike, Akinori

doi:10.1254/jphs.13019fp

Cited by 38 publications

(26 citation statements)

References 35 publications

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“…We previously reported that Fe 2+ released from SNP induced oxidative stress and that oxidative stress plays a crucial role in brain damage. 10) We also reported that polyphenols such as luteolin and curcumin had inhibitory effects against the in vivo brain oxidative stress model. 11,12) However, it has not been examined whether compounds that activate the Nrf2-ARE pathway have preventive effects in the model.…”

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confidence: 86%

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Protective Effect of Dimethyl Fumarate on an Oxidative Stress Model Induced by Sodium Nitroprusside in Mice

Kume

Suenaga

Izumi

et al. 2016

Biological & Pharmaceutical Bulletin

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Recent reports have shown that dimethyl fumarate (DMF) prevents brain damage induced by intracerebral hemorrhage and this beneficial effect is mediated by the nuclear erythroid 2 p45-related factor-2-antioxidant response element (Nrf2-ARE) pathway. However, the downstream mechanism underlying the activation of the Nrf2-ARE pathway is unclear. Here, we investigated the protective effect of DMF using an in vivo model of oxidative stress induced by sodium nitroprusside (SNP) and rat primary striatal cultures. Oral administration of DMF prevented SNP-induced motor dysfunction. Pre-administration of DMF (60-200 mg/kg) for 24 h dose-dependently protected against brain damage induced by the striatal injection of SNP. Next, we investigated the protective effect and mechanism of DMF against oxidative stress using rat primary striatal cell cultures. Treatment of striatal cells with DMF (10 µM) markedly prevented hydrogen peroxide-induced cytotoxicity. The protective effect of DMF against oxidative stress in vitro was inhibited by zinc protoporphyrin IX, an inhibitor of heme oxygenase-1, but not by buthionine sulfoximine, an inhibitor of glutathione synthesis. These results suggest that the activation of heme oxygenase-1 plays an important role in the protective effect of DMF.

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confidence: 86%

“…10) We utilize this animal model as an in vivo brain oxidative stress model in mice. We previously reported that Fe 2+ released from SNP induced oxidative stress and that oxidative stress plays a crucial role in brain damage.…”

mentioning

confidence: 99%

Protective Effect of Dimethyl Fumarate on an Oxidative Stress Model Induced by Sodium Nitroprusside in Mice

Kume

Suenaga

Izumi

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Luteolin is a bioflavonoid present in many medicinal plants as well as in some commonly consumed fruits and vegetables including green leafy spices such as parsley, sweet peppers and celery [11–13]. Previous studies showed that luteolin possess numerous beneficial medicinal properties such as antioxidant, antiinflammatory, and anti-allergic actions [15–19]. Data from vitro studies also suggest a protective role of luteolin in the vascular vasculature and the beneficial effect of luteolin on inflammatory process and inflammatory associated CVD [15–19].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies showed that luteolin possess numerous beneficial medicinal properties such as antioxidant, antiinflammatory, and anti-allergic actions [15–19]. Data from vitro studies also suggest a protective role of luteolin in the vascular vasculature and the beneficial effect of luteolin on inflammatory process and inflammatory associated CVD [15–19]. In this context, luteolin at higher doses (≥ 25 μM) inhibited oxidized LDL and TNF-α-induced VCAM-1 expression [15–19].…”

Section: Introductionmentioning

confidence: 99%

“…Data from vitro studies also suggest a protective role of luteolin in the vascular vasculature and the beneficial effect of luteolin on inflammatory process and inflammatory associated CVD [15–19]. In this context, luteolin at higher doses (≥ 25 μM) inhibited oxidized LDL and TNF-α-induced VCAM-1 expression [15–19]. Luteolin at pharmacological concentrations shows lowering plasma lipids [20], inhibiting cholesterol biosynthesis [21], and increasing eNOS gene expression [22].…”

Section: Introductionmentioning

confidence: 99%

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Luteolin protects against vascular inflammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing IΚBα/NF-κB signaling pathway

Jia

Nallasamy

Liu

et al. 2015

The Journal of Nutritional Biochemistry

159

101

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Vascular inflammation plays a significant role in the pathogenesis of atherosclerosis. Luteolin, a naturally-occurring flavanoid, present in many medicinal plants as well as in some commonly consumed fruits and vegetables has received wide attention for its potential to improve vascular function in vitro. However, its effect in vivo and the molecular mechanism of luteolin at physiological concentrations remain unclear. Here, we report that luteolin as low as 0.5 μM significantly inhibited TNF-α-induced adhesion of monocytes to human EA.hy 926 endothelial cells, a key event in triggering vascular inflammation. Luteolin potently suppressed TNF-α-induced expression of the chemokine monocyte chemotactic protein-1 (MCP-1) and adhesion molecules ICAM-1 and VCAM-1, key mediators involved in enhancing endothelial cell-monocyte interaction. Furthermore, luteolin inhibited TNF-α-induced NF-κB transcriptional activity, IκBα degradation, expression of IκB kinase ß (IKKß), and subsequent NF-κB p65 nuclear translocation in endothelial cells, suggesting that luteolin can inhibit inflammation by suppressing NF-κB signaling. In an animal study, C57BL/6 mice were fed a diet containing 0% or 0.6% luteolin for three weeks and luteolin supplementation greatly suppressed TNF-α-induced increases in circulating levels of MCP-1/JE, CXCL1/KC, and sICAM-1 in C57BL/6 mice. Consistently, dietary intake of luteolin significantly reduced TNF-α-stimulated adhesion of monocytes to aortic endothelial cells ex vivo. Histology shows that luteolin treatment prevented the eruption of endothelial lining in the intima layer of the aorta and preserved elastin fibers’ delicate organization as shown by Verhoeff-van Gieson staining. Immunohistochemistry studies further show that luteolin treatment also reduced VCAM-1 and monocyte-derived F4/80-positive macrophages in the aorta of TNF-α-treated mice. In conclusion, luteolin protects against TNF-α-induced vascular inflammation, in both in vitro and in vivo models. This anti-inflammatory effect of luteolin may be mediated via inhibition of the NF-κB-mediated pathway.

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Immunomodulatory Effects of Flavonoids: Possible Induction of T CD4+ Regulatory Cells Through Suppression of mTOR Pathway Signaling Activity

Hosseinzade¹,

Sadeghi²,

Biregani³

et al. 2019

Nutrition and Immunity

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The increasing rate of autoimmune disorders and cancer in recent years has been a controversial issue in all aspects of prevention, diagnosis, prognosis and treatment. Among dietary factors, flavonoids have specific immunomodulatory effects that might be of importance to several cancers. Over different types of immune cells, T lymphocytes play a critical role in protecting the immune system as well as in the pathogenesis of specific autoimmune diseases. One of the important mediators of metabolism and immune system is mTOR, especially in T lymphocytes. In the current review, we assessed the effects of flavonoids on the immune system and then their impact on the mTOR pathway. Flavonoids can suppress mTOR activity and are consequently able to induce the T regulatory subset.

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Protective Effect of Luteolin on an Oxidative-Stress Model Induced by Microinjection of Sodium Nitroprusside in Mice

Cited by 38 publications

References 35 publications

Protective Effect of Dimethyl Fumarate on an Oxidative Stress Model Induced by Sodium Nitroprusside in Mice

Protective Effect of Dimethyl Fumarate on an Oxidative Stress Model Induced by Sodium Nitroprusside in Mice

Luteolin protects against vascular inflammation in mice and TNF-alpha-induced monocyte adhesion to endothelial cells via suppressing IΚBα/NF-κB signaling pathway

Immunomodulatory Effects of Flavonoids: Possible Induction of T CD4+ Regulatory Cells Through Suppression of mTOR Pathway Signaling Activity

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