Protective Effect of Magnesium‐L‐Ascorbyl‐2 Phosphate Against Skin Damage Induced by UVB Irradiation

Abstract

The protective effect of magnesium-L-ascorbyl-2-phosphate (MAP) on cutaneous photodamage such as lipid peroxidation and inflammation induced by ultraviolet B (UVB) exposure (290-320 nm, max. 312 nm) was investigated using hairless mice. When MAP was administered intraperitoneally to mice at a dose of 100 mg of ascorbic acid (AS) per kg body weight base immediately before irradiation (15 kj/m2), the expected increases in thiobarbituric acid reactive substance (TBARS) formation in skin and serum sialic acid, indices of lipid peroxidation and inflammatory reaction, respectively, were significantly reduced. However, the expected decrease in the level of cutaneous AS was unchanged. Similar results were observed for animals given 100 mg of AS-Na per kg body weight before UVB irradiation. When MAP was administered intracutaneously immediately before irradiation, the expected UVB-induced increases in TBARS and sialic acid were again significantly prevented. Ascorbic acid-Na had a less protective effect than intracutaneous MAP administration. The cutaneous AS level was significantly higher in the MAP-treated mice than in the controls, and the UVB-induced decrease in tissue AS was prevented by intracutaneous MAP administration. These results suggest that MAP protects against UVB irradiation-induced lipid peroxidation and inflammation in cutaneous tissue, regardless of the drug administration route. We found, in an in vitro experiment, that MAP was converted to AS as it crossed the epidermis, but that AS-Na did not pass through the epidermis. Furthermore, MAP was also converted to AS in serum. These results suggest that the protective effect of MAP on UVB-induced cutaneous damage is due to conversion of MAP to AS.

Facial hyperpigmentation: causes and treatment

“…79 Because of its instability in aqueous solution, esters like magnesium ascorbyl-2-phosphate (MAP) with similar properties have been used. 80 MAP was found to reduce pigmentation significantly in 19 of 34 patients with melasma and senile freckles but only in three of 25 patients with normal skin. 81 Ascorbic acid can be used alone or in combination therapy.…”

Whitening Effect of Cosmetics Containing Magnesium L-Ascorbyl-2-Phosphate(VC-PMG, Vitamin C Derivatives) Assessed by Colorimeter

“…Besides, natural extracts such as mulberry extract(paper mulberry), licorice extract(glabridin), bearberry extract, A.patula and A. viscida and green tea extract are also known as whitening materials 3 . AsA affects collagen synthesis, drug-metabolizing enzyme, production of interferon, antiviral, antibacterial, and antioxidization and photoprotective actions 13 . AsA does not possess intrinsic absortive capacity for UV radiation and are believed to act after UV radiation has penetrated the skin and interacted with resident skin chromophores to generate reactive oxygen radicals species 14 .…”

Bioavailability and Skin Bioefficacy of Vitamin C and E

“…In vivo, using preclinical models, Kobayashi et al (1996) reported that intraperitoneal (100 mglkg body weight) administration of magnesium-L-ascorbyl-2-phosphate to mice induced a significantly decreased lipid peroxidation in skin, measured by TBARS assay (thiobarbituric acid reactive substance). Chandra Jagetia et al (2003) reported that daily administration of 250 mg/kg body weight of vitamin C before exposure to gamma rays resulted in a significant increase in the activities of superoxide dismutase and glutathione peroxidase as well as glutathione content in the irradiated mouse skin.…”