Protective effect of manganese in cadmium-induced hepatic oxidative damage, changes in cadmium distribution and trace elements level in mice

Abstract

Oxidative tissue damage is considered an early sign of cadmium (Cd) toxicity and has been linked with carcinogenesis. Manganese(II)-at low doses, was found to act as a potent antioxidant against oxidative stress in different in vitro systems producing lipid peroxidation conditions. The present study investigates in vivo antioxidant effects of Mn2+ pretreatment in acute Cd intoxication with regard to lipid peroxidation, antioxidant defense system and cadmium distribution in the tissues of mice. Four groups of male mice (n=7–8) were used: Cd group was injected sc a single dose of CdCl2 · 2½ H2O · (7 mg/kg b.w.); Cd+Mn group was treated ip with MnCl2 · 4H2O (20 mg/kg b.w.) 24 hours before Cd intoxication; Mn group received manganese treatment only; Control group received saline only. Twenty-four hours after Cd intoxication an increased lipid peroxidation (p<0.05), depleted GSH level (p<0.01), increased activity of GSH-Px (p<0.05) and inhibited CAT activity (p<0.01) were found in Cd-treated group compared to controls. Manganese(II) pre-treatment either completely prevented (LP, GSH, GSH-Px) or significantly attenuated (CAT) these changes. Manganese(II) treatment alone decreased LP, enhanced hepatic GSH level and had no effect on antioxidant enzymes compared to control group. A significant increase of Cd concentration in the liver and decreased Cd concentration in the kidneys and testes were found in Cd+Mn treated mice compared to Cd-only treated group. The effect of manganese may result from a different metallothionein induction in particular organs. Manganese(II) pretreatment attenuated the interference of cadmium with Ca homeostasis, the alteration in Zn and Cu levels remained mostly unaffected.

Manganese pre-treatment attenuates cadmium induced hepatotoxicity in Swiss albino mice

“…The antioxidant effect of pre-treatment completely prevented the adverse effect of Cd on LPO and the antioxidant defence system in mouse hepatic system intoxicated to Cd. Studies on rat hepatocytes incubated with Cd suggested that Mn ions may protect from Cd-induced LPO and the inhibition of antioxidant enzymes [19]. The present study was undertaken to evaluate the ameliorative effect of Mn pre-treatment on acute Cd-intoxication in a murine model with regard to oxidative stress biomarkers, enzymatic and non-enzymatic antioxidant defense system in the liver tissues of mice.…”

Antioxidant effect of Mn2+ on capacitation and acrosome reaction of fresh and chilled cattle bull semen

“…Eybl and Kotyzova 17 found that at lower doses Mn 2+ act as potent antioxidant in the protection against oxidative stress. In vitro experiments revealed that the ability of Mn 2+ to scavenge oxygen free radicals generated in differently mediated lipid peroxidations.…”

“…16 It is an essential element required in living organisms both as activator and a constituent of several enzymes. 17 Manganese is an important cofactor of mitochondrial superoxide dismutase (SOD); an antioxidant enzyme which scavenges oxygen free radicals. 18 At low doses, it acts as a potent antioxidant in the protection against oxidative stress.…”

“…18 At low doses, it acts as a potent antioxidant in the protection against oxidative stress. 17,19 Supplementation of Mn to the broiler breeder semen improved the semen quality by enhancing sperm quality index (SQI) and sperm viability. 20 Therefore, present study was planned with an objective to determine the antioxidant effect of Mn 2+ (200 mM) on sperm capacitation and acrosome reaction of fresh and chilled cattle bull semen.…”

Redox dynamics of manganese as a mitochondrial life-death switch

“…Ca levels were increased as Mn concentrations increased, implying a positive correlation. Other heavy metals, such as Cd, have toxicities decreased upon co-administration with Mn, perhaps due to effects on Cd absorption [41, 42]. …”