Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo

Kang, Min‐Cheol; Cha, Seon-Heui; Wijesinghe, W.A.J.P.; Kang, Sung-Myung; Lee, Seung‐Hong; Kim, Eun-A.; Song, Choon Bok; Jeon, You‐Jin

doi:10.1016/j.foodchem.2012.11.005

Cited by 122 publications

(81 citation statements)

References 24 publications

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“…It is reported that 8.8'-bieckol, 6.6'-bieckol, 7-phloroeckol, phlorofurofucoeckol A, eckol, and 2-phloroeckoli, which offer the antioxidant, anti-inflammatory, and anti-allergic properties of ECP extracts, carry many different phlorotannin molecules such as phlorotannin A (12)(13)(14). Polyphenols are electron-rich compounds and can inhibit the formation of oxidative radicals by facilitating electron donor reactions (15).…”

Section: Discussionmentioning

confidence: 99%

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2017

Turk Arch Otorhinolaryngol

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Objective: Cisplatin is a widely used agent for the treatment of adult and childhood malignancies. Side effects such as nephrotoxicity, neurotoxicity, and ototoxicity lead to dose limitations. Ecklonia cava polyphenol extract (ECP) is a molecule obtained from algae that live in seawater in the Far East. ECP has recently been shown to have protective effects against oxidative stress. The aim of this study was to evaluate the possible protective effects of ECP on cisplatin ototoxicity. Methods:In this study, we investigated the protective effects of ECP against cisplatin-induced cell death in mouse-derived House Ear Institute Organ of Corti (HEI-OC1) cochlear cells. Cisplatin (100 µM) and 1, 10, and 25 µM doses of ECP were administered to the cells, and the protective effects of ECP at 24 and 72 hours were investigated. Cell viability was evaluated by the WST-1 (water soluble tetrazolium salt).Results: Cisplatin (100 µM) reduced cell viability in both the 24 th and 72 nd hour evaluation. Although the 25 µM dose of ECP showed otoprotective effects in the 24 th hour, in the 72 nd hour this effect disappeared. Other doses of ECP showed no otoprotective effects in the 24 th and 72 nd hours. Conclusion:Although ECP showed some protective effects in the 24 th hour against cisplatin ototoxicity, these effects disappeared by the 72 nd hour. Further studies using recurrent and higher doses of ECP are required.

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Section: Discussionmentioning

confidence: 99%

Untitled

2017

Turk Arch Otorhinolaryngol

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“…The heart-beating rate was measured over 3 min using a microscope (Olympus, Japan), and results are represented as the average heart-beating rate per min . Cell death was detected in live embryos using acridine orange staining at 72 hpf (Kang et al 2013). For teratotoxicity assay, after zebrafish embryos exposed to SCDE for 24 and 48 hpf, non-lethal malformations were observed under the microscope (Leica, Leica Microsystems, Bannockburn, IL).…”

Section: Measurement Of Embryo Toxicitymentioning

confidence: 99%

“…Thereafter, zebrafish embryos were transferred into fresh embryo medium, where they developed for up to 72 hpf. Cell death and intracellular ROS and NO generation in zebrafish embryos were estimated according to previously reported methods (Kang et al 2013;Lee et al 2013).…”

Section: Evaluation Of Cell Death and Generation Of Intracellular Reamentioning

confidence: 99%

Developmental toxicity and anti-inflammatory effect of the soft coral Dendronephthya gigantea collected from Jeju Island in zebrafish model

Lee

2017

Fish Aquatic Sci

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Recent in vitro studies have demonstrated that extract of soft coral Dendronephthya gigantea (SCDE) had strong anti-inflammatory activities. However, the direct effects of SCDE on anti-inflammatory activities in vivo model remained to be determined. Therefore, the present study was designed to assess in vivo anti-inflammatory effect of SCDE using lipopolysaccharide (LPS)-stimulated zebrafish model. We also investigated whether SCDE has toxic effects in zebrafish model. The survival, heart beat rate, and developmental abnormalities were no significant change in the zebrafish embryos exposed to at a concentration below 100 μg/ml of SCDE. However, lethal toxicity was caused after exposure to 200 and 400 μg/ml of SCDE. Treating zebrafish model with LPS treatment significantly increased the reactive oxygen species (ROS) and nitric oxide (NO) generation. However, SCDE inhibited this LPS-stimulated ROS and NO generation in a dose-dependent manner. These results show that SCDE alleviated inflammation by inhibiting the ROS and NO generation induced by LPS treatment. In addition, SCDE has a protective effect against the cell damage induced by LPS exposure in zebrafish embryos. This outcome could explain the profound anti-inflammatory effect of SCDE both in vitro as well as in vivo, suggesting that the SCDE might be a strong anti-inflammatory agent.

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“…Formation of ROS in medaka embryos was measured spectrophotometrically using an oxidation-sensitive fluorescent probe, 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) as previously described. 29 Briefly, the embryos (∼4 hpf) were treated with various forms of copper, that is, CuO-NPs, CuSO 4 and sulfidized CuO-NPs, respectively. After 48-h exposure, the embryos were transferred into 96-well plates and treated with DCFH-DA solution (50 μM), and the plates were incubated for 1 h in the dark at room temperature.…”

Section: −28mentioning

confidence: 99%

Sulfidation as a Natural Antidote to Metallic Nanoparticles Is Overestimated: CuO Sulfidation Yields CuS Nanoparticles with Increased Toxicity in Medaka (Oryzias latipes) Embryos

Zhou

et al. 2015

Environ. Sci. Technol.

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Sulfidation is considered as a natural antidote to toxicity of metallic nanoparticles (NPs). The detoxification contribution from sulfidation, however, may vary depending on sulfidation mechanisms. Here we present the dissolution−precipitation instead of direct solid-stateshell mechanism to illustrate the process of CuO-NPs conversion to CuSNPs in aqueous solutions. Accordingly, the CuS-NPs at environmentally relevant concentrations showed much stronger interference on Japanese medaka (Oryzias latipes) embryo hatching than CuO-NPs, which was probably due to elevated free copper ions released from CuS-NPs, leading to significant increase in oxidative stress and causing toxicity in embryos. The larval length was significantly reduced by CuS-NPs, however, no other obviously abnormal morphological features were identified in the hatched larvae. Co-introduction of a metal ion chelator [ethylene diamine tetraacetic acid (EDTA)] could abolish the hatching inhibition induced by CuS-NPs, indicating free copper ions released from CuS-NPs play an important role in hatching interference. This work documents for the first time that sulfidation as a natural antidote to metallic NPs is being overestimated, which has far reaching implications for risk assessment of metallic NPs in aquatic environment.

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Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo

Cited by 122 publications

References 24 publications

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Developmental toxicity and anti-inflammatory effect of the soft coral Dendronephthya gigantea collected from Jeju Island in zebrafish model

Sulfidation as a Natural Antidote to Metallic Nanoparticles Is Overestimated: CuO Sulfidation Yields CuS Nanoparticles with Increased Toxicity in Medaka (Oryzias latipes) Embryos

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