Protective effect of medicinal plant extracts on biomarkers of oxidative stress in erythrocytes

Luqman, Suaib; Kaushik, Shubhangi; Srivastava, Sangeeta; Kumar, Ritesh; Bawankule, Dnyaneshwar Umrao; Pal, Anirban; Darokar, Mahendra P.; Khanuja, S. P. S.

doi:10.1080/13880200902832900

Cited by 23 publications

(16 citation statements)

References 51 publications

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“…Ethanol extracts increased the GSH level more than ethanol–water ones (S6–S8), in agreement with previous studies 45 where ethanol extracts of 6 studied plants provided better protection of erythrocyte GSH levels than corresponding aqueous extracts. At a concentration of 50 µg/ml, the strongest effect on the GSH level was observed for the ethanol extract from flowers collected on 26.06.…”

Section: Resultssupporting

confidence: 92%

Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers

Makarova

Sajkowska-Kozielewicz

Zawada

et al. 2021

Sci Rep

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The polyphenol content and antioxidant capacity of hyperforin and hypericin-standardized H. perforatum L. extracts may vary due to the harvest time. In this work, ethanol and ethanol–water extracts of air-dried and lyophilized flowers of H. perforatum L., collected throughout a vegetation season in central Poland, were studied. Air-dried flowers extracts had higher polyphenol (371 mg GAE/g) and flavonoid (160 mg CAE/g) content, DPPH radical scavenging (1672 mg DPPH/g), ORAC (5214 µmol TE/g) and FRAP (2.54 mmol Fe2+/g) than lyophilized flowers extracts (238 mg GAE/g, 107 mg CAE/g, 1287 mg DPPH/g, 3313 µmol TE/g and 0.31 mmol Fe2+/g, respectively). Principal component analysis showed that the collection date influenced the flavonoid and polyphenol contents and FRAP of ethanol extracts, and DPPH and ORAC values of ethanol–water extracts. The ethanol extracts with the highest polyphenol and flavonoid content protected human erythrocytes against bisphenol A-induced damage. Both high field and benchtop NMR spectra of selected extracts, revealed differences in composition caused by extraction solvent and raw material collection date. Moreover, we have shown that benchtop NMR can be used to detect the compositional variation of extracts if the assignment of signals is done previously.

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Section: Resultssupporting

confidence: 92%

Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers

Makarova

Sajkowska-Kozielewicz

Zawada

et al. 2021

Sci Rep

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“…However, the hydroxyl groups at C-3 and C-5 do not seem to be important for this inhibition, as the C-3 hydroxyl group in rutin (which inhibited lipid peroxidation) is replaced by a disaccharide and the flavonoids which have C-5 hydroxyl groups did not inhibit lipid peroxidation. These results are consistent with those of other researchers [32] who found that quercetin protected H 2 O 2 induced lipid peroxidation of erythrocytes, and with others [33] who found that quercetin and rutin protected low-density lipoproteins (LDL) against Cu 2+ ion-dependent oxidation, and also with others [34] who found that flavonoids which possess only one hydroxyl group at C-4' on the B ring and which also lack the C-3 hydroxyl group (i.e. apigenin and chrysin) did not show a scavenging activity towards peroxynitrite, whereas, quercetin showed the most prominent scavenging activity of all the tested flavonoids.…”

Section: Discussionsupporting

confidence: 94%

Structure-activity relationships regarding the antioxidant effects of the flavonoids on human erythrocytes

Bilto

Suboh²,

Aburjai³

et al. 2012

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The effects of eleven flavonoids on lipid peroxidation, protein degradation, deformability and osmotic fragility of human erythrocytes exposed in vitro to 10 mM H2O2 for 60 min at 37 ºC have been studied. The following flavonoids; quercetin, rutin and morin significantly protected eryt-hrocytes against lipid peroxidation caused by H2O2. This inhibition of lipid peroxidation could be explained by the presence of at least two hydroxyl groups in ring B of the flavonoid structure, regardless of their positions. However, the flavonoids; quercetin, 3,5,7-trihy- droxy-4'-methoxy flavone-7-rutinoside and 3- hydroxy flavone significantly protected eryt-hrocytes against protein degradation. This inhibition could also be explained by the presence of a hydroxyl group at C-3 in ring C of the flavonoid structure. Quercetin and 3,5,7-trihydroxy-4'- methoxy flvone-7-rutinoside significantly protected erythrocytes against loss of deformability and increased osmotic fragility, indicating that the loss of erythrocyte deformability and the increase in osmotic fragility of erythrocytes exposed to H2O2 are related to protein degradation rather than to lipid peroxidation. The other flavonoids (chrysin, 2-carboxy ethyl dihydroxy flavone, apigenin, cirsimaritin, α-naphto flavone and flavanone) failed to protect erythrocytes against the observed oxidative damages. The results demonstrate the importance of the chemical groups substituted on the basic skeleton of the flavonoids in dictating the type of antioxidant activity, and also demonstrate the hemorheological potentials of flavonoids that have particular protein-antioxidant activities

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“…En muchos casos, los estudios se han basado en investigaciones in vitro (en células en cultivo en el laboratorio) o bien han empleado animales como objeto de estudio. Estos estudios aportan explicaciones mecanicistas para algunos de los supuestos beneficios que han surgido de la medicina tradicional (Luqman et al, 2009). Por ejemplo, muchos de estos estudios han comprobado que el tratamiento con la moringa o sus extractos eleva los niveles de una variedad de biomarcadores, antioxidantes y enzimas de destoxificación (Fahey et al, 2004;Faizi et al, 1994;Kumar y Pari, 2003;Rao et al, 1999).…”

Section: Introductionunclassified

Moringa oleifera: un árbol multiusos para las zonas tropicales seca

Olson

Fahey

2011

Rev.Mex.Biodiv.

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Resumen. En zonas tropicales secas, el árbol de la moringa (Moringa oleifera Lam.) es objeto de gran atención por parte de los productores, en tanto que crece el número de proveedores que promueven la planta como panacea. Ante esta situación, es necesario separar los usos que están identificados y fundamentados por el conocimiento científico de aquellos que no lo están. Con base en el presente estudio y en la literatura, se presenta un resumen de las bases científicas que sustentan algunos de los beneficios de la moringa, en la medida de lo que hasta hoy se conoce. Los análisis comprueban que la harina de hoja de la moringa se compara favorablemente con la leche en polvo en cuanto a sus componentes de proteína y de calcio y tiene, adicionalmente, un alto contenido de vitamina A. Además de su valor nutritivo, las hojas son ricas en antioxidantes, entre los cuales destacan los isotiocianatos, los cuales parecen presentar propiedades anticancerígenas, hipotensoras, hipoglucemiantes y antibióticas. Las concentraciones de factores antinutritivos en las hojas, tales como inhibidores de proteasas, taninos, saponinas y lectinas, son insignificantes. La mayoría de los estudios sobre las cualidades benéficas de la moringa se han llevado a cabo in vitro o en animales; por lo tanto, se desconocen las dosis necesarias para producir algún efecto benéfico en humanos. Sin embargo, dado que los beneficios potenciales son muchos y los niveles de sustancias antinutricionales bajísimos, no encontramos argumentos en contra del consumo de la planta. En resumen, Moringa oleifera es un alimento nutritivo y benéfico que ofrece características muy atractivas para establecer su cultivo en comunidades sostenibles en el trópico seco de México y otros países de Latinoamérica.

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Protective effect of medicinal plant extracts on biomarkers of oxidative stress in erythrocytes

Cited by 23 publications

References 51 publications

Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers

Harvest time affects antioxidant capacity, total polyphenol and flavonoid content of Polish St John’s wort’s (Hypericum perforatum L.) flowers

Structure-activity relationships regarding the antioxidant effects of the flavonoids on human erythrocytes

Moringa oleifera: un árbol multiusos para las zonas tropicales seca

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