Protective effect of melatonin against multistress condition induced lipid peroxidation via measurement of gastric mucosal lesion and plasma malondialdehyde levels in rats

Abstract

AIM:To study the protective effect of a natural antioxidant, melatonin, against multistress condition induced lipid peroxidation via determination of gastric damage and plasma malondialdehyde (MDA) level by high performance liquid chromatography in rats. METHODS:We compared indomethacin-induced gastric damage and MDA plasma level in three groups of rats: unoperated, bile duct ligated and sham-operated and evaluated the role of the melatonin on gastric damage and plasma MDA level. Indomethacin and melatonin were injected intraperitoneally in doses of 50 mg/kg and 20 mg/kg, respectively. Animals were killed 4 h after indomethacin injection. RESULTS:Indomethacin induced more severe gastric damage and plasma MDA level in bile duct ligated animals was significantly higher (3.1 ± 0.04 µmol/L) than sham (2.8 ± 0.04 µmol/L) and unoperated animals (1.4 ± 0.08 µmol/L). Pretreatment with melatonin reduced indomethacin-induced gastric damage and plasma MDA level. CONCLUSION:Considering the results of this study, we suggest that in multistress conditions the intensity of gastric damage and the plasma MDA level are great and melatonin reduces the negative effect of lipid peroxidation and cell damage by oxidative stress in multistress conditions due to its antioxidizing activity.

α1‐ and α2‐Adrenoceptor hyporesponsiveness in isolated bisected vas deferens of bile duct‐ligated rats

“…In the sham‐operated controls, the bile duct become manipulated and was left in situ . In the BDL rats, the bile duct was become doubly ligated and then the abdomen was closed in two layers (Kiarostami et al. , 2006).…”

The effect of morphine on development of ulcer lesions of the rats exposed to indomethacin induced stress

“…Najnovija eksperimentalna istraživanja potvrđuju da NSAIL pored inhibicije sinteze prostaglandina, koče sintezu i sekreciju mukusa, kao i sekreciju bikarbonata, podstiču sekreciju HCl i pepsina, što sve zajedno izaziva ishemiju. Kao posledica ishemije razvija se oksidativni stres u ćelijama mukoze želuca, odnosno u tkivu se nagomilavaju reaktivni kiseonični radikali (ROS), koji stupaju u reakciju sa proteinima, lipidima i DNA ćelija mukoze želuca [29,30]. Mnogi autori ukazuju na prooksidantno dejstvo indometacina u mukozi želuca pacova, koje se manifestuje povećanjem intenziteta lipidne peroksidacije (povećan sadržaj malondialdehida -MDA), smanjenjem sadržaja redukovanog glutationa i smanjenjem aktivnosti superoksid dizmutaze (SOD), katalaze (CAT) i glutation peroksidaze (GSHPx) [31,32,33,34].…”