Protective effect of melatonin and pinoline on nitric oxide-induced lipid and protein peroxidation in rat brain homogenates

Piñol‐Ripoll, Gerard; Fuentes-Broto, Lorena; Millán-Plano, Sergio; Reyes-Gonzales, Marcos César; Martı́nez-Balları́n, Enrique; Reiter, R. J.; García, Joaquín J.

doi:10.1016/j.neulet.2006.06.031

Cited by 18 publications

(18 citation statements)

References 33 publications

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“…Herein, we show for the first time that tryptoline also reduces oxidative stress in synaptosomal membranes. As for pinoline, our data are in agreement with two previous studies stating that pinoline limits oxidative damage to proteins in synaptosomes [24,60]. …”

Section: Resultssupporting

confidence: 92%

“…The data reported here for β-carbolines is consistent with this observation. Several reports have claimed that pinoline reduced lipid peroxidation induced by hydrogen peroxide, iron, sodium nitroprussiate, copper (I) iodide, aluminum, and glutamate [24,44,60,68–71]. Herein, we show for the first time that tryptoline also reduces oxidative stress in synaptosomal membranes.…”

Section: Resultsmentioning

confidence: 48%

“…Previous in vitro evidence indicates that melatonin protects synaptosomal membranes against lipid peroxidation mediated by aluminum and sodium nitroprussiate [24,60,61]. The reduction of carbonylation of proteins by melatonin was described for the first time by Kim et al [62], who proved that melatonin reduced the formation of carbonyl groups in a solution of bovine serum albumin treated with ascorbate-Fe 3+ -EDTA.…”

Section: Resultsmentioning

confidence: 99%

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Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

Millán-Plano

Piedrafita

Miana-Mena

et al. 2010

IJMS

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Since biological membranes are composed of lipids and proteins we tested the in vitro antioxidant properties of several indoleamines from the tryptophan metabolic pathway in the pineal gland against oxidative damage to lipids and proteins of synaptosomes isolated from the rat brain. Free radicals were generated by incubation with 0.1 mM FeCl3, and 0.1 mM ascorbic acid. Levels of malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA), and carbonyl content in the proteins were measured as indices of oxidative damage to lipids and proteins, respectively. Pinoline was the most powerful antioxidant evaluated, with melatonin, N-acetylserotonin, 5-hydroxytryptophan, 5-methoxytryptamine, 5-methoxytryptophol, and tryptoline also acting as antioxidants.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

Millán-Plano

Piedrafita

Miana-Mena

et al. 2010

IJMS

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“…Brain, as the central nervous system, is poor in antioxidant defense system, because it is protected by the blood-brain barrier defending the brain from the pass of toxins, but also limiting the movement of the antioxidant [56,57]. In the present study, activity of SOD in fish brain of E2 group was significantly higher after 21 d exposure, probably due to a response to toxicant stress and serves to neutralize the impact of increased ROS generation.…”

Section: Antioxidant Indicesmentioning

confidence: 44%

Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): Effects on morphological indices, hematological parameters and antioxidant responses

Velíšek

Žlábek

et al. 2011

Journal of Hazardous Materials

128

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“…For example, the authors showed the protective effect of Mel on nitric oxide-induced lipid and protein peroxidation in rat brain homogenates. 16 Also, Mel inhibited lipid peroxidation (LPO) in the testicular tissue of streptozocin-induced diabetic rats. 17 Other authors demonstrated that Mel reduced the oxidative stress caused by cisplatin in rat testicular tissue.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of melatonin and N‐acetylserotonin on aflatoxin B1‐induced lipid peroxidation in rats

Gesing

Karbownik-Lewińska

2007

Cell Biochemistry & Function

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Aflatoxin B1 (AFB1) is a potent hepatotoxic and hepatocarcinogenic mycotoxin. Reactive oxygen species are considered to participate in the main mechanism of aflatoxin toxicity. Melatonin (Mel) is a hormone which has antioxidative activities. N-acetylserotonin (NAc-5HT) is an immediate precursor of Mel. Melatonin is documented to be completely safe in humans and animals. The aim of our study was to examine the potential protective effects of Mel or NAc-5HT against lipid peroxidation (LPO), caused by AFB1 in male Wistar rats. Mel and NAc-5HT were intraperitoneally (i.p.) injected for 3 weeks in late afternoon (16:00-18:00) injections (20 mg kg(-1) BW/daily). AFB1 (50 microg kg(-1) BW/daily) was administered i.p. 6 h prior to indoleamine injections. Concentrations of malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA), as an index of LPO, were measured in liver, brain, lung, testis and kidney homogenates. The level of LPO in tissue homogenates was expressed as the amount of MDA + 4-HDA (nmol) per milligram of protein. AFB1 increased LPO in the liver, lung, brain and testis, but not the kidney. The increase of LPO caused by AFB1 injections was completely prevented by either Mel or NAc-5HT in all the tissues examined. Melatonin can be considered as a protective pharmacological agent in intoxication with AFB1 and the protective effect of NAc-5HT against aflatoxin-induced LPO broadens the knowledge about its antioxidative properties.

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Protective effect of melatonin and pinoline on nitric oxide-induced lipid and protein peroxidation in rat brain homogenates

Cited by 18 publications

References 33 publications

Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

Melatonin and Structurally-Related Compounds Protect Synaptosomal Membranes from Free Radical Damage

Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): Effects on morphological indices, hematological parameters and antioxidant responses

Protective effects of melatonin and N‐acetylserotonin on aflatoxin B1‐induced lipid peroxidation in rats

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