Protective effect of myricetin on LPS-induced mastitis in mice through ERK1/2 and p38 protein author

Kan, Xingchi; Liu, Juxiong; Chen, Yingsheng; Guo, Wenjin; Xu, Dianwen; Cheng, Ji; Yang, Zhan-Qing; Fu, Shoupeng

doi:10.1007/s00210-021-02069-3

Cited by 10 publications

(7 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typically, mastitis, which severely hinders dairy production, is caused by microbial infections that induce inflammation in the mammary gland [ 26 ]. One of the most common such pathogenic microorganisms is E coli, which is a major cause of severe mastitis [ 27 ]. Although antibiotics have been proven to be effective in treating mastitis, antibiotic residues left behind in milk and dairy products pose a health risk to humans.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, the effect of allicin on the cytosolic inflammatory response in the mammary epithelial cells of cows was analyzed by assessing the TLR4/NF-κB pathway after LPS stimulation. After establishing a model of mouse mastitis, we investigated whether allicin can reduce the expression of inflammatory factors in the mouse mammary gland and thereby alleviate LPS-induced mastitis in mice [ 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells

Che

Zhou

Lyu

et al. 2023

IJMS

View full text Add to dashboard Cite

Dairy farming is the most important economic activity in animal husbandry. Mastitis is the most common disease in dairy cattle and has a significant impact on milk quality and yield. The natural extract allicin, which is the main active ingredient of the sulfur-containing organic compounds in garlic, has anti-inflammatory, anticancer, antioxidant, and antibacterial properties; however, the specific mechanism underlying its effect on mastitis in dairy cows needs to be determined. Therefore, in this study, whether allicin can reduce lipopolysaccharide (LPS)-induced inflammation in the mammary epithelium of dairy cows was investigated. A cellular model of mammary inflammation was established by pretreating bovine mammary epithelial cells (MAC-T) with 10 µg/mL LPS, and the cultures were then treated with varying concentrations of allicin (0, 1, 2.5, 5, and 7.5 µM) added to the culture medium. MAC-T cells were examined using RT–qPCR and Western blotting to determine the effect of allicin. Subsequently, the level of phosphorylated nuclear factor kappa-B (NF-κB) was measured to further explore the mechanism underlying the effect of allicin on bovine mammary epithelial cell inflammation. Treatment with 2.5 µM allicin considerably decreased the LPS-induced increase in the levels of the inflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) and inhibited activation of the NOD-like receptor protein 3 (NLRP3) inflammasome in cow mammary epithelial cells. Further research revealed that allicin also inhibited the phosphorylation of inhibitors of nuclear factor kappa-B-α (IκB-α) and NF-κB p65. In mice, LPS-induced mastitis was also ameliorated by allicin. Therefore, we hypothesize that allicin alleviated LPS-induced inflammation in the mammary epithelial cells of cows probably by affecting the TLR4/NF-κB signaling pathway. Allicin will likely become an alternative to antibiotics for the treatment of mastitis in cows.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells

Che

Zhou

Lyu

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…ERK1/2, an extracellular signal‐regulated kinase, is one of the MAPK pathway (Lake et al, 2016). Many studies have shown that ERK1/2 is significantly expressed during inflammation responses, such as LPS‐induced mouse mastitis (Kan et al, 2021) and increased phosphorylation of ERK1/2 in BMECs (Jeong et al, 2017), thus becoming a target for many inflammation treatments. SPDYA, a member of the cell cycle regulator family, binds to G1/S and G2/M CDKs and controls cell survival through atypical activation of CDKs (Al Sorkhy et al, 2012) and activation of ERK1/2 in an MEK‐independent manner.…”

Section: Discussionmentioning

confidence: 99%

Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid

Shen

Yan

et al. 2022

Animal Physiology Nutrition

View full text Add to dashboard Cite

Mastitis is one of the most common diseases of dairy cattle and can be caused by physical stress, chemicals and microbial infection. Staphylococcus aureus is the most common pathogens that induce mastitis in dairy cattle. In this study, bovine mammary epithelial cells (BMECs) were treated either with lipoteichoic acid (LTA, 30 µg/ml) or 1 × phosphate‐buffer saline (PBS, control) and RNA‐Seq was applied to explore the effect of LTA on the expression microRNAs (miRNAs) in BMECs. Compared to the control group, 43 miRNAs were significantly up‐regulated and eight miRNAs were significantly down‐regulated. Additionally, 724 genes were significantly up‐regulated and 13 genes were significantly down‐regulated in LTA group relative to the control group. Bta‐miR‐196a, bta‐miR‐2285aj‐5p, bta‐miR‐143, bta‐miR‐2433, bta‐miR‐2284f and bta‐miR‐2368‐3p were selected from 51 differentially expressed miRNAs and are discussed in this manuscript. Target gene prediction revealed that the target genes of these six miRNAs were all differentially expressed, including MT1E, SPDYA, FGL1, TLR2, PAPOLG, ZDHHC17 and SMC4. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the target genes with differentially expressed miRNAs were enriched in mitogen‐activated protein kinase (MAPK) signalling pathway, rheumatoid arthritis and cancer. Therefore, the results of this study provided new evidences for the molecular mechanism of LTA‐induced mastitis, which may provide new targets for the diagnosis and treatment of mastitis in dairy cattle.

show abstract

“…Colon cancer ↓ TNF-𝛼, IL-1𝛽, IL-6, NF-𝜅B, p-NF-𝜅B, COX-2, PCNA, and Cyclin D1 [ 104] RAW264.7 cells ↓ GP91 phosphorylation, p47 phosphorylation, JAKs, and STAT1 [ 106] Kaempferol Proliferation Ovarian cancer ↑ DR4, DR5, CHOP, JNK, ERK1/2, p38 [26] Gastric cancer ↓ NF-𝜅B and MAPK signaling pathways [27] Colon cancer ↓ CDK2, CDK4, cyclin A/D/E [28] Ovarian cancer ↑ p21, p-Chk2, p-Cdc25C, p-Cdc2 [29] Apigenin Proliferation Prostate cancer ↓ Rb, p-Rb (Ser807/811), phospho-Rb (Ser780), p-ELK-1, c-FOS, and cyclin D1; ↑ p-ERK1/2, p-JNK1/2 [41] Melanoma ↓ p-ERK1/2, p-AKT, and p-mTOR [43] Breast cancer ↑ cl-caspase-8, cl-caspase-3, and cl-PARP; ↓ p-JAK2 and p-STAT3 [40] Cervical cancer ↑ p16, INK4A; ↓ cyclin A/D/E and CDK2/6; ↓ the PTEN/PI3K/AKT pathway [42] Angiogenesis Esophageal cancer ↓ VEGF and IL-6 expression [91] Melanoma ↓ IL-6, IL-10 and TNF-ɑ [92] Immunity inflammation Colonic inflammation ↓MPO, inflammatory cytokines, and COX-2 [ 108] Pancreatic cancer ↓ SK-3𝛽/NF-𝜅B signaling pathway [10] NSCLC ↓ IFN-𝛾-induced PD-L1 expression [ 112] Hesperetin Proliferation Breast cancer ↓ HER2, MMP-9, Rac1; arrested cell cycle [46] ↑ p53, NOTCH1, PPARG; ↓ 𝛽-catenin [48] Gastric cancer ↓PI3K/Akt signaling pathway; ↑PTEN [50] Immunity inflammation Hepatic cancer ↑ Nrf2, PPAR𝛾, and HO-1 [ 106] Gastric cancer ↑cl-caspase3, cl-PARP, and P53↓ Akt and p-Akt [ 116] Quercetin…”

Section: Migration and Invasionmentioning

confidence: 99%

A Review on Dietary Flavonoids as Modulators of the Tumor Microenvironment

Duan

Zhou

et al. 2023

Molecular Nutrition Food Res

View full text Add to dashboard Cite

The tumor microenvironment (TME) is the local environment where malignant cells strive and survive, composed of cancer cells and their surroundings, regulating essential tumor survival, and promotion functions. Dietary flavonoids are abundantly present in common vegetables and fruits and exhibit good anti‐cancer activities, which significantly inhibit tumorigenesis by targeting TME constituents and their interaction with cancer cells. This review aims to synthesize information concerning the modulation of TME by dietary flavonoids, as well as to provide insights into the molecular basis of its potential anti‐tumor activities, with an emphasis on its ability to control intracellular signaling cascades that regulate the TME processes, involving cell proliferation, invasion and migration, continuous angiogenesis, and immune inflammation. This study will provide a theoretical basis for the development of the leading compound targeting TME for anti‐cancer therapies from these dietary flavonoids.

show abstract

Protective effect of myricetin on LPS-induced mastitis in mice through ERK1/2 and p38 protein author

Cited by 10 publications

References 39 publications

Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells

Allicin Alleviated LPS-Induced Mastitis via the TLR4/NF-κB Signaling Pathway in Bovine Mammary Epithelial Cells

Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid

A Review on Dietary Flavonoids as Modulators of the Tumor Microenvironment

Contact Info

Product

Resources

About