Protective effect of Opuntia ficus - indica L. cladodes against UVA-induced oxidative stress in normal human keratinocytes

Petruk, Ganna; Lorenzo, Flaviana Di; Imbimbo, Paola; Silipo, Alba; Bonina, Andrea; Rizza, Luisa; Piccoli, Renata; Monti, Daria Maria; Lanzetta, Rosa

doi:10.1016/j.bmcl.2017.10.043

Cited by 40 publications

(32 citation statements)

References 27 publications

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“…Indeed, the preincubation of UVA-irradiated BALB/3T3 cells with açai phenolic compounds interfered with ROS production and kept GSH levels and lipid peroxidation comparable to normal cellular levels [ 74 ]. In another paper, we showed the beneficial effects of water extracts from Opuntia ficus-indica L. cladodes on human keratinocytes [ 91 ]. In particular, the phenolic compounds eucomic and piscidic acids were found to be the main active molecules responsible for the protection of keratinocytes against the UVA-induced oxidative stress and apoptosis [ 91 ].…”

Section: Fruits and Vegetables As Powerful Sources Of Antiaging Anmentioning

confidence: 99%

“…In another paper, we showed the beneficial effects of water extracts from Opuntia ficus-indica L. cladodes on human keratinocytes [ 91 ]. In particular, the phenolic compounds eucomic and piscidic acids were found to be the main active molecules responsible for the protection of keratinocytes against the UVA-induced oxidative stress and apoptosis [ 91 ].…”

Section: Fruits and Vegetables As Powerful Sources Of Antiaging Anmentioning

confidence: 99%

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Antioxidants from Plants Protect against Skin Photoaging

Petruk

Giudice

Rigano

et al. 2018

Oxidative Medicine and Cellular Longevity

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192

133

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Exposure to UV light triggers the rapid generation and accumulation of reactive oxygen species (ROS) in skin cells, with consequent increase in oxidative stress and thus in photoaging. Exogenous supplementation with dietary antioxidants and/or skin pretreatment with antioxidant-based lotions before sun exposure might be a winning strategy against age-related skin pathologies. In this context, plants produce many secondary metabolites to protect themselves from UV radiations and these compounds can also protect the skin from photoaging. Phenolic compounds, ascorbic acid and carotenoids, derived from different plant species, are able to protect the skin by preventing UV penetration, reducing inflammation and oxidative stress, and influencing several survival signalling pathways. In this review, we focus our attention on the double role of oxidants in cell metabolism and on environmental and xenobiotic agents involved in skin photoaging. Moreover, we discuss the protective role of dietary antioxidants from fruits and vegetables and report their antiaging properties related to the reduction of oxidative stress pathways.

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Section: Fruits and Vegetables As Powerful Sources Of Antiaging Anmentioning

confidence: 99%

Section: Fruits and Vegetables As Powerful Sources Of Antiaging Anmentioning

confidence: 99%

Antioxidants from Plants Protect against Skin Photoaging

Petruk

Giudice

Rigano

et al. 2018

Oxidative Medicine and Cellular Longevity

Self Cite

192

133

View full text Add to dashboard Cite

show abstract

“…selected because they were suggested to be effective at impacting either skin inflammation or direct expression of IL-1β in keratinocytes. [45][46][47][48] Prior to examining the polysaccharide blend, they were examined for their cytotoxicity to keratinocytes [data not shown]. The blend was then developed by carefully mixing the polysaccharides such that topical applications on keratinocytes in the range of 1-3% would provide each polysaccharide at a dose below its demonstrated effective but non-cytotoxic level.…”

Section: Initiating Nlrp Inflammasome Activation: Chemical Sourcesmentioning

confidence: 99%

<p>In vitro expression of NLRP inflammasome-induced active Caspase-1 expression in normal human epidermal keratinocytes (NHEK) by various exogenous threats and subsequent inhibition by naturally derived ingredient blends</p>

Gruber¹,

Holtz²

2019

JIR

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Background: The discovery of the nod-like receptor protein (NLRP) inflammasomes in 2002 has led to the rapid identification of these unique cellular proteins as key targets for studies on innate inflammation pathways. The NLRP inflammasomes have been shown to be expressed in normal human epidermal keratinocytes (NHEK) and human dermal fibroblasts (HDF). NLRP inflammasomes in keratinocytes are interesting as these skin cells are the first living cells in the skin to contact external exogenous threats such as UV energy, chemicals, physical trauma, and bacteria and viruses. Activation of the NLRP Inflammasomes by exogenous threats results in the release of active Caspase-1 (ACasp-1), a key protease enzyme, which targets inactive forms of IL-1β, IL-18 as well as IL-1α and IL-33. Purpose: This article discusses efforts to examine the release of active Caspase-1 from NHEKs activated by various exogenous threats including UVB energy, ATP, Nigericin and Urban Dust. The work further examines if, after inflammasome activation and Caspase-1 release, certain naturally derived botanical ingredients known to have anti-inflammatory effects can function to inhibit upregulation of active Caspase-1. Methods: NHEK were treated with various doses of UVB, ATP and Nigericin and with a single dose of Urban Dust. ACasp-1 expression was measured after 3 and 20 hours using the Promega Caspase Glo-1 bioluminescent assay. After confirmation that 60 mJ/cm 2 of UVB and 5mM of ATP were effective to activate NHEK ACasp-1 release after 20 hrs, these conditions were employed to examine the influence of three botanical blends of ingredients on their ability to inhibit ACasp-1 expression. Results: Initial results demonstrate that NHEKs can be activated to release active Caspase-1 by ATP and UVB, but not by Nigericin or Urban Dust. In addition, it was unexpectedly found that, while ATP and UVB activated NHEKs, the release of ACasp-1-did not happen within the first 3 hours after exposure but did become significant after 20 hours. Additional results indicate that a blend of polysaccharides and two blends of antioxidants, one oilsoluble and the other water-soluble, known for their anti-inflammatory effects, can reduce expression of active Caspase-1 in activated NHEKs when applied extracellularly. Conclusion: Expression of NLRP activated release of ACasp-1 was found to be influenced by UVB and ATP but not by Nigericin or Urban Dust. The effects were also time dependent. Several botanical extract blends were found to reduce ACasp-1 expression in previously activated NHEKs. Links between these inflammatory effects and processes of cellular inflammaging are discussed.

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“…In Human keratinocytes Opuntia exerted anti-oxidant activities suggesting its beneficial role in treating barrier-disrupted skin disorders (Nakahara et al, 2015). Moreover, similar antioxidant beneficial effects were observed in UVA stressed keratinocytes (Petruk et al, 2017).…”

Section: Introductionmentioning

confidence: 79%

Natural dietary antioxidants containing flavonoids modulate keratinocytes physiology: In vitro tri-culture models

Bassino

Gasparri

Munaron

2019

Journal of Ethnopharmacology

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ETHNOPHARMACOLOGICAL RELEVANCE: Several traditional medicinal herbs are widely used for dermatologic and cosmetic preparations. The beneficial skin repair activity is detected in various phases of wound-healing process, such as cell-cell, cell-matrix interactions or collagen synthesis. AIM OF THE STUDY: The study assessed the effects of Opuntia ficus-indica (L.) Mill. (Opuntia) and Milk Thistle (MT) (Silybum marianum (L.) Gaerth) on adult keratinocytes (HaCaT) functioning under basal condition or in the presence of mechanical damage (wounded cells). MATERIALS AND METHODS: The role of the natural compounds was tested on HaCaT grown in mono-culture and tri-culture configurations. In tri-cultures models, HaCaT were treated with the conditioned media (CM) obtained by Human Normal Dermal Fibroblast (NHDF) and Human Dermal Microvascular Endothelial cells (HMVEC) co-cultures. Specifically, were tested cell viability, oxidative stress mechanisms (cytokines release and lipid peroxidation) and cellular remodelling (growth factors release or metalloproteinase modulation). Moreover, the migratory potential of HaCaT was analysed by the use of wound healing in vitro assay. RESULTS: Opuntia and MT differently modified the metabolism (EGF, MMP-9), and the migratory properties of HaCaT both under physiological conditions or upon mechanical damage (wounded cells). Moreover, both compounds modulated HaCaT response to oxidative stress. The response to the natural compounds were modified, and in some cases potentiated, in tri-culture configuration systems. CONCLUSIONS: The data demonstrated that in vitro tri-culture approach is suitable to characterize the role of natural compounds on the complex communication between dermalepidermal cellular components and microvascular endothelium. Specifically, Opuntia and MT are good alternatives to synthetic compounds in skin repair promotion.

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Protective effect of Opuntia ficus - indica L. cladodes against UVA-induced oxidative stress in normal human keratinocytes

Cited by 40 publications

References 27 publications

Antioxidants from Plants Protect against Skin Photoaging

Antioxidants from Plants Protect against Skin Photoaging

<p>In vitro expression of NLRP inflammasome-induced active Caspase-1 expression in normal human epidermal keratinocytes (NHEK) by various exogenous threats and subsequent inhibition by naturally derived ingredient blends</p>

Natural dietary antioxidants containing flavonoids modulate keratinocytes physiology: In vitro tri-culture models

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