Protective Effect of Polydatin on Jejunal Mucosal Integrity, Redox Status, Inflammatory Response, and Mitochondrial Function in Intrauterine Growth-Retarded Weanling Piglets

Zhang, Hao; Chen, Yanan; Li, Yue

doi:10.1155/2020/7178123

Cited by 21 publications

(30 citation statements)

References 56 publications

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“…Another interesting observation is that the percentage of goblet cells did not increase in the IUGR animals between postnatal day 7 and 14, as is the case in their normal littermates, which further confirms that the development of the gut mucosa is delayed in the IUGR piglets. As recently suggested by Zhang and co-workers [ 22 ], this delay probably ends after weaning. The decreased percentage of goblet cells in the neonatal period leads to a decreased secretion of mucins, the major product of goblet cells, thereby enhancing the likelihood of an increase in bacterial adhesion to the intestinal epithelium [ 10 , 20 , 22 ].…”

Section: Discussionsupporting

confidence: 56%

“…As recently suggested by Zhang and co-workers [ 22 ], this delay probably ends after weaning. The decreased percentage of goblet cells in the neonatal period leads to a decreased secretion of mucins, the major product of goblet cells, thereby enhancing the likelihood of an increase in bacterial adhesion to the intestinal epithelium [ 10 , 20 , 22 ]. Huang et al found that 12 h after birth, IUGR and normal neonates already display distinct differences in bacterial colonization, which, along with the delayed maturation of enterocytes, may constitute an early factor promoting enteritis in IUGR animals [ 20 ].…”

Section: Discussionsupporting

confidence: 56%

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Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets

Olszewski

Zabielski

Skrzypek

et al. 2021

Animals

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Intrauterine growth restricted (IUGR) piglets are born at term but have low birth mass and a characteristic shape of the head. Impaired general condition, especially in intestinal function, leads to an increase in the occurrence of diarrhoea and high mortality in the first days of life. So far, the mechanical and immunological gut barrier functions in IUGR are poorly understood. The aim of this study was to microscopically evaluate the early postnatal changes in the gut mucosa occurring in IUGR piglets. Whole-tissue small intestine samples were collected from littermate pairs (IUGR and normal) on postnatal day (PD) 7, 14 and 180 and analysed by light microscopy. We found that in the IUGR piglets, the percentage of intraepithelial leukocytes was reduced in the duodenum on PD 7, but it increased in the proximal and middle jejunum both on PD 7 and PD 14, which suggested the development of an inflammatory process. The number of goblet cells was also reduced on PD 14. The average size of the Peyer’s patches in the distal jejunum and ileum showed significant reduction on PD 7 as compared to normal pigs; however, on PD 14, it returned to normal. On PD 180, we did not find any differences in the measured parameters between the IUGR and the normal pigs. In conclusion, we found that in one-week-old IUGR pig neonates, the gut barrier and the immune system structures display signs of retarded development but recover within the second postnatal week of life.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionsupporting

confidence: 56%

Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets

Olszewski

Zabielski

Skrzypek

et al. 2021

Animals

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show abstract

“…It is released into the blood and enters the intestinal tract when the intestinal mucosa is injured or necrotic. Therefore, plasma DAO activity may reflect intestinal mucosal integrity [ 23 ]. In this study, DSS markedly augmented the plasma LPS abundance and DAO activity compared to the control treatment.…”

Section: Resultsmentioning

confidence: 99%

“…In healthy individuals, the intact intestinal mucosa acts as a barrier in preventing entry of LPS and DAO into the portal circulation [ 22 , 23 ]. Chronic colitis in IBD consistently demonstrates increased intestinal permeability.…”

Section: Discussionmentioning

confidence: 99%

Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway

Wang

Huang

Tan

et al. 2022

Oxidative Medicine and Cellular Longevity

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Inflammatory bowel diseases (IBDs) constitute a group of chronic intestinal conditions prominently featuring deranged metabolism. Effective pharmacological treatments for IBDs are lacking. Isosteviol sodium (STV-Na) exhibits anti-inflammatory activity and may offer therapeutic benefits in chronic colitis. However, the associated mechanism remains unclear. This study is aimed at exploring the therapeutic effects of STV-Na against chronic colitis in terms of metabolic reprogramming and macrophage polarization. Results show that STV-Na attenuated weight loss and colonic pathological damage and restored the hematological and biochemical parameters in chronic colitis mice models. STV-Na also restored intestinal permeability by increasing the goblet cell numbers, which was accompanied by lowered plasma lipopolysaccharide and diamine oxidase levels. Metabolomic analysis highlighted 102 candidate biomarkers and 5 vital pathways that may be crucial in the potential pharmacological mechanism of STV-Na in regulating intestinal inflammation and oxidative stress. These pathways were glycerophospholipid metabolism, phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, the pentose phosphate pathway, and phosphonate and phosphinate metabolism. Furthermore, STV-Na significantly decreased M1 macrophage polarization in the spleen and colon. The mRNA and protein levels of IL-1β, TNF-α, and NF-κB/p65 in colonic tissue from the colitis mice were decreased after the STV-Na treatment. Overall, STV-Na could alleviate chronic colitis by suppressing oxidative stress and inflammation levels, reprogramming the metabolic profile, inhibiting macrophage polarization, and suppressing the NF-κB/p65 signaling pathway. STV-Na remains a promising candidate drug for treating IBDs.

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“…Polydatin may alleviate mucosal oxidative stress and immunological damage and may improve the intestinal epithelium barrier. 46,47…”

Section: Discussionmentioning

confidence: 99%

Combined Network Pharmacology, Molecular Docking, and Experimental Verification Approach to Investigate the Potential Mechanisms of Polydatin Against COVID-19

Wang

Qin

Zhai

2022

Natural Product Communications

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Background: Coronavirus disease 2019 (COVID-19) has posed a serious threat to human health and there is an urgent need for drug development. In this study, we explored the potential mechanisms underlying the efficacy of polydatin against COVID-19. Methods: A combined approach of network pharmacology, molecular docking, and experimental verification were employed in this study. Potential targets of polydatin for treating COVID-19 were obtained from multiple drug and disease databases. Protein–protein interaction and enrichment analyses were performed to predict the potential mechanism of action of polydatin against COVID-19. The binding potential of polydatin and key targets was evaluated through molecular docking. Furthermore, experimental methods including flow cytometry and luciferase assay were used to validate the results of computational analyses. Results: The main diseases identified as polydatin targets included metabolic diseases, lung diseases, inflammation, infectious diseases, and tumors. Polydatin may be used to treat COVID-19 through interventions that alter the immune and inflammatory responses, including IL-17 signaling pathway, T-cell activation, cytokines and inflammatory response, lipopolysaccharide-mediated signaling pathway, as well as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) innate immunity evasion and cell-specific immune response. Polydatin can potentially bind to the target proteins related to COVID-19, such as SARS-CoV-2 Mpro, RdRp, and human angiotensin-converting enzyme 2 (ACE2), while directly exerting its regulatory or therapeutic functions. The experimental results showed that polydatin decreased the infectivity of the SARS-CoV-2 spike pseudovirus in HEK293T-ACE2 cells. Accordingly, polydatin may retard the entry of SARS-CoV-2 into cells by competitively binding to human ACE2. Conclusion: The potential targets and signaling pathways of polydatin against COVID-19 were preliminarily identified. The findings may benefit the development and application of polydatin as a treatment for COVID-19.

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Protective Effect of Polydatin on Jejunal Mucosal Integrity, Redox Status, Inflammatory Response, and Mitochondrial Function in Intrauterine Growth-Retarded Weanling Piglets

Cited by 21 publications

References 56 publications

Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets

Differences in Intestinal Barrier Development between Intrauterine Growth Restricted and Normal Birth Weight Piglets

Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway

Combined Network Pharmacology, Molecular Docking, and Experimental Verification Approach to Investigate the Potential Mechanisms of Polydatin Against COVID-19

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