Protective effect of pristimerin against LPS-induced acute lung injury in mice

Shaaban, Ahmed A.; El‐Kashef, Dalia H.; Hamed, Mohamed F.; El‐Agamy, Dina S.

doi:10.1016/j.intimp.2018.03.033

Cited by 41 publications

(39 citation statements)

References 39 publications

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“…To quantify the magnitude of pulmonary edema, the lung W/D ratio was evaluated. In line with previous investigations (Ammar et al, 2013;Shaaban et al, 2018), LPS caused marked pulmonary edema. On the other hand, VAC decreased the lung W/D ratio, indicating that VAC could inhibit the leakage of serous fluid into the lung tissue and attenuate the development of pulmonary edema.…”

Section: Discussionsupporting

confidence: 93%

“…LDH activity in the BALF, an indicator of cellular damage and death, increased following respiratory infection (Ward, Till, Hatherill, Annesley, & Kunkel, 1985). It was reported previously that LDH is elevated in the lung following LPS challenge (Ammar et al, 2013;Shaaban et al, 2018). Consistent with these studies, our results showed that LDH level in the lung BALF was increased by LPS administration compared with the healthy control group, which was inhibited by VAC pretreatment.…”

Section: Discussionsupporting

confidence: 91%

“…The animals were treated according to the following regimen: control group where mice were given the vehicle once daily for 5 days; LPS group where mice received a single intraperitoneal injection of LPS (10 mg/kg); and VAC‐treated groups where the animals were administered VAC (50 and 100 mg/kg, po) for 5 days before the LPS injection. The dose of LPS was selected based on a previously reported study (Shaaban, El‐Kashef, Hamed, & El‐Agamy, ).…”

Section: Methodsmentioning

confidence: 99%

“…Malondialdehyde (MDA) content is eventually used as an indicator of lipid peroxidation while superoxide dismutase (SOD) activity and reduced glutathione (GSH) content were assessed as the indices of the antioxidant capacity of the lung (Shaaban et al, ). These parameters were estimated in the supernatants of the lung homogenates.…”

Section: Methodsmentioning

confidence: 99%

“…The dose of LPS was selected based on a previously reported study (Shaaban, El-Kashef, Hamed, & El-Agamy, 2018).…”

Section: Experimental Designmentioning

confidence: 99%

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Vitex agnus‐castussafeguards the lung against lipopolysaccharide‐induced toxicity in mice

et al. 2018

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Vitex agnus‐castus (VAC, Verbenaceae) is widely used in Chinese traditional medicine as an antiinflammatory agent. This study aimed to explore the efficacy of the VAC extract to protect against lipopolysaccharide (LPS)‐induced acute lung injury. The results have shown that VAC had a potent protective activity against LPS‐induced acute lung damage. It significantly decreased pulmonary edema as there was a significant decrease in lung wet/dry ratio and in protein content. VAC also decreased the lactate dehydrogenase's activity in the bronchoalveolar fluid. VAC ameliorated LPS‐induced inflammatory cells infiltration into the lung tissue and reversed the histopathological lesions of the lung. Furthermore, VAC counteracted LPS‐induced oxidative stress as it attenuated the lipid peroxidation marker, malondialdehyde, in the lung. VAC increased the antioxidant activity as evident by elevated superoxide dismutase activity and increased reduced glutathione content in the lung tissue. Collectively, VAC has a protective activity against LPS‐induced acute lung damage through its antioxidant potential. Practical applications Vitex agnus‐castus has been used in various traditional medicines for treating various ailments as digestive complains, acne, rheumatic pains, menstrual irregularities, premenstrual syndrome, infertility, and hyperprolactinemia. Its leaves are used as a spice and the fruits are used as a substitute for pepper. VAC food supplements are used by women against psychic and somatic premenstrual symptoms. The findings of this study can demonstrate the potent protective activity of the VAC extract against LPS‐induced acute lung damage due to its antioxidative effects. Therefore, VAC could be developed as a health functional food to improve acute lung damage and many diseases caused by oxidative damage.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The dose of LPS was selected based on a previously reported study (Shaaban, El-Kashef, Hamed, & El-Agamy, 2018).…”

Section: Experimental Designmentioning

confidence: 99%

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Vitex agnus‐castussafeguards the lung against lipopolysaccharide‐induced toxicity in mice

et al. 2018

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Physical exercise prevents alterations in purinergic system and oxidative status in lipopolysaccharide‐induced sepsis in rats

Miron

Bottari

Assmann

et al. 2018

J of Cellular Biochemistry

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Sepsis is a generalized infection that involves alterations in inflammatory parameters, oxidant status, and purinergic signaling in many tissues. Physical exercise has emerged as a tool to prevent this disease because of its anti-inflammatory and antioxidant properties. Thus, in this study, we investigated the effects of physical exercise on preventing alterations in purinergic system components, oxidative stress, and inflammatory parameters in lipopolysaccharide (LPS)-induced sepsis in rats. Male Wistar rats were divided into four groups: control, exercise (EX), LPS, and EX+LPS. The resisted physical exercise was performed for 12 weeks on a ladder with 1 m height. After 72 hours of the last exercise session, the animals received 2.5 mg/kg of LPS for induction of sepsis, and after 24 hours, lungs and blood samples were collected for analysis. The results showed that the exercise protocol used was able to prevent, in septic animals: (1) the increase in body temperature; (2) the increase of lipid peroxidation and reactive species levels in the lung, (3) the increase in adenosine triphosphate levels in serum; (4) the change in the activity of the enzymes ectonucleotidases in lymphocytes, partially; (5) the change in the density of purinergic enzymes and receptors in the lung, and (6) the increase of IL-6 and IL-1β gene expression. Our results revealed the involvement of purinergic signaling and oxidative damage in the mechanisms by which exercise prevents sepsis aggravations. Therefore, the regular practice of physical exercise is encouraged as a better way to prepare the body against sepsis complications.

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Gossypin exert lipopolysaccharide induced lung inflammation via alteration of Nrf2/HO‐1 and NF‐κB signaling pathway

Huang

Wang²,

Hussain

et al. 2023

Environmental Toxicology

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Acute Lung Injury (ALI) is a critical medical condition that induces the injury into the lung tissue, resulting in decreased the oxygen levels in the circulation and finally causes the respiratory failure. In this study, we try to made effort for scrutinized the preventive effect of gossypin against lipopolysaccharide (LPS) induced lung inflammation and explore the underlying mechanism. LPS (7.5 mg/kg) was used for induction the lung inflammation in the rats and rats were received the oral administration of gossypin (5, 10 and 15 mg/kg). The wet to dry weight lung ratio and lung index were estimated. The bronchoalveolar lavage fluid (BALF) were collected to determination the inflammatory cells, total protein, macrophages and neutrophils. ELISA kits were used for the estimation of antioxidant, inflammatory cytokines, inflammatory parameters, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) parameters. Finally, we used the lung tissue for scrutinize the alteration in the lung histopathology. Gossypin treatment significantly (p < .001) reduced the W/D ratio of lung tissue and lung index. Gossypin significantly (p < .001) decreased the total cells, neutrophils, macrophages and total protein in BALF. It is also altered the level of inflammatory cytokines, antioxidant and inflammatory parameters, respectively. Gossypin improved the level of Nrf2 and HO-1 at dose dependent manner. Gossypin treatment remarkably enhance the ALI severity via balancing the structural integrity of lung tissue, decrease the thickness of the alveolar wall, decline the pulmonary interstitial edema, and number of inflammatory cells in the lung tissue. Gossypin is a

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Protective effect of pristimerin against LPS-induced acute lung injury in mice

Cited by 41 publications

References 39 publications

Vitex agnus‐castussafeguards the lung against lipopolysaccharide‐induced toxicity in mice

Vitex agnus‐castussafeguards the lung against lipopolysaccharide‐induced toxicity in mice

Physical exercise prevents alterations in purinergic system and oxidative status in lipopolysaccharide‐induced sepsis in rats

Gossypin exert lipopolysaccharide induced lung inflammation via alteration of Nrf2/HO‐1 and NF‐κB signaling pathway

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