Protective effect of propranolol on ethanol-induced gastric lesions in mice

Bhandare, Padma N.; Diniz-D'Souza, Rosa; Mainker, Avinash; Dhume, V. G.

doi:10.1016/0014-2999(90)94144-m

Cited by 13 publications

(10 citation statements)

References 18 publications

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“…These results were similar to that of [14] who mentioned that in case of gastric ulceration, the nearby blood vessels appeared dilated and congested.…”

Section: Discussionsupporting

confidence: 90%

Protective Effect of Ascorbic Acid and Ranitidine on the Indomethacin Induced Gastric Injury in Adult Albino Rats; Light and Electron Microscopy

Shalaby¹

2016

IJCDA

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Abstract:Indomethacin is a non-steroidal anti-inflammatory drug, used in the treatment of many inflammatory diseases.The major side effect limiting its clinical use is the gastric damages. The aim of this work was to study the possible protective effects of vitamin c and ranitidine on the gastric lesion induced by indomethacin in adult albino rats. Forty adult albino rats were divided into five equal groups: The control group, indomethacin group, indomethacin plus vitamin c, indomethacin plus ranitidine, and indomethacin plus both vitamin c and ranitidine. In the control group, the rats received normal daily diet for 10 days. In the indomethacin group, the rats were treated with indomethacin for 10 consecutive days. In indomethacin plus vitamin c group, the rats were treated with indomethacin as the second group, in addition to vitamin c, 5 minutes prior to the indomethacin for 10 days. In the fourth group, the rats were treated with indomethacin as the second group, and then received ranitidine 5 min prior to the indomethacin, for 10 consecutive days. The last group received the indomethacin as the second group, and then received vitamin c and ranitidine, 5 min prior to the indomethacin, daily for 10 days. After 10 days from the treatment, all the rats were anaesthetized, and the specimens of the stomach were prepared for light and electron microscopies. Indomethacin produced erosions and ulcerations of the gastric mucosa. There are focal areas of degeneration and inflammatory cell infiltration in the lamina propria, the gastric gland cells showed vacuolations, degeneration and disappearance of their nuclei. It also induced dilatation and congestion of the blood capillaries in the submucosa of the stomach, also the surface mucin layer was decreased in its thickness. Electron microscopic examination showed that: Indomethacin induced degeneration and vacuolations of the gastric gland cells, with degeneration of their mitochondria and nuclei. Chief cells showed abnormalities in their zymogenic granules which appeared with dark central part and pale periphery. Administration of vitamin c and ranitidine prior to the indomethacin intake reduced the pathogenic changes of the gastric wall, with slight return to the picture of the control group.

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“…These results were similar to that of [14] who mentioned that in case of gastric ulceration, the nearby blood vessels appeared dilated and congested.…”

Section: Discussionsupporting

confidence: 90%

Protective Effect of Ascorbic Acid and Ranitidine on the Indomethacin Induced Gastric Injury in Adult Albino Rats; Light and Electron Microscopy

Shalaby¹

2016

IJCDA

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“…Necropsy of the alcohol-fed mice on the day of sacrifice revealed that the animals had experienced intestinal hemorrhage during the alcohol treatment phase. Consumption of alcohol is known to result in gastric hemorrhagic lesions as well as mucosal injury to the small intestine (Bhandare et al, 1990;Bode and Bode, 2003). However supplementing the alcohol containing liquid diet with chow abolished the intestinal hemorrhage.…”

Section: Discussionmentioning

confidence: 99%

Supplementing the liquid alcohol diet with chow enhances alcohol intake in C57 BL/6 mice

Anji

2008

Drug and Alcohol Dependence

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Background-Administration of alcohol-containing liquid diet is associated with body weight loss in rodents.Aim-of this study was to modify the alcohol-containing liquid diet paradigm to reduce the body weight loss in mice during the alcohol treatment period.Methods-Two sets of animals (Chow and No Chow groups) were exposed to chronic alcohol with a step-wise increase of alcohol in the diet. One set of control and alcohol exposed animals (Chow group) received chow during alcohol treatment. Food intake and body weight was measured every 24h. Level of intoxication was determined by measuring blood alcohol levels. Alcohol dependence of mice was determined by handling-induced convulsions (HIC) scoring. Chronic alcohol-mediated effects on brain and liver were examined.Results-Body weight loss was attenuated in chronic alcohol exposed mice in Chow group as compared to No Chow group. Chow group mice consumed higher amounts of alcohol diet resulting in higher blood alcohol levels. Brain NMDAR1 protein levels and liver Cyp2E1 levels were significantly enhanced in chronic alcohol-exposed mice in Chow and No Chow groups suggesting that known medical consequences of alcohol are not interfered with in our modified alcohol treatment paradigm. HIC in Chow and No Chow group mice peaked between 3h and 5h after alcohol withdrawal. However, the severity of alcohol withdrawal was greater in Chow group mice.Conclusions-Supplementing alcohol diet with chow not only attenuated body weight loss associated with alcohol intake in mice but also resulted in higher consumption of alcohol diet and higher blood alcohol levels. KeywordsC57 BL/6 mice; chronic alcohol treatment; handling-induced convulsions; NMDA R1 subunit; Cyp 2E1 enzyme activity

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“…The stomach and duodenum were removed to evaluate ethanol-induced gastroduodenal lesions. In previous reports using 90% or absolute ethanol, mucosal injury was evaluated 60 minutes after ethanol administration (Bhandare et al, 1990;Melchiorri et al, 1997;Tsuji et al, 1993). Because we used 60% ethanol as well as 99.5% ethanol, a longer duration (90 minutes) than that used in the previous reports was selected before we observed the tissues for mucosal injury.…”

Section: Treatmentmentioning

confidence: 99%

Cytoprotection by Metallothionein Against Gastroduodenal Mucosal Injury Caused by Ethanol in Mice

Takano

Satoh

Shimada

et al. 2000

Laboratory Investigation

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SUMMARY:Metallothionein (MT) is a small, cysteine-rich protein that can act as a free radical scavenger at least in vitro. To test the hypothesis that MT participates in gastroduodenal cytoprotection, we studied sensitivity to gastroduodenal mucosal injury caused by ethanol in MT-null mice that have null mutations in MT-I and MT-II genes. MT-null mice and wild-type mice were orally treated with ethanol (60% or 99.5%, 0.2 ml/mouse). The macroscopic gastric lesion indices were significantly higher in MT-null mice than in wild-type mice 90 minutes after ethanol treatment. Histopathological examination in ethanol-treated MT-null mice showed vacuolar degeneration, necrosis of the epithelial cells, and hemorrhage throughout the tunica mucosa. Moreover, the duodenum also showed morphologic changes, including marked degeneration and coagulative necrosis of the entire villi, desquamation of the degenerated epithelial cells, and hemorrhage. In contrast, histopathologic changes were less prominent in the wild-type mice treated with ethanol. MT was not detected either in the stomach or duodenum of MT-null mice, whereas gastric and duodenal zinc contents were not significantly different between MT-null mice and wild-type mice. These results provide direct evidence that intrinsic MT plays a cytoprotective role in gastroduodenal mucosal injury caused by ethanol. (Lab Invest 2000, 80:371-377).

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Protective effect of propranolol on ethanol-induced gastric lesions in mice

Cited by 13 publications

References 18 publications

Protective Effect of Ascorbic Acid and Ranitidine on the Indomethacin Induced Gastric Injury in Adult Albino Rats; Light and Electron Microscopy

Protective Effect of Ascorbic Acid and Ranitidine on the Indomethacin Induced Gastric Injury in Adult Albino Rats; Light and Electron Microscopy

Supplementing the liquid alcohol diet with chow enhances alcohol intake in C57 BL/6 mice

Cytoprotection by Metallothionein Against Gastroduodenal Mucosal Injury Caused by Ethanol in Mice

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