Protective Effect of Quercetin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

Yu, Sha; Long, Hui; Lyu, Qifeng; Zhang, Qinhua; Zhou, Yan; Hongxing, Liang; Chai, Weidong; Kuang, Yanping; Qian, Cong

doi:10.1371/journal.pone.0089520

Cited by 42 publications

(49 citation statements)

References 43 publications

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“…In agreement to our results, Yu et al (2014) reported that the low-dose quercetin exposure (1 mM) provided mild protection by an increase in the percentage of embryos that reached the morula stage. With a concentration of quercetin up to 5 mM, blastocyst formation was recovered to levels of the control group.…”

Section: Discussionsupporting

confidence: 93%

“…Interestingly, quercetin has contradictory roles -prooxidant or antioxidant depending on the experimental situation and dose (Yu et al, 2014). It is documented that high concentration of ROS may result in oxidative damage which subsequently causes apoptotic cell death in various cell types (Bayir et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Yu et al (2014) investigated the effects of quercetin on the development of preimplantation embryos under oxidative stress and found that quercetin exposure highly intensifies the quality of H 2 O 2 -treated embryos, depicted by reduced apoptosis, increased blastocyst formation, and fewer incidences of fragmentation and developmental retardation. They concluded that this effect of quercetin on the development of preimplantation embryos may be connected with the antioxidant activity of quercetin in reduction of ROS levels in zygotes.…”

Section: Discussionmentioning

confidence: 99%

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Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Banihosseini

Novin

Nazarian

et al. 2018

Annals of Animal Science

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Quercetin is a natural flavonoid with strong antioxidant activity. In the present study, we evaluate the influence of different concentrations of quercetin (QT) on intracytoplasmic oxidative stress and glutathione (GSH) concentration, during in vitro maturation (IVM) and fertilization in mouse oocytes. IVM was carried out in the presence of control (QT0), 5 (QT5), 10 (QT10), and 20 (QT20) µg/mL of QT. Nuclear maturation, intracellular GSH and ROS content were evaluated following the IVM. In these oocytes, we subsequently evaluated the effect of QT supplementation on embryo development, including 2-cell, 8-cell, and blastocyst rate. The results of the present study showed that the supplementation of 10 µg/mL QT in maturation medium increased the number of MII oocytes. In addition, fertilization and blastocyst rate in QT10 treatment group were significantly higher in comparison to the other groups, and elevated the amount of intracellular GSH content compared to other QT concentrations and control groups. The intracellular ROS level was the lowest among oocytes matured in Q5 and Q10 treatment groups. This result suggested that quercetin dose-dependently improves nuclear maturation and embryo development, via reducing intracytoplasmic oxidative stress in mature oocyte. Key words: in vitro maturation, quercetin, oxidative stress, glutathioneModern assisted reproductive technologies (ART) use ovarian stimulation to expand the quantity of accessible oocytes and the result. However, the utilization of ovarian stimulation builds the patients' cost and suffering, and is related with side effects including ovarian hyperstimulation syndrome (OHSS) and potential cancer risk. The recovery of immature oocytes followed by in vitro maturation (IVM) and Unauthenticated Download Date | 5/12/18 10:05 AM

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Banihosseini

Novin

Nazarian

et al. 2018

Annals of Animal Science

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“…For example, quercetin protects Caco-2 cells against mitochondrial damage [39]. It is also reported that quercetin provided stable mitochondrial function by modulating cellular antioxidant defense and decreasing ROS in mouse zygotes [25]. In this study, quercetin showed strong antioxidant effect against superoxide generation in mitochondria.…”

Section: Discussionsupporting

confidence: 49%

“…Quercetin treated cell groups showed a dramatic fall in viability over time in a dose dependent manner ( Figure 3A). However, it is worth noting that the concentration of quercetin here was much higher than the usual concentrations reportedly used in other studies [16,25]. Hence, the cytotoxic effect of quercetin for cells reported in this study can be regarded excess in amount.…”

mentioning

confidence: 50%

Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO2 Nanoparticles in Swiss 3T3 Albino Mouse Fibroblast Cells

Birinci¹,

Niazi²,

Başağa³

2017

J Nanomed Nanotechnol

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Living organisms are continuously exposed to a wide variety of oxidative radicals. Therefore, there is a constant demand for exogenous antioxidant supply. In this regard, one of these exogenous antioxidants with well established potency, quercetin has been utilized in various formulations. Yet due to its poor water solubility, its extensive applications are limited so far. In this study, TiO 2 nanoparticles (TiO 2 -NPs) were employed to improve the cellular penetration of quercetin and to maximize its antioxidant effects on swiss 3T3 albino mouse fibroblast cells. Surfaces of TiO 2 -NPs were modified with Polyethylene glycol (PEG) that enabled better dispersion and enhanced biocompatibility. Toxicity of quercetin and quercetin loaded TiO 2 NPs (QL-TiO 2 -NPs) were evaluated in terms of cell morphology and measured with cell viability assay. For an in-depth cell viability analysis, key markers of apoptosis were investigated by immunoblotting analysis. As an indicator of apoptotic cell death, cleavage of caspase 3 (Cas 3) and poly (ADP-ribose) polymerase proteins (Parp) were detected in quercetin treated cells. Antioxidant capacity of quercetin in the form of QL-TiO 2 -NPs was measured in the cells in which generation of reactive oxygen species (ROS) and superoxide was induced by pyocyanin. Quantitative ROS measurements were confirmed with confocal microscopy. Further mechanistic insight on upregulation of NF-E2 related factor 2 (NRF2) pathway via QL-TiO 2 -NPs was also provided in an effort to validate its antioxidant defense. Target enzymes of NRF2, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxydoreductase1 (NQO1) and superoxide dismutase1 (SOD1) expressions were increased in the model proposed. QL-TiO 2 -NPs allowed high bioavaliability of quercetin concentration and stability in the cell with maximum antioxidant capacity against formation of ROS without any cytotoxicity. Overall, this paper sheds light on how the efficiency of quercetin in a nanosystem can serve as a safe therapeutic candidate for breaking the vicious ROS cycle in the cell. Citation: Birinci Y, Niazi JH, Basaga H (2017) Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO 2 Nanoparticles in Swiss 3T3Albino Mouse Fibroblast Cells. J Nanomedic Nanotechnol S8: 001. doi:10.4172/2157-7439.S8-001Page 2 of 10 J Nanomedic Nanotechnol Nanotechnology: Challenges and Perspectives in Medicine ISSN: 2157-7439 JNMNT an open access journal 3T3 albino mouse fibroblast cells. Total cellular ROS and superoxide levels in treated cells were determined and compared with those of control cells. However, quercetin in a nanosystem shows maximum antioxidant activity by triggering NRF2 pathway, upregulating phase II antioxidant enzymes, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxydoreductase1 (NQO1) and superoxide dismutase1 (SOD1). In summary, our study demonstrated that quercetin loaded TiO 2 -NPs served as an effective nanosystem for delivering quercetin to swiss 3T3 albino mouse fibroblast cells in vitro and provided protection against oxida...

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Role of STAT signaling and autocrine action of chemokines during H₂O₂ induced HTR‐8/SVneo trophoblastic cells invasion

Banerjee

Malik

Malhotra

et al. 2018

Journal Cellular Physiology

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During pregnancy, regulated generation of reactive oxygen species (ROS) is important for activation of signaling pathways and placentation. In the current study, the effect of H O on invasion of HTR-8/SVneo cells, a human extravillous trophoblast cell line, is investigated. Treatment of HTR-8/SVneo cells for 24 hr with H O (25 µM) leads to a significant increase in invasion without affecting cell proliferation, viability, and apoptosis. Concomitantly, a significant increase in the matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of metalloproteinases-1 (TIMP-1) ratio is observed. Further, significant increase in phosphorylation of signal transducer and activator of transcription 1 (STAT-1) and STAT-3 (both at ser727 residue) is observed on treating HTR-8/SVneo cells with 25 µM of H O accompanied by an increase in the secretion of interleukin-8 (IL-8) and macrophage inflammatory protein-1β (MIP-1β). A significant decrease in H O -mediated invasion of HTR-8/SVneo cells and reduced expression of IL-8 and MIP-1β accompanied by decrease in MMP-9/TIMP-1 ratio are observed on inhibiting STAT-1 and STAT-3 by small interfering RNA (siRNA). Inhibition of STAT-1 activity by fludarabine and STAT-3 activity by Stattic also leads to a decrease in H O -mediated increase in HTR-8/SVneo cell invasion. Inhibition of IL-8 and MIP-1β by siRNA also leads to a significant decrease in both basal and H O -mediated invasion. Interestingly, inhibition of MIP-1β by siRNA leads to a significant reduction in H O -mediated increase in IL-8. However, no significant effect of IL-8 silencing on H O -mediated MIP-1β expression was observed. From the above results, it can be concluded that H O activates STAT signaling, MIP-1β & IL-8 secretion and increases MMP-9/TIMP-1 ratio leading to an increased invasion of HTR-8/SVneo cells without affecting their viability.

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Protective Effect of Quercetin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

Cited by 42 publications

References 43 publications

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO2 Nanoparticles in Swiss 3T3 Albino Mouse Fibroblast Cells

Role of STAT signaling and autocrine action of chemokines during H₂O₂ induced HTR‐8/SVneo trophoblastic cells invasion

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Protective Effect of Quercetin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

Cited by 42 publications

References 43 publications

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Quercetin improves developmental competence of mouse oocytes by reducing oxidative stress during in vitro maturation

Enhancing Antioxidant Cellular Defense by Using Quercetin Loaded TiO2 Nanoparticles in Swiss 3T3 Albino Mouse Fibroblast Cells

Role of STAT signaling and autocrine action of chemokines during H2O2 induced HTR‐8/SVneo trophoblastic cells invasion

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Role of STAT signaling and autocrine action of chemokines during H₂O₂ induced HTR‐8/SVneo trophoblastic cells invasion