Protective effect of recombinant Trichinella 53-kDa protein in sepsis and the effect on macrophages

Yang, Chuwei; Liu, Hui; Li, Xiangdong; Sui, Shaoguang; Liu, Yuanyuan

doi:10.3892/etm.2016.3354

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…These effects have several features in common with those reported for one of the most abundant secreted T. spiralisNUDT9 proteins A0A0V1ASF1 (also known as GP53/Ts53). It has been shown to suppress inflammatory responses in mouse models of sepsis (Yang et al, 2016) and colitis (Du et al, 2011) and prevent death in mice from systemic administration of LPS (Chen et al, 2016).…”

Section: Esps Are Enriched In Nudt9-like Nudix Hydrolasesmentioning

confidence: 99%

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Nash

Gregory²,

White³

et al. 2023

Front. Parasitol.

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The Trichinella genus contains parasitic nematodes capable of infecting a wide range of hosts including mammals, birds and reptiles. Like other helminths, T. spiralis secretes a complex mixture of bioactive molecules capable of modulating its immediate surroundings and creating a hospitable environment for growth, survival and ultimately transmission. The constitution of these excretory-secretory products (ESPs) changes depending on the tissue niche and the specific stage of parasite development. Unique to T. spiralis is a true intracellular stage wherein larvae develop inside striated myotubes. Remarkably, the parasite larvae do not destroy the host cell but rather reprogram it to support their presence and growth. This transformation is largely mediated through stage-specific secretions released into the host cell cytoplasm. In this study, we apply state of the art proteomics and computational approaches to elucidate the composition and functions of muscle-stage T. spiralis ESPs. Moreover, we define a recurring, upstream motif associated with the stichosome, the main secretory organ of this worm, and can be used to predict secreted proteins across experimentally less tractable T. spiralis life cycle stages.

show abstract

Section: Esps Are Enriched In Nudt9-like Nudix Hydrolasesmentioning

confidence: 99%

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Nash

Gregory²,

White³

et al. 2023

Front. Parasitol.

View full text Add to dashboard Cite

show abstract

“…These effects have several features in common with those reported for one of the most abundant secreted T. spiralis NUDT9 proteins A0A0V1ASF1 (also known as GP53/Ts53). It has been shown to suppress inflammatory responses in mouse models of sepsis [57] and colitis [58] and prevent death in mice from systemic administration of LPS [59]. Administration of recombinant GP53 results in the activation of M2 macrophages, perhaps via the modulation of PARP activity by GP53.…”

Section: Esps Are Enriched In Nudt9-like Nudix Hydrolasesmentioning

confidence: 99%

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Nash

Gregory

White

et al. 2022

Preprint

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The Trichinella genus contains parasitic nematodes capable of infecting a wide range of hosts including mammals, birds and reptiles. Like other helminths, T. spiralis secretes a complex mixture of bioactive molecules capable of modulating its immediate surroundings and creating a hospitable environment for growth, survival and ultimately transmission. The constitution of these excretory-secretory products (ESPs) changes depending on the tissue niche and the specific stage of parasite development. Unique to T. spiralis is a true intracellular stage wherein larvae develop inside striated myotubes. Remarkably, the parasite larvae do not destroy the host cell but rather reprogram it to support their presence and growth. This transformation is largely mediated through stage-specific secretions released into the host cell cytoplasm. In this study, we apply state of the art proteomics and computational approaches to elucidate the composition and functions of muscle-stage T. spiralis ESPs. Moreover, we define a commonly-occurring, upstream motif that we believe is associated with the stichosome, the main secretory organ of this worm, and can thus be used to predict secreted proteins across experimentally less tractable T. spiralis life cycle stages.

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Proteomics of Trichinella

Nagano

Khueangchiangkhwang

et al. 2021

Trichinella and Trichinellosis

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Protective effect of recombinant Trichinella 53-kDa protein in sepsis and the effect on macrophages

Cited by 3 publications

References 21 publications

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Large-scale proteomic analysis of T. spiralis muscle-stage ESPs identifies a novel upstream motif for in silico prediction of secreted products

Proteomics of Trichinella

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