Protective Effect of Resveratrol Against Morphine Damage to Kidneys of Mice

Abstract

Morphine produces free radicals and cause apoptosis in some cell. Resveratrol (RSV) is a stilbenoid, a type of natural phenol, and a phytoalexin produced by several plants in response to injury. 48 male mice were randomly assigned to 8 groups. In this study, various doses of RSV (2, 8 and 20 mg/kg) and RSV plus Morphine (2, 8 and 20 mg/kg) were administered intraperitoneally to male mice for 20 consequent days and weight of kidneys, biochemical characteristics, morphometric markers and blood serum nitric oxide level were studied. The results indicated that morphine administration significantly increased the mean diameter of glomerulus and distal and proximal convoluted tubule, Lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and nitric oxide levels compared to the saline group (P<0.05). However, RSV and RSV plus morphine in all doses significantly decreased glomeruli number and LDH, BUN, creatinine and nitric oxide levels compared to morphine groups (p<0.05). Thus, it seems that resveratrol improved kidney damages induced by morphine in mice.

Resveratrol attenuates malathion-induced renal damage by declining oxidative stress in rats

“…Sixth to eighth groups, resveratrol + malathion administration groups, in these groups, each animal received single dose (27 mg/kg) of malathion through gavage to induce kidney parameter damage, and then, they received 2, 8, and 20 mg/kg, respectively, of resveratrol intraperitoneally for 45 days at 10 am. [6,10]…”

“…The left kidney was removed for histological and morphometric examinations and the right ones for the MDA level estimations, in respect of the groups. [11] The tissue preparing and staining…”

“…At the end of tissue processing, the stained sections were assessed under Olympus BX-51T-32E01 research microscope connected to a DP12 camera with 3.34-million pixel resolution and Olysia Bio software (Olympus Optical Co., Japan). [10] The histological quantification…”

“…[9] Increased reactive oxygen species (ROS) can induce oxidative stress and consequently lipid oxidation. [10] Oxidative stress is able to induce DNA failure and inactivation of specific proteins, which is usually followed by the loss of biologic membranes. [11] Free radicals cause lipid peroxidation in the membrane, change enzymatic activity, and induce cell damage and necrosis by attacking unsaturated fatty acids and alkylating the protein groups and other cellular macromolecules.…”

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“…cancer activity 5 , anti-antioxidant activity 6 , antiinflammatory 7 , anti-diabetic and anti-glycating property 8,9,10 , antimicrobial, antiepileptic and hepatoprotective activity 11,12,13 , potential remedial action on dental and oral illness 14 , protection against cardiovascular health problem 15 , a potentate drug against neurogenerative disorders and brain damage 16 , a remedy for renal diseases including nephrolithiasis and renal damage 17 , protection against kidney damage due to morphine toxicity 18 and as a curative drug for stress-induced gastric ulcers 19 , have been reported for this medicinal plant. The objective of this study is to screen the phytochemicals and to evaluate the antibacterial potential of Nigella sativa L. seeds against ESBL positive Escherichia coli isolates.…”