Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

Nicolás-Méndez, Tonancy; Kacew, Sam; Ortiz-Muñiz, Rocío; Mendoza-Núñez, Vı́ctor Manuel; Garcia‐Rodriguez, María del Carmen

doi:10.3390/molecules27134028

Cited by 4 publications

(2 citation statements)

References 72 publications

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“…A significant increase in MN frequencies (12-60 h after CrO 3 treatment) demonstrated Cr(VI)-induced genotoxicity. This is consistent with previous findings evaluating Cr(VI)-induced genotoxicity (10,11), and particularly by CrO 3 (12,13). The underlying genotoxic mechanism of the action of Cr(VI) has been linked to the intracellular reduction of Cr(VI) to Cr(III).…”

Section: Discussionsupporting

confidence: 92%

Evaluation of Clastogenic/Aneugenic Damage Using the FISH Micronucleus Assay in Mice Exposed to Chromium (VI)

DEL CARMEN GARCÍA-RODRÍGUEZ,

SANTIAGO-MORENO,

MOLINA-ALVAREZ

et al. 2023

In Vivo

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Background/Aim: Exposure to chromium (VI) [Cr (VI)] has been postulated to be associated with the induction of cancer. In vivo studies utilizing biomarkers of genotoxic damage could aid in elucidating the mechanisms underlying the genotoxic effects of Cr(VI) and their relationship with carcinogenesis. In this study, the origin (clastogenic and/or aneugenic damage) and kinetics of micronuclei (MN) induced by Cr(VI) were investigated. Materials and Methods: Hsd:ICR female mice were divided into groups of five individuals each. MN kinetics were measured in groups treated with 20 or 25 mg/kg CrO 3 intraperitoneally using acridine orange-coated slides in peripheral blood obtained from the caudal vein 0, 12, 24, 36, 48, 60, and 72 h after treatment. Whereas identification of MN with centromeric DNA (MNK+) was measured at the dose of 20 mg/kg of CrO 3 , using fluorescence in situ hybridization (FISH) with a centromere-specific probe in peripheral blood obtained at 0, 12, and 48 h after treatment. Control groups were administered vehicle only. Results: Total MN were quantified and the clastogenic/aneugenic effects of Cr(VI) were evaluated based on the proportion of MNK+ versus micronuclei without centromeric DNA (MNK-). There was a significant increase in MN frequencies beginning at 12 h in the Cr(VI)-treated groups demonstrating its genotoxicity. When calculating the MNK+ as a percentage of the total MN, the increase was significant beginning 12 h after treatment. Conclusion: The fact that the MNK+ and MNK-were observed at both evaluation times corroborates Cr(VI) as a genotoxic agent and demonstrates that both clastogenic and aneugenic damages are involved in the formation of MN.

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Section: Discussionsupporting

confidence: 92%

Evaluation of Clastogenic/Aneugenic Damage Using the FISH Micronucleus Assay in Mice Exposed to Chromium (VI)

DEL CARMEN GARCÍA-RODRÍGUEZ,

SANTIAGO-MORENO,

MOLINA-ALVAREZ

et al. 2023

In Vivo

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“…RES reduces Chromium-induced MN frequency and can repair cells. This is due to the polyphenol content and antioxidant properties of RES (Nicolás-Méndez et al, 2022). It was concluded that the dose of RES-tempeh with a dose of 10 mg/kg BW can increase the number of osteocytes.…”

Section: Discussionmentioning

confidence: 98%

Resveratrol-Tempeh reduce micronucleus frequencies bone marrow cells and stimulate osteocyte proliferation in aluminum chloride-induced mice

et al. 2022

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Aluminum (Al) is widely used for water purification, cooking pots, cosmetic and pharmaceutical preparations, toothpaste tubes, and food processing industries. Although the transport in the digestive tract is very poor but if the load is high, it can be absorbed and accumulated. About 50-70% of Al accumulates in the bones and can have an impact on human health. Resveratrol (RES), isolated from tempeh as an Indonesian food ingredient, can increase cell viability and has promising cytoprotective effects. RES has the capacity to interact with oxidative stress, so it has the potential as a therapy in bone repair. Therefore, this study aimed to evaluate the effect of RES on the number of osteocytes and bone marrow cells in Al-induced mice. Swiss Webster mice were divided into four groups: (1) untreated groups, (2) AlCl3-treated groups, (3) Al+Res5 treated groups, and (4) Al+Res10 treated groups. Al dose 200 mg/kg body weight was administered intraperitoneally. RES was given one hour after administration of Al, with doses of 5 and 10 mg/kg Body Weight. Al and RES administration is carried out for one month. All mice were sacrificed, and mouse bones were isolated for histological preparations and a half for genotoxic assays. Bone marrow cells were collected and stained with My Grunwald. The number of micronuclei polychromatic erythrocytes (MNPCE) was examined in 1,000 PCEs per animal. The number of PCEs is counted by at least 200 erythrocytes (PCE + NCE) per animal. The results showed that the administration of Al significantly increased the number of micronuclei (MN) but after administration of RES at doses of 5 and 10 mg/kg Body Weight significantly reduced the number of MN in bone marrow cells. A dose of RES 10 mg/kg BW stimulates proliferation and increases the number of osteocytes in bone significantly. It can be concluded that Al can cause genotoxicity in bone marrow cells and RES is anti-genotoxic and can stimulate osteocyte proliferation.

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Using Small Molecules for Targeting Heavy Metals in Neurotoxicity and Neuroinflammation

Biswas,

2024

Methods in Molecular Biology

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Protective Effect of Resveratrol against Hexavalent Chromium-Induced Genotoxic Damage in Hsd:ICR Male Mice

Cited by 4 publications

References 72 publications

Evaluation of Clastogenic/Aneugenic Damage Using the FISH Micronucleus Assay in Mice Exposed to Chromium (VI)

Evaluation of Clastogenic/Aneugenic Damage Using the FISH Micronucleus Assay in Mice Exposed to Chromium (VI)

Resveratrol-Tempeh reduce micronucleus frequencies bone marrow cells and stimulate osteocyte proliferation in aluminum chloride-induced mice

Using Small Molecules for Targeting Heavy Metals in Neurotoxicity and Neuroinflammation

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