Protective effect of resveratrol against 6-hydroxydopamine-induced impairment of renal p-aminohippurate transport

Abstract

In the present study, the effects of resveratrol on 6-hydroxydopamine (6-OHDA)-induced p-aminohippurate (PAH) transport impairment were investigated in vitro using rat renal cortical slices. Cisplatin and cephaloridine (CPH), known nephrotoxins, were used as positive controls. In one series of experiments, renal cortical slices were incubated in a cisplatin-containing medium or a cisplatin-free medium. In another series of experiments, renal cortical slices were incubated in a CPH-containing medium, in a CPH- and probenecid-containing medium, or in a CPH-free medium. Subsequently, for each series of experiments kidney slices were incubated in a media containing PAH or tetraethylammonium. In a further series of experiments, renal cortical slices were incubated in a 6-OHDA-containing medium and in a 6-OHDA-free medium. In another series of experiments, renal cortical slices were incubated in a medium containing 50 micro M 6-OHDA, in a 6-OHDA- and resveratrol-containing medium or in a 6-OHDA- and resveratrol-free medium. Subsequently, for each series of experiments kidney slices were incubated in media containing PAH. The results of this study in which slices were incubated in 6-OHDA-containing media indicate that 6-OHDA induced a time- and concentration-dependent decrease in PAH accumulation by renal cortical slices. Resveratrol inhibited the 6-OHDA-induced time-dependent decrease of PAH accumulation in a concentration-dependent manner. Therefore, 6-OHDA causes functional injuries of renal proximal tubule cell membrane, thus leading to impairment of transport processes across the cell membrane and to nephrotoxicity. Resveratrol has a nephroprotective effect.

The sympathetic nervous system regulates skeletal muscle motor innervation and acetylcholine receptor stability

“…To achieve this goal, we performed a state‐of‐the‐art microsurgical procedure to selectively remove sympathetic innervation of mouse hindlimb muscles without interfering with central sympathetic nuclei function, cardiac sympathetic innervation, the sympatho‐adrenomedullary system, or motor axons innervating the skeletal muscle. In contrast to the classical model of muscle denervation, in which sectioning the sciatic nerve or any of its branches engages sympathetic, sensory, and motor axons, and unlike prolonged chemical procedures, such as systemic 6(OH)DA administration, which induce sympathetic denervation, our procedure selectively depletes hindlimbs supplied exclusively by L2‐L3 paravertebral sympathetic ganglia …”

Effects of the Licorice Extract against Tumor Growth and Cisplatin-Induced Toxicity in a Mouse Xenograft Model of Colon Cancer

“…5,6) In addition, recent studies suggest that dietary antioxidants and some foods, such as black grapes and Brassica rapa, can attenuate cisplatin-induced nephrotoxicity and hepatotoxicity. 28,29) The present study was designed to estimate the protective potential of the licorice extract, including speciesspecific antioxidative flavonoids, against cisplatin-induced nephrotoxicity and hepatotoxicity and whether its use influences the therapeutic efficacy of cisplatin, as well as its antitumor activity in an animal model.…”

Protective Effect of the Ethanol Extract of the Roots of Brassica rapa on Cisplatin-Induced Nephrotoxicity in LLC-PK1 Cells and Rats

“…28) In addition, it has been proposed that some foods, such as black grape, which contains resveratrol can provide significant protection against cisplatin-induced nephrotoxicity. 29) Epidemiological studies have shown that brassica vegetables, including cabbages, kale, Brussels sprouts and broccoli can play protective roles against several cancers.…”