Protective effect of rutin on hexachlorobutadiene-induced nephrotoxicity

Abstract

Hexachlorobutadiene (HCBD) is a potent nephrotoxin which nowadays contaminates human foods and water. On the other hands, it has been reported that rutin is a chemopreventive flavonoid which exerts some protective effects on the kidney. Therefore, in this work, the possible effect of rutin on HCBD-induced nephrotoxicity was investigated in female rats. The animals were divided into five groups. Groups 1 and 2 were treated with vehicle and HCBD (100 mg/kg, i.p.), respectively. Groups 3-5 were pretreated with rutin (100, 500 and 1000 mg/kg, i.p.) 1 h before HCBD injection. The level of serum urea and creatinine as well as urinary glucose and protein were measured. Total thiol content and lipid peroxidation level were also determined in the kidney homogenate. When compared to control group, a significant increase in the level of serum creatinine and urea (p < 0.001) as well as urine glucose and protein (p < 0.001) were observed after 24 h of HCBD administration. HCBD also caused a significant decrease in total thiol content (p < 0.001) and a significant increase in lipid peroxidation level (p < 0.001). Pretreatment with rutin could decrease serum creatinine (p < 0.001) and urea (p < 0.001) as well as urine protein (p < 0.001) concentrations when compared with HCBD treated rats. No significant modification on urine glucose was seen (p > 0.05). Rutin also reversed the HCBD-induced depletion in thiol content (p < 0.001) and elevation in lipid peroxidation (p < 0.001) in the kidney. The results of present study showed that rutin clearly attenuated HCBD-induced nephrotoxicity and has the potential to be considered as a nephroprotective agent.

Evaluation of Rheum Turkestanicum in Hexachlorobutadien-Induced Renal Toxicity

“…Also emodin which is found in rheum genus has nephroprotective effects against metals by inhibition of proliferation of renal mesangial cells (MSCs) and thus lower cellular DNA [25]. In addition, rutin as an active ingredient in rheum genus [26] showed nephroprotective effect against cisplatin [27] and hexachlorobutadien [6]. Isolated anthraquinones from rheum genus revealed protective effects against mercuric chloride in rats [28].…”

“…tenuated the nephrotoxicity of HCBD such as safranal [5], rutin [6], pomegranate seed oil [7]. Our recent studies have shown Rheum turkestanicum has antidiabetic effect [8] and cardio protective effect against doxorubicin [9,10].…”

The effect of adipose derived stromal cells on oxidative stress level, lung emphysema and white blood cells of guinea pigs model of chronic obstructive pulmonary disease

“…Total thiol groups were measured using 2,2′-dinitro-5,5′-dithiodibenzoic acid (DTNB) which reacts with the SH groups to produce a yellow colored complex with peak absorbance at 412 nm [23,24]. In summary, 1 mL Tris-EDTA buffer was added to 50 μL of BALF and sample absorbance was read at 412 nm against Tris-EDTA buffer alone (A1).…”

Conjugated metabolites represent the major circulating forms ofAbelmoschus manihot in vivoand show an altered pharmacokinetic profile in renal pathology

“…There are several flavonoids such as rutin (Sadeghnia et al, 2013), naringin (Sahu et al, 2014), and herbs containing flavonoids such as date fruit (El Arem et al, 2014), Murraya paniculata (Zou et al, 2014) exhibit nephroprotective activity, these flavonoids might undergo glucuronide-sulfate conjugate modification in vivo. Our results showed that the relationship between flavonoid glucuronide-sulfate conjugates and nephroprotective activity need further studied.…”