Protective effect of salicylates against hydrogen peroxide stress in yeast

Abstract

Aims: To investigate the effects of salicylates in Saccharomyces cerevisiae exposed to oxidative stress induced by hydrogen peroxide (H2O2). Methods and Results: Saccharomyces cerevisiae was cultured through to the postlogarithmic phase of growth. Stress was induced by the addition of 1·5 mmol l−1 H2O2 for 1 h, while N‐acetyl‐l‐cysteine (NAC) and glutathione (GSSG) were used as control agents that affect the redox balance. Sodium salicylate, at 0·01–10 mmol l−1or acetylsalicylic acid, at 0·02–2·5 mmol l−1 was administered at various times before hydrogen peroxide stress. Both agents conferred resistance to a subsequent hydrogen peroxide stress, similarly to the induction of the adaptive response observed upon pretreatment with NAC and GSSG. Sodium salicylate was more potent as a short‐term, but not as a long‐term pretreatment agent, compared to acetylsalicylic acid. Conclusions: Pharmacological pretreatment with salicylates resulted in dose related increases in cell survival, indicating the induction of the protective response in yeast. Significance and Impact of the study: The possible role of salicylates in the modulation of the hydrogen peroxide stress response in eukaryotic cells address questions on the effects of these commonly used therapeutic agents in a number of disorders exhibiting an oxidative stress component.

Aspirin-induced apoptosis of yeast cells is associated with mitochondrial superoxide radical accumulation and NAD(P)H oxidation

“…This is consonant with the concept that oxidative stress, however produced, regulates the level of apoptosis in yeast (Madeo et al, 1999). On the other hand, there is also evidence in support of aspirin acting as a cytoprotective antioxidant by scavenging of ROS (Yiannakopoulou & Tiligada, 2009;Baltazar et al, 2011). This dichotomous nature of aspirin as both pro-oxidant and antioxidant is reflected in various conflicting reports on effects of aspirin on the superoxide dismutases (SODs) and other antioxidant enzymes.…”

Too much of a good thing: Long-term treatment with salicylate strengthens outer hair cell function but impairs auditory neural activity

“…Salicylate has antioxidant properties and has been used to protect cells from oxidative stress (Althaus et al, 1993; Kojda, 2004; Speir et al, 1998; Yiannakopoulou et al, 2009). Several studies have found that salicylate protects against ototoxicity and noise-induced hearing loss (Chen et al, 2007; Kopke et al, 2000; Li et al, 2002; Sha et al, 1999; Sha et al, 2006; Yamashita et al, 2005).…”

Omega-3 polyunsaturated fatty acids as an angelus custos to rescue patients from NSAID-induced gastroduodenal damage

“…However, the cellular prooxidant behavior of NSAIDs has often been the subject of controversy owing to conflicting reports that NSAIDs such as indomethacin and sulindac scavenge ROS and exert a cytoprotective and antioxidant effect in cells [68,69]. It has also been shown that low doses of aspirin can confer long-term cytoprotective resistance against H 2 O 2 -induced oxidative stress in Saccharomyces cerevisiae cells [70]. Nevertheless, it has been accepted that NSAIDs can induce the proapoptotic accumulation of ROS in mammalian cells.…”