Protective Effect of Selected Medicinal Plants against Hydrogen Peroxide Induced Oxidative Damage on Biological Substrates

Abstract

Oxidative stress is developed due to susceptibility of biological substrates to oxidation by generation of free radicals. In degenerative diseases, oxidative stress level can be reduced by antioxidants which neutralize free radicals. Primary objective of this work was to screen four medicinal plants, namely, Andrographis paniculata, Costus speciosus, Canthium parviflorum, and Abrus precatorius, for their antioxidant property using two biological substrates-RBC and microsomes. The antioxidative ability of three solvent extracts, methanol (100% and 80%) and aqueous leaf extracts, was studied at different concentrations by thiobarbituric acid reactive substances method using Fenton's reagent to induce oxidation in the substrates. The polyphenol and flavonoid content were analyzed to relate with the observed antioxidant effect of the extracts. The phytochemical screening indicated the presence of flavonoids, polyphenols, tannins, and -carotene in the samples. In microsomes, 80% methanol extract of Canthium and Costus and, in RBC, 80% methanol extract of Costus showed highest inhibition of oxidation and correlated well with the polyphenol and flavonoid content. From the results it can be concluded that antioxidants from medicinal plants are capable of inhibiting oxidation in biological systems, suggesting scope for their use as nutraceuticals.

Medicinal plants of the Western ghats as possible inhibitors of oxidation in various biological lipid substrates

“…In cholesterol, the antioxidant activity of CS Aq at higher concentration was 73.75% which is higher than the methanol extracts (Pai Kotebagilu et al, 2015). This protective action of CS Aq may be contributed to the water soluble phytochemicals such as vitamin C, polyphenols, glutathione and tannins (Pai Kotebagilu et al, 2014). Various studies have reported anti-helminthic and larvicidal activity of aqueous extract of CS leaves (Srivastava et al, 2013;Kanakkanath et al, 2013).…”

“…The antioxidant effect of these medicinal plants in biological substrates such as microsomes, red blood cells (Pai Kotebagilu et al, 2014) and lipid substrates such as cholesterol, low density lipoprotein and brain homogenate was evaluated and positive results were obtained (Pai Kotebagilu et al, 2015). This study was conducted in continuation with our previously published work (Pai Kotebagilu et al, 2015) with the major objective of evaluating the antioxidant activity, using aqueous extracts of the AnP, CS, CP and AP.…”

“…In our laboratory, plants such as Morus indica (Arabshahi-Dlouee and Urooj, 2007), Ziziphus jujuba Mill (Esteki and Urooj, 2012) and Moringa oleifera (Reddy et al, 2005) and medicinal plants of the Western ghats such as Andrographis paniculata (AnP) (Pai Kotebagilu et al, 2014;Pai Kotebagilu et al, 2015), Costus speciosus (CS) (Pai Kotebagilu et al, 2014;Pai Kotebagilu et al, 2015), Canthium parviflorum (CP) (Pai Kotebagilu et al, 2014; and Abrus precatorius (AP) (Pai Kotebagilu et al, 2014; have been evaluated for various biological activities by in vitro and in vivo experiments. This study was conducted as part of our attempt to explore the antioxidant activity of medicinal plants of the Western ghats in different food and biological systems.…”

Effect of Bacoside a on Lipid Peroxidation in D-Galactose Induced Aging Mice

“…A large amount of secondary metabolites are present in medicinal plants. Flavonoids, saponins and polyphenols in plants are a versatile group of antioxidants that protect against damage caused due to lipid peroxidation by directly neutralizing reactive oxygen species [15][16][17][18][19][20][21][22]. It is therefore important to study the effect of secondary metabolites on a particular tissue.…”

Short Communication: Augmentation of cardioprotective effect of captopril by Costus speciosus against isoproterenol induced myocardial toxicity in rats

“…It was rated as third plant with potent antioxidant effect among eighteen commonly used antioxidant plants (Lee et al 2015). It is also reported to reduce hydrogen peroxide induced oxidative damage on biological substrates (Pai Kotebagilu et al 2014) and also prevent development of cancer due to its antioxidant action (Baskar et al 2012). The antioxidant effect of this plant is directly related to its phenolic contents (Vijayalakshmi and Sarada 2008;Nehete et al 2010).…”