Protective effect of silymarin on the testes of rats treated with anabolic androgenic steroid: A biochemical, histological, histochemical and immunohistochemichal study

Abstract

Background: Many harmful effects have been reported for the abuse of testosterone with supraphysiologic doses on the male genital system. Objective: To study the possible protective effect of silymarin in testosterone induced testicular changes. Materials and Methods: Eighty adult male albino rats were randomly divided into four groups; untreated rats (controls), rats treated with silymarin, rats treated with testosterone and rats treated with testosterone and silymarin. Relative testicular weight, serum testosterone and FSH were estimated in addition to histological, histochemical and immunohistochemical assessment. Results: Serum testosterone was significantly higher in testosterone treated rats compared with the controls and rats treated with testosterone plus silymarin. Serum FSH was significantly lower in testosterone treated rats as well as rats treated with silymarin plus testosterone compared with controls. There was a significant increase in caspase-3 expression between testosterone treated rats and the other studied groups (P<0.001). There was a significant increase in P53 expression between the testosterone treated rats and the control groups (P<0.001 for each), and also between the testosterone treated rats and rats treated with testosterone and silymarin (P=0.004). P53 expression was also significantly different between rats treated with testosterone plus silymarin and the control groups (P=0.012 for each). Conclusion: Oral concomitant treatment with silymarin was effective in attenuating testosterone-induced testicular damage in adult male albino rats.

Effect of Silymarin as an Adjunct Therapy in Combination with Sofosbuvir and Ribavirin in Hepatitis C Patients: A Miniature Clinical Trial

“…By assuming antioxidant action of silymarin and evaluating the impact of silibinin, which is the most effective flavonoid of silymarin, a significant improvement in testosterone and testicular-related factor was observed [ 63 ]. The results of our investigation are upheld by previous investigations conducted in animal models, which reveal that silymarin is by all means adequately effective to regulate the level of LH, FSH, gonadotropin-releasing hormone, testosterone, and fertility [ 64 – 66 ].…”

The effects of hydroalcoholic extract of Vaccinium arctostaphylos L. on sperm parameters, oxidative injury and apoptotic changes in oxymetholone‐induced testicular toxicity in mouse

“…In monkey, testicular AAS (testosterone undecanoate) up‐regulated the Bax and p‐53, and down‐regulated Bcl‐2 genes over a period of 60 days, and increased apoptosis in spermatogonia cells (Zhang, Zhou, Wei, Hu, & Liu, ). Furthermore, AAS (testosterone) in the rat's testes increased caspase‐3, p‐53 and apoptosis in spermatogonia cells (El‐hanbuli, Abo‐sief, & Mostafa, ).…”

Protective effects of Lepidium draba L . leaves extract on testis histopathology, oxidative stress indicators, serum reproductive hormones and inflammatory signalling in oxymetholone‐treated rat

“…All sections were evaluated at 400 X by utilising Olympus light microscopy (Olympus IX71 microscope) connected to a Moticam (Moticam Technologies) camera system light microscopy system (El‐hanbuli et al., 2017).…”