Protective effect of some plant oils on diazinon induced hepatorenal toxicity in male rats

Abstract

Environmental pollution and exposure to environmental pollutants are still some of the major global health issues. Pesticides have been linked to a wide range of health hazards. The toxicity of pesticides depends on several factors such as its chemical properties, doses, exposure period, exposure methods, gender, genetics, age, nutritional status and physiological case of exposed individuals. Medicinal plants, natural products and nutrition continue to play a central role in the healthcare system of large proportions of the world's population. Alternative medicine plays an important role in health services around the world. The aim of this study was to investigate the effect of olive, sesame and black seed oils on hepatorenal toxicity induced by diazinon (DZN) in male rats. The experimental animals were divided into nine groups. The first group served as control. The second group was exposed to DZN. The third group was treated with olive oil and DZN. Rats of the fourth group were subjected to sesame oil and DZN. Rats of the fifth group were exposed to black seed oil and DZN. The sixth, seventh and eighth groups were supplemented with olive, sesame and black seed oils respectively. Rats of the ninth group were treated with corn oil. Levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, total bilirubin, creatinine, blood urea nitrogen and malondialdehyde were significantly increased in rats exposed to DZN. Moreover, levels of serum glutathione and superoxide dismutase were significantly decreased. Several histopathological changes were observed in the structures of liver and kidney due to DZN exposure. This study showed that these oils attenuated the physiological disturbances and histopathological alterations induced by DZN intoxication. Moreover, the antioxidant properties of these oils support the bioactive roles of its protective effects on DZN toxicity. This study therefore suggests that these oils could be used as preventive factors against the toxicity of DZN due to its antioxidant properties.

The Potential Protective Effect of Sesame Oil and Canola Oil on Rats Exposed to Malathion

“…Furthermore, the antioxidant activities of sesame oil and canola oil were confirmed by the present evaluations of oxidative stress markers in rats subjected to malathion toxicity. The protective effects of sesame oil against several chemical toxicants associated with inhibition of the oxidative stress in experimental animals were reported by previous investigations (Liu et al, 2015;, Al-Attar et al, 2017. Al-Attar et al (2017; showed that the sesame oil attenuated the physiological disturbances and histopathological alterations induced by diazinon intoxication in male rats.…”

Effect of Soyflour (Glycine max L.) Hepatoprotector Activity on Liver MDA Level in Male Wistar Rat Induced by Diazinon

“…Beberapa penelitian menunjukkan diazinon bersifat hepatotoksik pada tikus. Diazinon meningkatkan kadar serum alkalin fosfatase, alanin transaminase, aspartat trasaminase, gamma glutamyl transferase, bilirubin total, kreatinin, dan malondialdehid (Al-Attar et al, 2016). Diazinon mengandung radikal bebas yang menyebabkan stres oksidatif dan menginduksi terjadinya peroksidasi lipid (Oksay, 2012).…”

“…Hepatoprotektor bekerja dengan mencegah adanya ikatan hidroksil yang diakibatkan oleh radikal bebas. Hepatoprotektor dapat ditemukan secara alami pada tumbuhan yang mengandung senyawa antioksidan (Al-Attar et al, 2016). Senyawa antioksidan ini diyakini dapat menetralkan efek negatif radikal bebas pada sel-sel tubuh.…”

“…Hasil ini sesuai dengan penelitian sebelumnya bahwa pemberian diazinon dengan dosis 15 mg/kgBB secara oral selama empat minggu pada tikus dapat menyebabkan peningkatan MDA hati tikus (Lari et al, 2013). Penelitian serupa lainnya menunjukkan bahwa diazinon dapat meningkatkan kadar serum alkalin fosfatase, alanin transaminase, aspartat trasaminase, gamma glutamyl transferase, bilirubin total, kreatinin, dan MDA, sehingga menunjukkan adanya perubahan histopatologi dan biokimia hati (Al-Attar et al, 2016). Diazinon diubah menjadi bentuk intermediet aktif diazoxon oleh sitokrom hati P450s melalui pelepasan sulphur yang menempel pada fosfor dan insersi atom oksigen (desulfurasi oksidatif) (Abass et al, 2012).…”

Сlinical signs and pathoanatomical changes in the brain for poisoning of domestic turkeys with diazinon

“…За аліментарного потрапляння діазинону в організм відбувається швидке його всмоктування у шлунково-кишковому тракті. У організмі діазинон метаболізує з утворенням токсичного метаболіту діазоксону (Aleksandrova and Goncharenko, 1983;Cox, 2000;Majeed and Al-Sereah Bahaa, 2012;Alam et al, 2015;Dankovych and Tumanov, 2016;Al-Attar et al, 2017).…”