Protective Effect of Sundakai (Solanum torvum) Seed Protein (SP) Against Oxidative Membrane Damage in Human Erythrocytes

Abstract

Lipid peroxidation by ROS at the membrane level disturbs the inherit integrity of components activating subsequent alterations in the function. In this study, the protective effect of purified Sundakai (Solanum torvum) seed protein (SP) was tested against oxidative membrane damage in erythrocyte membrane. SP prevented oxidative RBC lysis induced by pro-oxidants; Fe:As (2:20 μmol), periodate (0.4 mM), and t-BOOH (1 mM) up to 86, 81, and 86 %, respectively. Further, SP prevented the Fe:As-induced K(+) leakage up to the tune of 95 %. The inhibition offered by SP on K(+) leakage was comparable to inhibition offered by quinine sulfate, a known K(+) channel blocker. SP dose dependently restored Na(+)K(+) ATPase and Ca(2+)Mg(2+) ATPase activities in erythrocyte membrane. The restoration of ATPase activity by SP was two times more than standard antioxidants BHA and α-tocopherol. Besides, SP at 1.6 μmol restored the membrane proteins over Fe:As induction when analyzed by SDS-PAGE, which was comparable to protection offered by BHA. In conclusion, SP is an effective antioxidant in preventing oxidative membrane damage and associated functions mediated by ROS. As SP is non-toxic, it can be used as an effective bioprotective antioxidant agent to cellular components.

Comparative Investigation of the Biological Activity of the Pterocarpus osun and Bosqueia angolensis Seeds Protein Extracts

“…Currently, the hunt for natural products with antioxidant potentials have been on the rise due to the risk of adverse effects encountered with the use of synthetic compounds and also the public's perception that natural and dietary antioxidant are safer than synthetic analogues (Manukumar and Madhu, 2013). The growing of medical interest also in the search for novel natural and dietary antioxidant had lead to the increasing reports of the existence and bioactivities of antioxidant proteins in plants (Prasad et al, 2010;Sivapriya et al, 2015;Ramadas et al, 2016). Proteins having significant antioxidant activity have been isolated from plants sources such as Solanum torvum seeds (Sivapriya et al, 2015), wheat germ (Mahmoud et al, 2015), Cajanus indicus leaves (Sarkar et al, 2006), Cajanus cajan seed coat (Manukumar and Madhu, 2013), Lablab purpureous seeds (Saha et al, 2014), Curcuma species (Boonmee et al, 2011;Angel et al, 2013), Peganum harmala seeds (Ahmed et al, 2013), Terminalia chebula fruit (Srivastava et al, 2012), Moringa oleifera seeds (Santos et al, 2005) and Ginkgo biloba seeds (Zhou et al, 2012).…”