Protective effect of Thunbergia laurifolia extract on hemolysis during Plasmodium berghei infection

Khobjai, Warachate; Jaihan, Ubonwan; Watcharasamphankul, Worawan; Somsak, Voravuth

doi:10.1007/s00436-014-3831-y

Cited by 9 publications

(6 citation statements)

References 12 publications

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“…[ 26 ] The extract also demonstrated anti-inflammatory. [ 13 16 18 19 ] There was no edema or congestion on day 3, postoperation in all TLL gel treated groups but these signs of inflammation were observed in the untreated burn group. Congestion was observed until day 7 in the untreated group.…”

Section: Discussionmentioning

confidence: 93%

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Evaluation of wound healing activity of Thunbergia laurifolia supercritical carbon dioxide extract in rats with second-degree burn wounds

Kwansang

Itthipanichpong

Limpanasithikul

2015

J Adv Pharm Technol Res

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Thunbergia laurifolia Lindl (TL) has been traditionally used as an antidote, anti-inflammatory, and anti-drug addiction. This study investigated the burn wound healing activity of TL leaf extract (TLL) from supercritical CO2 extraction in rats. The extract was prepared to 2.5%, 5%, and 10% gel (TLL gel). Rats were induced to second-degree burn wounds. They were randomly divided into six groups (six rats/group), which five groups were topically applied gel base, 1% silver sulfadiazine gel, 2.5%, 5%, and 10% TLL gel, respectively, for 14 days. Six untreated burn rats were used as the control group. The rats in each group were evaluated for wound healing rate, histological parameters, and wound collagen content. Rats treated with 10% TLL gel had a higher wound healing rate than rats in the control and untreated groups. An increase in collagen content, which indicates good regeneration of wound skin, was observed in the TLL treated rats from a pathological study by Masson's trichrome and collagen content assay. The results from this study suggest that T. laurifolia leaf extract obtained by supercritical CO2 extraction promotes the recovery of wound skin by shortening the inflammation phase, increasing collagen content, and stimulating fibroblasts proliferation and migration in wound healing.

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Section: Discussionmentioning

confidence: 93%

“…[ 12 ] Reported activities of T. laurifolia leaves include antioxidant, anti-inflammatory, anti-drug addiction, antidote, hepatoprotective, and anti-diabetic activities. [ 13 14 15 16 17 18 19 ] We intended to explore the wound healing effect of these leaves.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of wound healing activity of Thunbergia laurifolia supercritical carbon dioxide extract in rats with second-degree burn wounds

Kwansang

Itthipanichpong

Limpanasithikul

2015

J Adv Pharm Technol Res

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“…Additionally, reduction of PCV during malaria infection is also considered. The PCV of untreated control was reduced because of PbANKA infection leading to rapid hemolysis (Khobjai et al 2014;Zhu et al 2015). The absence of signi cant PCV reduction among DHA/GIE combination-treated mice at the doses of ratios 60/40, 40/60, 20/80, and 0/100 may indicate the protective effect of GIE on PCV reduction during malaria infection.…”

Section: Discussionmentioning

confidence: 95%

Antimalarial potential of Gymnema inodorum leaf extract and dihydroartemisinin combination against Plasmodium berghei infected mice

Somsak

Ounjaijean

2022

Preprint

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Chemotherapy plays a crucial role in malaria control. However, the main obstacle to treatment has been the rise of parasite resistance to most antimalarial drugs. Artemisinin-based combination therapies (ACTs) remain today’s most effective antimalarial therapies. However, malaria parasite tolerance to ACTs is now increasingly prevalent, especially in Southeast Asia. Consequently, this creates the need for effective alternative antimalarials. Interestingly, the effectiveness of Gymnema inodorum leaf extract (GIE) as an antimalarial candidate was reported earlier. Therefore, this study sought to evaluate the antimalarial potential of dihydroartemisinin (DHA) based combination therapy with GIE against Plasmodium berghei in a mouse model. Drug assessment was carried out on P. berghei ANKA (PbANKA) using the standard 4-day test for determination of fifty percent effective dose (ED50) of DHA and GIE individually. Moreover, the combination of DHA and GIE was also evaluated based on fixed-ratio strategy, 100/0, 80/20, 60/40, 40/60, 20/80, and 0/100 of ED50 of DHA and GIE, respectively. Overall, 2 mg/kg and 100 mg/kg of DHA and GIE resulted in ED50 against PbANKA. In combination, the ratio of 60/40 (DHA/GIE) had a considerable the highest antimalarial activity significantly (p < 0.001) against PbANKA with 88.95% inhibition that indicated a synergism efficacy (CI value = 0.68695). Additionally, this ratio was protected PbANKA infected mice from BW loss and PCV reduction with prolonged survival time throughout the 30 day follow up. This study points to better efficacy of GIE as an alternative drug partner in combination to enhance antimalarial efficacy of DHA against PbANKA infected mice.

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“…Thunbergia laurifolia Lindl . leaf extract was reported to demonstrate anti‐inflammatory, antioxidant, antimicrobial, hepatoprotective, and antidiabetic activities . Acacia catechu heartwood extract was reported to have antimicrobial, anti‐inflammatory, antidiabetic, and anticancer properties .…”

Section: Discussionmentioning

confidence: 99%

Novel bioactive peptides demonstrating anti‐dengue virus activity isolated from the Asian medicinal plant Acacia Catechu

et al. 2018

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The therapeutic activities of food-derived bioactive proteins and peptides are attracting increased attention within the research community. Medicinal plants used in traditional medicines are an excellent source of bioactive proteins and peptides, especially those traditionally prepared by water extraction for use as tea or food supplement. In this study, novel bioactive peptides were isolated from enzymatic digests of 33 Thai medicinal plants. The inhibitory activity of each against dengue virus (DENV) infection was investigated. Of 33 plants, peptides from Acacia catechu extract demonstrated the most pronounced anti-DENV activity. Half maximal inhibitory concentration of 0.18 μg/ml effectively inhibited DENV foci formation. Treatment with 1.25 μg/ml crude peptide extract could reduce virus production less than 100-fold with no observable cell toxicity. Peptide sequences were determined by high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry. Two bioactive peptides isolated from Acacia catechu inhibited DENV foci formation >90% at the concentration of 50 μM; therefore, they are recommended for further investigation as antiviral peptides against DENV infection.

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Protective effect of Thunbergia laurifolia extract on hemolysis during Plasmodium berghei infection

Cited by 9 publications

References 12 publications

Evaluation of wound healing activity of Thunbergia laurifolia supercritical carbon dioxide extract in rats with second-degree burn wounds

Evaluation of wound healing activity of Thunbergia laurifolia supercritical carbon dioxide extract in rats with second-degree burn wounds

Antimalarial potential of Gymnema inodorum leaf extract and dihydroartemisinin combination against Plasmodium berghei infected mice

Novel bioactive peptides demonstrating anti‐dengue virus activity isolated from the Asian medicinal plant Acacia Catechu

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