Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells)

Calò, Rossella; Marabini, Laura

doi:10.1016/j.jphotobiol.2014.01.013

Cited by 69 publications

(44 citation statements)

References 45 publications

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“…In a HaCat cell line, a water-soluble extract of bilberry prevented UVAand UVB-induced damage [Calò and Marabini, 2014], similarly to melatonin, creatine, and hydroxytyrosol [Salucci et al, 2014]. Another recent study reported that anthocyanin-rich fractions of blackberry extracts reduced UV-induced ROS in human keratinocyte primary cultures in a dose-dependent manner only if added immediately before or after irradiation, but not 24 h after irradiation [Murapa et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

Effects of UV Rays and Thymol/Thymus vulgaris L. Extract in an ex vivo Human Skin Model: Morphological and Genotoxicological Assessment

Cornaghi

Arnaboldi

Calò

et al. 2016

Cells Tissues Organs

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Ultraviolet (UV) radiation is the major environmental factor affecting functions of the skin. Compounds rich in polyphenols, such as Thymus vulgaris leaf extract and thymol, have been proposed for the prevention of UV-induced skin damage. We compared the acute effects induced by UVA and UVB rays on epidermal morphology and proliferation, cytotoxicity, and genotoxicity. Normal human skin explants were obtained from young healthy women (n = 7) after informed consent and cultured at the air-liquid interface overnight. After 24 h, the samples were divided in 2 groups: the former exposed to UVA (16 or 24 J/cm²) and the latter irradiated with UVB (0.24 or 0.72 J/cm²). One hour after the end of irradiation, supernatants were collected for evaluation of the lactate dehydrogenase activity. Twenty-four hours after UVB exposure, biopsies were processed for light and transmission electron microscopy analysis, proliferation, cytotoxicity, and genotoxicity. UVB and UVA rays induced early inhibition of cell proliferation and DNA damage compared to controls. In particular, UVB rays were always more cytotoxic and genotoxic than UVA ones. For this reason, we evaluated the effect of either T. vulgaris L. extract (1.82 µg/ml) or thymol (1 µg/ml) on all samples treated for 1 h before UVB irradiation. While Thymus had a protective action for all of the endpoints evaluated, the action of the extract was less pronounced on epidermal proliferation and morphological features. The results presented in this study could be the basis for investigating the mechanism of thymol and T. vulgaris L. extract against the damage induced by UV radiation.

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Section: Discussionmentioning

confidence: 99%

Effects of UV Rays and Thymol/Thymus vulgaris L. Extract in an ex vivo Human Skin Model: Morphological and Genotoxicological Assessment

Cornaghi

Arnaboldi

Calò

et al. 2016

Cells Tissues Organs

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“…The cytokinesis-block MN assay enables better precision as the data acquired are not confounded with altered cell division kinetics caused by cytotoxicity of the agents tested or by sub-optimal cell culture conditions. This methodology has been used to assess the UV-induced DNA damage in keratinocytes [17]. The similarity between both counts shows that the extract itself is not genotoxic.…”

Section: Protective Effect Against Dna Damage Induced By Uv Radiationmentioning

confidence: 99%

“…Similar results were obtained following UV radiation, which suggests that ECS is not phototoxic at the UV doses used (Fig.3). CBMN assay has emerged as one of the favorite methods for evaluating DNA damage since it allows both chromosome loss and chromosome breakage to be measured reliably and has been used to quantify DNA damage in skin cells [17]. Noteworthy, since the European Union regulation nº 1223/2009 has come into force, animal testing of cosmetic and ingredients was banned and the study of their efficacy and safety has move towards in vitro methodologies.…”

Section: Protective Effect Against Dna Damage Induced By Uv Radiationmentioning

confidence: 99%

Protective effect of C. sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line

Almeida

Pinto

Monteiro

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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“…Bilberry, for example, has historically been used as a medicinal herb to treat diarrhea and improve night vision. Analysis of bilberry fruits' composition shows that they are a rich source of nutraceutics like phenolic substances, carotenoids and vitamins [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

Pruchnik

Bonarska-Kujawa

Żyłka

et al. 2018

J Therm Anal Calorim

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The aim of the study was to determine changes incurred in lipid model membranes by polyphenol compounds from blueberry fruit of the genus Vaccinium that includes low, high and black blueberry. In particular, the effect of extracts on the packing order in the lipid hydrophilic phase, the fluidity of the hydrophobic phase, as well as the temperature of the phase transition in phospholipids was studied. Model membranes were formed of dipalmitoylphosphatidylcholine (DPPC) and egg phosphatidylcholine. The interaction of extracts with lipids was studied using the differential scanning calorimetry, infrared spectroscopy (ATR-FTIR) and fluorimetry using Laurdan, Prodan and DPH probes. All experimental results indicate that the biggest changes are in the hydrophilic part of the lipid bilayer. The polyphenol compounds had practically no influence on fluidity in the hydrophobic region of the membranes. No changes in the temperature of the main phase transition of DPPC were observed and only a small change in the temperature of pretransition for high concentration of compounds. Results obtained with the ATR-FTIR method did not reveal any changes in the alkyl chain region of bilayer; however, a small shift of bands was observed for phosphate and choline groups-the broadest effect appeared for the wild bilberry. The findings indicate that polyphenolic compounds contained in extracts of blueberries interact with the membrane surface and therefore can protect it.

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Protective effect of Vaccinium myrtillus extract against UVA- and UVB-induced damage in a human keratinocyte cell line (HaCaT cells)

Cited by 69 publications

References 45 publications

Effects of UV Rays and Thymol/<b><i>Thymus vulgaris</i></b> L. Extract in an ex vivo Human Skin Model: Morphological and Genotoxicological Assessment

Effects of UV Rays and Thymol/<b><i>Thymus vulgaris</i></b> L. Extract in an ex vivo Human Skin Model: Morphological and Genotoxicological Assessment

Protective effect of C. sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line

Application of the DSC and spectroscopy methods in the analysis of the protective effect of extracts from the blueberry fruit of the genus Vaccinium in relation to the lipid membrane

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